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Arraystar inc microarray hybridisation
A M6A-mRNA epitranscriptomic <t>microarray</t> assay analysing the difference in m6A-related genes and mRNA levels between shFTO and control in CAL-62 cells ( n = 3 repetitions). B Overlapping of 1.5-fold m6A expression changes in CAL-62 cells with FTO knockdown and EMT-related functional genes. C Influences of shFTO or ( D ) FTO OE on the mRNA level of CDH12 in B-CPAP and CAL-62 cells ( n = 6 repetitions). E–F The influences of shFTO or FTO OE on the protein levels of CDH12 in B-CPAP and CAL-62 cells ( n = 3 repetitions). G Protein level of CDH12 in PTC and Nor tissues ( n = 7 paired). H The protein level of CDH12 in Nthy-ori 3-1, B-CPAP, CAL-62, and 8305 C cell lines ( n = 3 repetitions). I The mRNA level of CDH12 in PTC tissues and Nor tissues ( n = 40 paired). ( J ) Representative immunohistochemistry images of CDH12-positive cells in PTC and K ATC and Nor tissues (n = 5 paired). L MeRIP assay was performed to identify m6A antibody enrichment in CDH12 in CAL-62 cells ( n = 4 repetitions). M Variation in m6A modification enrichment of CDH12 after FTO knockdown in CAL-62 cells ( n = 4 repetitions). N Kaplan–Meier survival curves of disease-free survival based on CDH12. Bar=200 μm, * p < 0.05, ** p < 0.01, *** p < 0.001.
Microarray Hybridisation, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "FTO/IGF2BP2-mediated N6 methyladenosine modification in invasion and metastasis of thyroid carcinoma via CDH12"

Article Title: FTO/IGF2BP2-mediated N6 methyladenosine modification in invasion and metastasis of thyroid carcinoma via CDH12

Journal: Cell Death & Disease

doi: 10.1038/s41419-024-07097-4

A M6A-mRNA epitranscriptomic microarray assay analysing the difference in m6A-related genes and mRNA levels between shFTO and control in CAL-62 cells ( n = 3 repetitions). B Overlapping of 1.5-fold m6A expression changes in CAL-62 cells with FTO knockdown and EMT-related functional genes. C Influences of shFTO or ( D ) FTO OE on the mRNA level of CDH12 in B-CPAP and CAL-62 cells ( n = 6 repetitions). E–F The influences of shFTO or FTO OE on the protein levels of CDH12 in B-CPAP and CAL-62 cells ( n = 3 repetitions). G Protein level of CDH12 in PTC and Nor tissues ( n = 7 paired). H The protein level of CDH12 in Nthy-ori 3-1, B-CPAP, CAL-62, and 8305 C cell lines ( n = 3 repetitions). I The mRNA level of CDH12 in PTC tissues and Nor tissues ( n = 40 paired). ( J ) Representative immunohistochemistry images of CDH12-positive cells in PTC and K ATC and Nor tissues (n = 5 paired). L MeRIP assay was performed to identify m6A antibody enrichment in CDH12 in CAL-62 cells ( n = 4 repetitions). M Variation in m6A modification enrichment of CDH12 after FTO knockdown in CAL-62 cells ( n = 4 repetitions). N Kaplan–Meier survival curves of disease-free survival based on CDH12. Bar=200 μm, * p < 0.05, ** p < 0.01, *** p < 0.001.
Figure Legend Snippet: A M6A-mRNA epitranscriptomic microarray assay analysing the difference in m6A-related genes and mRNA levels between shFTO and control in CAL-62 cells ( n = 3 repetitions). B Overlapping of 1.5-fold m6A expression changes in CAL-62 cells with FTO knockdown and EMT-related functional genes. C Influences of shFTO or ( D ) FTO OE on the mRNA level of CDH12 in B-CPAP and CAL-62 cells ( n = 6 repetitions). E–F The influences of shFTO or FTO OE on the protein levels of CDH12 in B-CPAP and CAL-62 cells ( n = 3 repetitions). G Protein level of CDH12 in PTC and Nor tissues ( n = 7 paired). H The protein level of CDH12 in Nthy-ori 3-1, B-CPAP, CAL-62, and 8305 C cell lines ( n = 3 repetitions). I The mRNA level of CDH12 in PTC tissues and Nor tissues ( n = 40 paired). ( J ) Representative immunohistochemistry images of CDH12-positive cells in PTC and K ATC and Nor tissues (n = 5 paired). L MeRIP assay was performed to identify m6A antibody enrichment in CDH12 in CAL-62 cells ( n = 4 repetitions). M Variation in m6A modification enrichment of CDH12 after FTO knockdown in CAL-62 cells ( n = 4 repetitions). N Kaplan–Meier survival curves of disease-free survival based on CDH12. Bar=200 μm, * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques Used: Microarray, Control, Expressing, Knockdown, Functional Assay, Immunohistochemistry, Modification

