microarray hybridisation (Arraystar inc)
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Microarray Hybridisation, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation/microarray+hybridization/pmc11461506-80-10-16
Average 90 stars, based on 1 article reviews
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1) Product Images from "FTO/IGF2BP2-mediated N6 methyladenosine modification in invasion and metastasis of thyroid carcinoma via CDH12"
Article Title: FTO/IGF2BP2-mediated N6 methyladenosine modification in invasion and metastasis of thyroid carcinoma via CDH12
Journal: Cell Death & Disease
doi: 10.1038/s41419-024-07097-4
Figure Legend Snippet: A M6A-mRNA epitranscriptomic microarray assay analysing the difference in m6A-related genes and mRNA levels between shFTO and control in CAL-62 cells ( n = 3 repetitions). B Overlapping of 1.5-fold m6A expression changes in CAL-62 cells with FTO knockdown and EMT-related functional genes. C Influences of shFTO or ( D ) FTO OE on the mRNA level of CDH12 in B-CPAP and CAL-62 cells ( n = 6 repetitions). E–F The influences of shFTO or FTO OE on the protein levels of CDH12 in B-CPAP and CAL-62 cells ( n = 3 repetitions). G Protein level of CDH12 in PTC and Nor tissues ( n = 7 paired). H The protein level of CDH12 in Nthy-ori 3-1, B-CPAP, CAL-62, and 8305 C cell lines ( n = 3 repetitions). I The mRNA level of CDH12 in PTC tissues and Nor tissues ( n = 40 paired). ( J ) Representative immunohistochemistry images of CDH12-positive cells in PTC and K ATC and Nor tissues (n = 5 paired). L MeRIP assay was performed to identify m6A antibody enrichment in CDH12 in CAL-62 cells ( n = 4 repetitions). M Variation in m6A modification enrichment of CDH12 after FTO knockdown in CAL-62 cells ( n = 4 repetitions). N Kaplan–Meier survival curves of disease-free survival based on CDH12. Bar=200 μm, * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Microarray, Control, Expressing, Knockdown, Functional Assay, Immunohistochemistry, Modification
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