Review



microarray hybridization chambers  (Agilent technologies)


Bioz Verified Symbol Agilent technologies is a verified supplier
Bioz Manufacturer Symbol Agilent technologies manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Agilent technologies microarray hybridization chambers
    A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide <t>microarray</t> data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.
    Microarray Hybridization Chambers, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chambers/pmc04217762-226-10-9
    Average 90 stars, based on 1 article reviews
    microarray hybridization chambers - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression"

    Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0110934

    A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.
    Figure Legend Snippet: A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

    Techniques Used: Expressing, Microarray, Derivative Assay

    Related Articles

    Microarray:

    Article Title: Multi-Omics Analysis of Western-style Diet Increased Susceptibility to Experimental Colitis in Mice
    Article Snippet: The labeled RNAs were amplified and transcribed into cRNA using RNeasy Mini Kit (74104, Qiagen). .. The fluorescent cRNAs were hybridized onto the microarray hybridization chambers (G2530-60029, Agilent Technologies). .. After the microarray was washed, an Agilent DNA Microarray Scanner (G2565BA, Agilent Technologies) was used to scan the signals.

    Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression
    Article Snippet: .. Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008). .. Gene expression profiles were evaluated using Agilent whole rat genome 4×44K oligonucleotide microarrays.

    Article Title: Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets
    Article Snippet: Microarray hybridizations were carried out on labelled cRNAs with specificity greater than 8 using the in situ Hybridization Kit Plus (Agilent Technologies). .. Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to the Agilent protocol. .. Arrays were scanned at 5 μm resolution using GenePix Personal 4100A (Molecular Devices Corporation, Sunnyvale, CA).

    Article Title: Transcript Profiling Identifies Dynamic Gene Expression Patterns and an Important Role for Nrf2/Keap1 Pathway in the Developing Mouse Esophagus
    Article Snippet: Samples were moved to ice, and fragmentation was stopped by addition of 125 μl of Agilent's 2× in situ hybridization buffer. .. Microarrays were hybridized in Agilent Microarray Hybridization Chambers for 17 hours at 60°C with mixing on an Agilent Rotator in a Robbin's Scientific Hybridization Oven. .. After hybridization, the arrays were scanned by an Axon GenePix 4000B scanner (Axon Instruments; Foster City, CA).

    Article Title: Network-Based Gene Expression Biomarkers for Cold and Heat Patterns of Rheumatoid Arthritis in Traditional Chinese Medicine
    Article Snippet: .. The arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to the Agilent protocol. .. The arrays were scanned at a 5 μ m resolution using a Genepix 4000B scanner (Molecular Devices Corporation, Sunnyvale, CA).

    Article Title: Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival
    Article Snippet: .. This “hybridization sandwich” was assembled in an Agilent microarray hybridization chambers. ..

    Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes.
    Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Agilent microarray hybridization chambers with rotation at room temperature (RT). ..

    Article Title: Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats
    Article Snippet: All these steps were done using Gene expression hybridization kit (Agilent Technologies, Palo Alto, CA). .. 48 μl of these mixes were used for the hybridization, which was done in microarray hybridization chambers (Agilent Technologies, Palo Alto, CA). ..

    Hybridization:

    Article Title: Multi-Omics Analysis of Western-style Diet Increased Susceptibility to Experimental Colitis in Mice
    Article Snippet: The labeled RNAs were amplified and transcribed into cRNA using RNeasy Mini Kit (74104, Qiagen). .. The fluorescent cRNAs were hybridized onto the microarray hybridization chambers (G2530-60029, Agilent Technologies). .. After the microarray was washed, an Agilent DNA Microarray Scanner (G2565BA, Agilent Technologies) was used to scan the signals.

    Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression
    Article Snippet: .. Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008). .. Gene expression profiles were evaluated using Agilent whole rat genome 4×44K oligonucleotide microarrays.

    Article Title: Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets
    Article Snippet: Microarray hybridizations were carried out on labelled cRNAs with specificity greater than 8 using the in situ Hybridization Kit Plus (Agilent Technologies). .. Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to the Agilent protocol. .. Arrays were scanned at 5 μm resolution using GenePix Personal 4100A (Molecular Devices Corporation, Sunnyvale, CA).

    Article Title: Transcript Profiling Identifies Dynamic Gene Expression Patterns and an Important Role for Nrf2/Keap1 Pathway in the Developing Mouse Esophagus
    Article Snippet: Samples were moved to ice, and fragmentation was stopped by addition of 125 μl of Agilent's 2× in situ hybridization buffer. .. Microarrays were hybridized in Agilent Microarray Hybridization Chambers for 17 hours at 60°C with mixing on an Agilent Rotator in a Robbin's Scientific Hybridization Oven. .. After hybridization, the arrays were scanned by an Axon GenePix 4000B scanner (Axon Instruments; Foster City, CA).

