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microarray hybridization chamber  (Agilent technologies)


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    Agilent technologies microarray hybridization chamber
    Microarray Hybridization Chamber, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chambers/pm36768818-416-7-6
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 ◦C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Article Title: Differential gene expression and biomarkers in zebrafish (Danio rerio) following exposure to produced water components.
    Article Snippet: The main effluent from oil and gas production is produced water (PW), a waste that contains low to moderate concentrations of oil-derived substances such as polycyclic aromatic hydrocarbons (PAHs) and alkylphenols (APs).. PW components may be present in seawater at low concentrations over large areas in the vicinity of oil and gas production facilities.. In this study, zebrafish (Danio rerio) were exposed to control and three treatments (high-, pulsed-, low-dose) of a synthetic PW mixture for 1, 7 and 13 weeks.

    Article Title: Differential gene expression in cisplatin-resistant and -sensitive testicular germ cell tumor cell lines
    Article Snippet: .. The labelled samples were hybridized onto the SurePrint G3 Human Gene Expression microarray in the Agilent Microarray Hybridization Chamber (G2534) for 20 h at 65°C/20 rpm in a rotator oven. .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188–5327) and drying, slides were scanned in the Innoscan 900 microarray scanner (Innopsys Inc., Carbonne, France) with the resolution of 3 micrometers.

    Hybridization:

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 ◦C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Article Title: Differential gene expression and biomarkers in zebrafish (Danio rerio) following exposure to produced water components.
    Article Snippet: The main effluent from oil and gas production is produced water (PW), a waste that contains low to moderate concentrations of oil-derived substances such as polycyclic aromatic hydrocarbons (PAHs) and alkylphenols (APs).. PW components may be present in seawater at low concentrations over large areas in the vicinity of oil and gas production facilities.. In this study, zebrafish (Danio rerio) were exposed to control and three treatments (high-, pulsed-, low-dose) of a synthetic PW mixture for 1, 7 and 13 weeks.

    Article Title: Differential gene expression in cisplatin-resistant and -sensitive testicular germ cell tumor cell lines
    Article Snippet: .. The labelled samples were hybridized onto the SurePrint G3 Human Gene Expression microarray in the Agilent Microarray Hybridization Chamber (G2534) for 20 h at 65°C/20 rpm in a rotator oven. .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188–5327) and drying, slides were scanned in the Innoscan 900 microarray scanner (Innopsys Inc., Carbonne, France) with the resolution of 3 micrometers.

    Incubation:

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 ◦C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Gene Expression:

    Article Title: Differential gene expression in cisplatin-resistant and -sensitive testicular germ cell tumor cell lines
    Article Snippet: .. The labelled samples were hybridized onto the SurePrint G3 Human Gene Expression microarray in the Agilent Microarray Hybridization Chamber (G2534) for 20 h at 65°C/20 rpm in a rotator oven. .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188–5327) and drying, slides were scanned in the Innoscan 900 microarray scanner (Innopsys Inc., Carbonne, France) with the resolution of 3 micrometers.



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    microarray hybridization chambers  (Agilent technologies)
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    A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide <t>microarray</t> data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.
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    Image Search Results


    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Journal: BMC Cancer

    Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

    doi: 10.1186/1471-2407-13-364

    Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing

    A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

    Journal: PLoS ONE

    Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression

    doi: 10.1371/journal.pone.0110934

    Figure Lengend Snippet: A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

    Article Snippet: Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008).

    Techniques: Expressing, Microarray, Derivative Assay