microarray hybridisation chamber user guide (Agilent technologies)

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Agilent technologies microarray hybridisation chamber user guide
Microarray Hybridisation Chamber User Guide, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation/us11976284-506-7-12
Average 90 stars, based on 1 article reviews

microarray hybridisation chamber user guide - by Bioz Stars, 2026-09

90/100 stars

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Hybridization:

Article Title: Profiling transcriptional heterogeneity with Seq-Well S 3 : A low-cost, portable, high-fidelity platform for massively-parallel single-cell RNA-seq
Article Snippet: 30 Glass Slides, precleaned (Thermo-Fisher Cat. No. 12–550-A3). .. 31 Hybridization Clamp10 (Agilent Cat. No. G2534A). ..

Article Title: Profiling transcriptional heterogeneity with Seq-Well S 3 : A low-cost, portable, high-fidelity platform for massively-parallel single-cell RNA-seq
Article Snippet: 30 30 Glass Slides, precleaned (Thermo-Fisher Cat. No. 12–550-A3). .. 31 31 Hybridization Clamp10 (Agilent Cat. No. G2534A). ..

Article Title: Whole Blood Transcriptomics in Cardiac Surgery Identifies a Gene Regulatory Network Connecting Ischemia Reperfusion with Systemic Inflammation
Article Snippet: Fragmentation was stopped by adding 55 μl 2x GEx hybridisation buffer HI-RPM leading to a final volume of 110 μl (Gene expression hybridization kit, Agilent #5188–5242). .. Chip hybridization was performed using the gasket/slide-sandwich system and the hybridisation chamber (Agilent, #G2534A) according to manufacturer instructions. .. During hybridisation slides were rotated at 10 rpm and 65°C for 16 h. Microarray slides were washed 1 min in GE Wash Buffer 1 (Agilent, #5188–5326) at room temperature, 1 min in GE Wash Buffer 2 (Agilent, #5188–5326) at room temperature and 30s in Acetonitril at room temperature on a magnetic stirrer.

Article Title: Promoter variants for protein production
Article Snippet: In-house designed P. pastoris specific oligonucleotide arrays (AMAD-ID: 026594, 8×15K custom arrays, Agilent) were used. .. Microarray hybridisation was done according to the Microarray Hybridisation Chamber User Guide (Agilent G2534A). .. First, the gasket slide was uncovered and put onto the chamber base, Agilent label facing up.

Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.

Microarray:

Article Title: Tisochrysis lutea F&M-M36 Mitigates Risk Factors of Metabolic Syndrome and Promotes Visceral Fat Browning through β3-Adrenergic Receptor/UCP1 Signaling
Article Snippet: Yields of cRNA and the dye incorporation rate were measured with the NanoPhotometer spectrophotometer (IMPLEN). .. Sample mixture was loaded into the Agilent Rat GE 8 × 60 K v2 Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65° for 18 h. Fluorescent signal intensities were detected using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using the Agilent Feature Extraction 9.5.3.1 software.

Article Title: miR-337-3p and Its Targets STAT3 and RAP1A Modulate Taxane Sensitivity in Non-Small Cell Lung Cancers
Article Snippet: For microRNA analysis, the samples were labeled using the miRNA Complete Labeling and Hyb Kit and hybridized to Agilent Human miRNA microarray version 3 chips (Agilent Technologies), which contains probes for 866 human and 89 human viral microRNAs based on miRBase v12.0 ( http://microrna.sanger.ac.uk ). .. Hybridizations were performed in stainless steel SureHyb chambers (G2534A) at 55°C for 22 h, after which arrays were washed and scanned using an Agilent DNA Microarray Scanner (Agilent Technologies). miRNA expression levels were extracted using the Feature Extraction software (Agilent Technologies) and processed with the bioconductor package AgiMicroRna to correct for background, remove control and un-detectable sequences, and quantile normalize and summarize the data. .. To examine the effect of miR-337-3p on paclitaxel sensitivity in lung cancer cells, we used miR-337-3p mimic (Dharmacon) to increase miR-337-3p levels in NCI-H1155 cells, a lung cancer cell line characterized as moderately resistant to paclitaxel in a previous study .

Control:

Software:

other:

Article Title: Systematic detection of m 6 A-modified transcripts at single-molecule and single-cell resolution.
Article Snippet: To lyse the loaded cells, the array was covered with 400 mL lysis buffer (20 mM Tris-HCl, pH8.0, 150 mM NaCl, 5 mM MgCl2, 1 mM EDTA, e5 Cell Reports Methods 1, 100061, September 27, 2021 0.2% NP-40, SUPERase$In RNase Inhibitor) and immediately sealed with the poly(dT50) coverslip using the manual clamp (Agilent, Cat. #G2534A).