microrna microarray analysis (Macrogen)
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Macrogen
microrna microarray analysis
Microrna Microarray Analysis, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microrna+microarray+analysis/microrna+microarray+analysis/pm34263295-18-9-13
Average 90 stars, based on 1 article reviews
Microrna Microarray Analysis, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microrna+microarray+analysis/microrna+microarray+analysis/pm34263295-18-9-13
Average 90 stars, based on 1 article reviews
microrna microarray analysis - by Bioz Stars,
2026-09
90/100 stars
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Isolation:Article Title: RAS-stimulated release of exosomal miR-494-3p promotes the osteolytic bone metastasis of breast cancer cells Article Snippet: The cells were seeded in a cell culture dish (2.5×10 5 cells/ml), cultured for 24 h, and then transfected with nucleic acids (plasmids, 1 μ g per transfection; siRNAs or miRNAs, 10 or 100 μ M) using Lipofectamine 3000 (for plasmids, cat. no. L3000-015) or RNAiMAX (for siRNAs and miRNAs, cat. no. 13778-150) according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.) After 48 h, the transfected cells were used for further experiments. .. Exosomes isolated from the MCF-7 cells transfected with control or KRASV12 were used for Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization Article Snippet: Samples were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA). .. Transfection:Article Title: RAS-stimulated release of exosomal miR-494-3p promotes the osteolytic bone metastasis of breast cancer cells Article Snippet: The cells were seeded in a cell culture dish (2.5×10 5 cells/ml), cultured for 24 h, and then transfected with nucleic acids (plasmids, 1 μ g per transfection; siRNAs or miRNAs, 10 or 100 μ M) using Lipofectamine 3000 (for plasmids, cat. no. L3000-015) or RNAiMAX (for siRNAs and miRNAs, cat. no. 13778-150) according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.) After 48 h, the transfected cells were used for further experiments. .. Exosomes isolated from the MCF-7 cells transfected with control or KRASV12 were used for Control:Article Title: RAS-stimulated release of exosomal miR-494-3p promotes the osteolytic bone metastasis of breast cancer cells Article Snippet: The cells were seeded in a cell culture dish (2.5×10 5 cells/ml), cultured for 24 h, and then transfected with nucleic acids (plasmids, 1 μ g per transfection; siRNAs or miRNAs, 10 or 100 μ M) using Lipofectamine 3000 (for plasmids, cat. no. L3000-015) or RNAiMAX (for siRNAs and miRNAs, cat. no. 13778-150) according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.) After 48 h, the transfected cells were used for further experiments. .. Exosomes isolated from the MCF-7 cells transfected with control or KRASV12 were used for Article Title: Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells Article Snippet: .. sEVs were purified from control or syntenin knockdown NCI-H226 cells and Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization Article Snippet: Samples were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA). .. Article Title: Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells. Article Snippet: .. The cells were then stained with hematoxylin and eosin (H&E), and then counted in at least five randomly selected microscopic fields (×100) per filter. Microarray:Article Title: RAS-stimulated release of exosomal miR-494-3p promotes the osteolytic bone metastasis of breast cancer cells Article Snippet: The cells were seeded in a cell culture dish (2.5×10 5 cells/ml), cultured for 24 h, and then transfected with nucleic acids (plasmids, 1 μ g per transfection; siRNAs or miRNAs, 10 or 100 μ M) using Lipofectamine 3000 (for plasmids, cat. no. L3000-015) or RNAiMAX (for siRNAs and miRNAs, cat. no. 13778-150) according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.) After 48 h, the transfected cells were used for further experiments. .. Exosomes isolated from the MCF-7 cells transfected with control or KRASV12 were used for Article Title: MicroRNAs as potential indicators of the development and progression of uterine leiomyoma. Article Snippet: RNA samples (130 ng) were labeled with the FlashTagTM Biotin RNA Labeling Kit (Genisphere, Hatfield, PA, USA). .. The labeled RNA was quantified, fractionated