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Sample Prep:

Article Title: Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer.
Article Snippet: .. All sample preparation and microarray hybridization were performed according to the protocols of Arraystar (Rockville, MD, USA). .. Briefly, circRNAs were enriched by removing linear RNAs with Rnase R. The enriched circRNAs were then amplified and transcribed into fluorescent cRNA with a random priming method (Arraystar Super RNA Labeling Kit).

Article Title: Circular RNA circTATDN3 promotes the Warburg effect and proliferation in colorectal cancer.
Article Snippet: As one of the key metabolic enzymes in the glycolytic pathway, lactate dehydrogenase A (LDHA) might be linked to tumor proliferation by driving the Warburg effect.. Circular RNAs (circRNAs) are widely implicated in tumor progression.. Here, we report that circTATDN3, a circular RNA that interacts with LDHA, plays a critical role in proliferation and energy metabolism in CRC.

Article Title: METTL3-m6A methylation inhibits the proliferation and viability of type II alveolar epithelial cells in acute lung injury by enhancing the stability and translation efficiency of Pten mRNA.
Article Snippet: The total RNA of each sample was quantified by the NanoDrop ND-1000 spectrophotometer, and the integrity of RNA was evaluated by the Bioanalyzer 2100 or Mops electrophoresis. .. Following Arraystar’s standard protocol, sample preparation and microarray hybridization were executed. ..

Article Title: CircXPO6 promotes breast cancer progression through competitively inhibiting the ubiquitination degradation of c-Myc.
Article Snippet: The number of breast cancer (BC) patients is increasing year by year, which is severely endangering to human life and health. c-Myc is a transcription factor, studies have shown that it is a very significant factor in tumor progression, but how it is regulated in BC is still not well understood.. Here, we used the RIP microarray sequencing to confirm circXPO6, which had a high affinity with c-Myc and highly expressed in triple-negative breast cancer (TNBC) tissues and cells.. CircXPO6 overexpression promoted tumor growth in vivo and in vitro.

Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

Article Title: METTL16 promotes corneal nerve regeneration in diabetic corneal neuropathy.
Article Snippet: As a leading ocular disorder caused by diabetes mellitus, diabetic corneal neuropathy (DCN) is characterized by decreased corneal sensation, ocular irritation, delayed corneal wound healing and corneal neuropathic pain.. Several researchers including us previously evidenced that DCN is regulated by several transcriptional and/or epigenetic factors.. However, the role of N6-methyladenosine (m6A) modification, the most common internal modification in eukaryotic RNAs, in the pathogenesis of DCN remains largely unexplored.

Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

Microarray:

Article Title: Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer.
Article Snippet: .. All sample preparation and microarray hybridization were performed according to the protocols of Arraystar (Rockville, MD, USA). .. Briefly, circRNAs were enriched by removing linear RNAs with Rnase R. The enriched circRNAs were then amplified and transcribed into fluorescent cRNA with a random priming method (Arraystar Super RNA Labeling Kit).

Article Title: Circular RNA circTATDN3 promotes the Warburg effect and proliferation in colorectal cancer.
Article Snippet: As one of the key metabolic enzymes in the glycolytic pathway, lactate dehydrogenase A (LDHA) might be linked to tumor proliferation by driving the Warburg effect.. Circular RNAs (circRNAs) are widely implicated in tumor progression.. Here, we report that circTATDN3, a circular RNA that interacts with LDHA, plays a critical role in proliferation and energy metabolism in CRC.

Article Title: METTL3-m6A methylation inhibits the proliferation and viability of type II alveolar epithelial cells in acute lung injury by enhancing the stability and translation efficiency of Pten mRNA.
Article Snippet: The total RNA of each sample was quantified by the NanoDrop ND-1000 spectrophotometer, and the integrity of RNA was evaluated by the Bioanalyzer 2100 or Mops electrophoresis. .. Following Arraystar’s standard protocol, sample preparation and microarray hybridization were executed. ..