    Article Title: Network-Based Gene Expression Biomarkers for Cold and Heat Patterns of Rheumatoid Arthritis in Traditional Chinese Medicine
    Article Snippet: .. The arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to the Agilent protocol. .. The arrays were scanned at a 5 μ m resolution using a Genepix 4000B scanner (Molecular Devices Corporation, Sunnyvale, CA).

    Article Title: Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival
    Article Snippet: .. This “hybridization sandwich” was assembled in an Agilent microarray hybridization chambers. ..

    Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes.
    Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Agilent microarray hybridization chambers with rotation at room temperature (RT). ..

    Article Title: Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats
    Article Snippet: All these steps were done using Gene expression hybridization kit (Agilent Technologies, Palo Alto, CA). .. 48 μl of these mixes were used for the hybridization, which was done in microarray hybridization chambers (Agilent Technologies, Palo Alto, CA). ..

    Incubation:

    Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression
    Article Snippet: .. Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008). .. Gene expression profiles were evaluated using Agilent whole rat genome 4×44K oligonucleotide microarrays.

    Article Title: Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets
    Article Snippet: Microarray hybridizations were carried out on labelled cRNAs with specificity greater than 8 using the in situ Hybridization Kit Plus (Agilent Technologies). .. Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to the Agilent protocol. .. Arrays were scanned at 5 μm resolution using GenePix Personal 4100A (Molecular Devices Corporation, Sunnyvale, CA).

    Article Title: Network-Based Gene Expression Biomarkers for Cold and Heat Patterns of Rheumatoid Arthritis in Traditional Chinese Medicine
    Article Snippet: .. The arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to the Agilent protocol. .. The arrays were scanned at a 5 μ m resolution using a Genepix 4000B scanner (Molecular Devices Corporation, Sunnyvale, CA).

    Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes.
    Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Agilent microarray hybridization chambers with rotation at room temperature (RT). ..

    Gene Expression:

    Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression
    Article Snippet: .. Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008). .. Gene expression profiles were evaluated using Agilent whole rat genome 4×44K oligonucleotide microarrays.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes.
    Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Agilent microarray hybridization chambers with rotation at room temperature (RT). ..

    Binding Assay:

    Article Title: Poly-ligand profiling differentiates trastuzumab-treated breast cancer patients according to their outcomes.
    Article Snippet: For positive selection, 400 μl of blocking buffer (0.8 ng/μl sheared salmon sperm DNA (Life Technologies), 0.8 ng/μl tRNA (Life Technologies), 1 μg/ μl HSA (Sigma), 0.5% F127 (Thermo Fisher) and 3 mM MgCl2 in 1× phosphatebuffered saline (PBS)) was mixed with 90 μl of ssDNA library solution (7 pmol in 1× PBS, 3 mM MgCl2) on top of the Agilent gasket slide. .. FFPE tissue slide, after deparaffinization and epitope retrieval, was mounted on top of the gasket slide containing binding cocktail and incubated for 1 h in Agilent microarray hybridization chambers with rotation at room temperature (RT). ..



    Similar Products

    90
    Agilent technologies microarray hybridization chamber kit
    Microarray Hybridization Chamber Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chambers/pmc10127108-156-15-19
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Camlab Ltd microarray hybridization chamber
    Microarray Hybridization Chamber, supplied by Camlab Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chambers/hybridization+chamber/pm40561749-99-7-10
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies microarray hybridization chamber
    Microarray Hybridization Chamber, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chambers/pm36768818-416-7-6
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Corning Life Sciences microarray hybridization chambers
    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
    Microarray Hybridization Chambers, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chambers/microarray+hybridization+chambers/pmc03733744-47-7-10
    Average 90 stars, based on 1 article reviews
    microarray hybridization chambers - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Corning Life Sciences microarray hybridization chamber
    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
    Microarray Hybridization Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chambers/microarray+hybridization+chambers/pmc01435976-68-34-40
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies microarray hybridization chambers
    A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide <t>microarray</t> data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.
    Microarray Hybridization Chambers, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chambers/pmc04217762-226-10-9
    Average 90 stars, based on 1 article reviews
    microarray hybridization chambers - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Journal: BMC Cancer

    Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

    doi: 10.1186/1471-2407-13-364

    Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing

    A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

    Journal: PLoS ONE

    Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression

    doi: 10.1371/journal.pone.0110934

    Figure Lengend Snippet: A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

    Article Snippet: Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008).

    Techniques: Expressing, Microarray, Derivative Assay