and hybridized to the Article Title: Comparative analysis of microRNA expression profiles in the colons of specific pathogen-free mice and germ-free mice. Article Snippet: MicroRNAs play an important role in microbiota–host crosstalk.. In this study, we compared microRNA expression in whole colons of specific pathogen-free mice and germ-free mice.. Forty-eight microRNAs were differentially expressed by more than 2-fold. Article Title: Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker Article Snippet: The concentration of extracted RNA was quantified by the NanoDrop 2000 (Thermo Fisher Scientific) and stored at −80 °C before use. .. For the exploration of biomarkers, extracted RNA was analyzed by nucleic acid analysis using microarray and qRT-PCR assays. Article Title: Epstein-Barr virus-encoded miR-BART5-5p upregulates PD-L1 through PIAS3/pSTAT3 modulation, worsening clinical outcomes of PD-L1-positive gastric carcinomas. Article Snippet: Background Epstein–Barr virus (EBV) is etiologically associated with ~ 10% of all gastric carcinomas.. However, the molecular mechanisms and roles of EBV miRNAs in gastric carcinoma oncogenesis are yet to be elucidated.. Methods MicroRNA microarray and TaqMan quantitative real-time RT-PCR were conducted. Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization Article Snippet: Samples were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA). .. Labeling:Article Title: MicroRNAs as potential indicators of the development and progression of uterine leiomyoma. Article Snippet: RNA samples (130 ng) were labeled with the FlashTagTM Biotin RNA Labeling Kit (Genisphere, Hatfield, PA, USA). .. The labeled RNA was quantified, fractionated and hybridized to the Purification:Article Title: Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells Article Snippet: .. sEVs were purified from control or syntenin knockdown NCI-H226 cells and Article Title: Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells. Article Snippet: .. The cells were then stained with hematoxylin and eosin (H&E), and then counted in at least five randomly selected microscopic fields (×100) per filter. Knockdown:Article Title: Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells Article Snippet: .. sEVs were purified from control or syntenin knockdown NCI-H226 cells and Article Title: Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells. Article Snippet: .. The cells were then stained with hematoxylin and eosin (H&E), and then counted in at least five randomly selected microscopic fields (×100) per filter. Quantitative RT-PCR:Article Title: Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker Article Snippet: The concentration of extracted RNA was quantified by the NanoDrop 2000 (Thermo Fisher Scientific) and stored at −80 °C before use. .. For the exploration of biomarkers, extracted RNA was analyzed by nucleic acid analysis using microarray and qRT-PCR assays. Immunohistochemistry:Article Title: Epstein-Barr virus-encoded miR-BART5-5p upregulates PD-L1 through PIAS3/pSTAT3 modulation, worsening clinical outcomes of PD-L1-positive gastric carcinomas. Article Snippet: Background Epstein–Barr virus (EBV) is etiologically associated with ~ 10% of all gastric carcinomas.. However, the molecular mechanisms and roles of EBV miRNAs in gastric carcinoma oncogenesis are yet to be elucidated.. Methods MicroRNA microarray and TaqMan quantitative real-time RT-PCR were conducted. In Situ Hybridization:Article Title: Epstein-Barr virus-encoded miR-BART5-5p upregulates PD-L1 through PIAS3/pSTAT3 modulation, worsening clinical outcomes of PD-L1-positive gastric carcinomas. Article Snippet: Background Epstein–Barr virus (EBV) is etiologically associated with ~ 10% of all gastric carcinomas.. However, the molecular mechanisms and roles of EBV miRNAs in gastric carcinoma oncogenesis are yet to be elucidated.. Methods MicroRNA microarray and TaqMan quantitative real-time RT-PCR were conducted. Plasmid Preparation:Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization Article Snippet: Samples were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA). .. Staining:Article Title: Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells. Article Snippet: .. The cells were then stained with hematoxylin and eosin (H&E), and then counted in at least five randomly selected microscopic fields (×100) per filter. |