Article Title: CircXPO6 promotes breast cancer progression through competitively inhibiting the ubiquitination degradation of c-Myc.
Article Snippet: The number of breast cancer (BC) patients is increasing year by year, which is severely endangering to human life and health. c-Myc is a transcription factor, studies have shown that it is a very significant factor in tumor progression, but how it is regulated in BC is still not well understood.. Here, we used the RIP microarray sequencing to confirm circXPO6, which had a high affinity with c-Myc and highly expressed in triple-negative breast cancer (TNBC) tissues and cells.. CircXPO6 overexpression promoted tumor growth in vivo and in vitro.

Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

Article Title: METTL16 promotes corneal nerve regeneration in diabetic corneal neuropathy.
Article Snippet: As a leading ocular disorder caused by diabetes mellitus, diabetic corneal neuropathy (DCN) is characterized by decreased corneal sensation, ocular irritation, delayed corneal wound healing and corneal neuropathic pain.. Several researchers including us previously evidenced that DCN is regulated by several transcriptional and/or epigenetic factors.. However, the role of N6-methyladenosine (m6A) modification, the most common internal modification in eukaryotic RNAs, in the pathogenesis of DCN remains largely unexplored.

Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

Hybridization:

Article Title: Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer.
Article Snippet: .. All sample preparation and microarray hybridization were performed according to the protocols of Arraystar (Rockville, MD, USA). .. Briefly, circRNAs were enriched by removing linear RNAs with Rnase R. The enriched circRNAs were then amplified and transcribed into fluorescent cRNA with a random priming method (Arraystar Super RNA Labeling Kit).

Article Title: Circular RNA circTATDN3 promotes the Warburg effect and proliferation in colorectal cancer.
Article Snippet: As one of the key metabolic enzymes in the glycolytic pathway, lactate dehydrogenase A (LDHA) might be linked to tumor proliferation by driving the Warburg effect.. Circular RNAs (circRNAs) are widely implicated in tumor progression.. Here, we report that circTATDN3, a circular RNA that interacts with LDHA, plays a critical role in proliferation and energy metabolism in CRC.

Article Title: METTL3-m6A methylation inhibits the proliferation and viability of type II alveolar epithelial cells in acute lung injury by enhancing the stability and translation efficiency of Pten mRNA.
Article Snippet: The total RNA of each sample was quantified by the NanoDrop ND-1000 spectrophotometer, and the integrity of RNA was evaluated by the Bioanalyzer 2100 or Mops electrophoresis. .. Following Arraystar’s standard protocol, sample preparation and microarray hybridization were executed. ..

Article Title: CircXPO6 promotes breast cancer progression through competitively inhibiting the ubiquitination degradation of c-Myc.
Article Snippet: The number of breast cancer (BC) patients is increasing year by year, which is severely endangering to human life and health. c-Myc is a transcription factor, studies have shown that it is a very significant factor in tumor progression, but how it is regulated in BC is still not well understood.. Here, we used the RIP microarray sequencing to confirm circXPO6, which had a high affinity with c-Myc and highly expressed in triple-negative breast cancer (TNBC) tissues and cells.. CircXPO6 overexpression promoted tumor growth in vivo and in vitro.

Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.

Article Title: METTL16 promotes corneal nerve regeneration in diabetic corneal neuropathy.
Article Snippet: As a leading ocular disorder caused by diabetes mellitus, diabetic corneal neuropathy (DCN) is characterized by decreased corneal sensation, ocular irritation, delayed corneal wound healing and corneal neuropathic pain.. Several researchers including us previously evidenced that DCN is regulated by several transcriptional and/or epigenetic factors.. However, the role of N6-methyladenosine (m6A) modification, the most common internal modification in eukaryotic RNAs, in the pathogenesis of DCN remains largely unexplored.

Article Title: m6A demethylase Fto inhibited macrophage activation and glycolysis in diabetic nephropathy via m6A/Npas2/Hif‐1α axis
Article Snippet: .. The sample preparation and microarray hybridization were conducted following the standard protocols provided by Arraystar. .. Acquired array images were analyzed using Agilent Feature Extraction software.



