microarray hybridization chambers (Agilent technologies)
Structured Review

Microarray Hybridization Chambers, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridization+chambers/pmc04217762-226-10-9
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression"
Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression
Journal: PLoS ONE
doi: 10.1371/journal.pone.0110934
Figure Legend Snippet: A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.
Techniques Used: Expressing, Microarray, Derivative Assay
Related Articles
Microarray:Article Title: Multi-Omics Analysis of Western-style Diet Increased Susceptibility to Experimental Colitis in Mice Article Snippet: The labeled RNAs were amplified and transcribed into cRNA using RNeasy Mini Kit (74104, Qiagen). .. The fluorescent cRNAs were hybridized onto the Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression Article Snippet: .. Arrays were incubated at 65°C for 17 h in Article Title: Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets Article Snippet: Microarray hybridizations were carried out on labelled cRNAs with specificity greater than 8 using the in situ Hybridization Kit Plus (Agilent Technologies). .. Arrays were incubated at 65°C for 17 h in Article Title: Transcript Profiling Identifies Dynamic Gene Expression Patterns and an Important Role for Nrf2/Keap1 Pathway in the Developing Mouse Esophagus Article Snippet: Samples were moved to ice, and fragmentation was stopped by addition of 125 μl of Agilent's 2× in situ hybridization buffer. .. Microarrays were hybridized in Article Title: Network-Based Gene Expression Biomarkers for Cold and Heat Patterns of Rheumatoid Arthritis in Traditional Chinese Medicine Article Snippet: .. The arrays were incubated at 65°C for 17 h in Article Title: Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival Article Snippet: .. This “hybridization sandwich” was assembled in an Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes. Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Article Title: Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats Article Snippet: All these steps were done using Gene expression hybridization kit (Agilent Technologies, Palo Alto, CA). .. 48 μl of these mixes were used for the hybridization, which was done in Hybridization:Article Title: Multi-Omics Analysis of Western-style Diet Increased Susceptibility to Experimental Colitis in Mice Article Snippet: The labeled RNAs were amplified and transcribed into cRNA using RNeasy Mini Kit (74104, Qiagen). .. The fluorescent cRNAs were hybridized onto the Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression Article Snippet: .. Arrays were incubated at 65°C for 17 h in Article Title: Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets Article Snippet: Microarray hybridizations were carried out on labelled cRNAs with specificity greater than 8 using the in situ Hybridization Kit Plus (Agilent Technologies). .. Arrays were incubated at 65°C for 17 h in Article Title: Transcript Profiling Identifies Dynamic Gene Expression Patterns and an Important Role for Nrf2/Keap1 Pathway in the Developing Mouse Esophagus Article Snippet: Samples were moved to ice, and fragmentation was stopped by addition of 125 μl of Agilent's 2× in situ hybridization buffer. .. Microarrays were hybridized in Article Title: Network-Based Gene Expression Biomarkers for Cold and Heat Patterns of Rheumatoid Arthritis in Traditional Chinese Medicine Article Snippet: .. The arrays were incubated at 65°C for 17 h in Article Title: Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival Article Snippet: .. This “hybridization sandwich” was assembled in an Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes. Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Article Title: Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats Article Snippet: All these steps were done using Gene expression hybridization kit (Agilent Technologies, Palo Alto, CA). .. 48 μl of these mixes were used for the hybridization, which was done in Incubation:Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression Article Snippet: .. Arrays were incubated at 65°C for 17 h in Article Title: Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets Article Snippet: Microarray hybridizations were carried out on labelled cRNAs with specificity greater than 8 using the in situ Hybridization Kit Plus (Agilent Technologies). .. Arrays were incubated at 65°C for 17 h in Article Title: Network-Based Gene Expression Biomarkers for Cold and Heat Patterns of Rheumatoid Arthritis in Traditional Chinese Medicine Article Snippet: .. The arrays were incubated at 65°C for 17 h in Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes. Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Gene Expression:Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression Article Snippet: .. Arrays were incubated at 65°C for 17 h in Formalin-fixed Paraffin-Embedded:Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes. Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Binding Assay:Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes. Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in |