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Image Search Results


Scheme used to select probes for the Group I subtyping microarray Notes: A total of 225 probes were selected. 146 probes corresponding to strain variable regions of the ATCC 3502 genome sequence were selected based on CGH analysis using Group I types A, B, and F test strains. In silico analysis was used to select the remaining probes representing genes not present in ATCC 3502, specific for either OkraB or LanglelandF strains, or other specific genes of interest.

Journal: The botulinum journal

Article Title: Exploring genomic diversity in Clostridium botulinum using DNA microarrays

doi: 10.1504/tbj.2012.050195

Figure Lengend Snippet: Scheme used to select probes for the Group I subtyping microarray Notes: A total of 225 probes were selected. 146 probes corresponding to strain variable regions of the ATCC 3502 genome sequence were selected based on CGH analysis using Group I types A, B, and F test strains. In silico analysis was used to select the remaining probes representing genes not present in ATCC 3502, specific for either OkraB or LanglelandF strains, or other specific genes of interest.

Article Snippet: High density CGH microarrays Our laboratory developed a comparative genomic hybridisation (CGH) microarray featuring a total of 384,771 overlapping probes (~50–70 bp) representing the ATCC 3502 type A genome sequence in order to evaluate the level of genomic diversity among C. botulinum strains ( Raphael et al., 2008 ).

Techniques: Microarray, Sequencing, In Silico

Matrix of correlation coefficients of the  hybridisation  results for bont/F5 encoding strains using the Group I subtyping  microarray  (see online version for colours)

Journal: The botulinum journal

Article Title: Exploring genomic diversity in Clostridium botulinum using DNA microarrays

doi: 10.1504/tbj.2012.050195

Figure Lengend Snippet: Matrix of correlation coefficients of the hybridisation results for bont/F5 encoding strains using the Group I subtyping microarray (see online version for colours)

Article Snippet: High density CGH microarrays Our laboratory developed a comparative genomic hybridisation (CGH) microarray featuring a total of 384,771 overlapping probes (~50–70 bp) representing the ATCC 3502 type A genome sequence in order to evaluate the level of genomic diversity among C. botulinum strains ( Raphael et al., 2008 ).

Techniques: Hybridization, Microarray

Hybridisation of the Group II subtyping microarray by type E (n = 15), type B (n = 4) and type F (n = 3) C. botulinum strains Notes: Shown is the average % of probes hybridised (i.e., where log10 of the ratio of probe fluorescent compared to background ≥ 1.0) for each toxin serotype indicated. Error bars depict standard deviations.

Journal: The botulinum journal

Article Title: Exploring genomic diversity in Clostridium botulinum using DNA microarrays

doi: 10.1504/tbj.2012.050195

Figure Lengend Snippet: Hybridisation of the Group II subtyping microarray by type E (n = 15), type B (n = 4) and type F (n = 3) C. botulinum strains Notes: Shown is the average % of probes hybridised (i.e., where log10 of the ratio of probe fluorescent compared to background ≥ 1.0) for each toxin serotype indicated. Error bars depict standard deviations.

Article Snippet: High density CGH microarrays Our laboratory developed a comparative genomic hybridisation (CGH) microarray featuring a total of 384,771 overlapping probes (~50–70 bp) representing the ATCC 3502 type A genome sequence in order to evaluate the level of genomic diversity among C. botulinum strains ( Raphael et al., 2008 ).

Techniques: Hybridization, Microarray

A M6A-mRNA epitranscriptomic microarray assay analysing the difference in m6A-related genes and mRNA levels between shFTO and control in CAL-62 cells ( n = 3 repetitions). B Overlapping of 1.5-fold m6A expression changes in CAL-62 cells with FTO knockdown and EMT-related functional genes. C Influences of shFTO or ( D ) FTO OE on the mRNA level of CDH12 in B-CPAP and CAL-62 cells ( n = 6 repetitions). E–F The influences of shFTO or FTO OE on the protein levels of CDH12 in B-CPAP and CAL-62 cells ( n = 3 repetitions). G Protein level of CDH12 in PTC and Nor tissues ( n = 7 paired). H The protein level of CDH12 in Nthy-ori 3-1, B-CPAP, CAL-62, and 8305 C cell lines ( n = 3 repetitions). I The mRNA level of CDH12 in PTC tissues and Nor tissues ( n = 40 paired). ( J ) Representative immunohistochemistry images of CDH12-positive cells in PTC and K ATC and Nor tissues (n = 5 paired). L MeRIP assay was performed to identify m6A antibody enrichment in CDH12 in CAL-62 cells ( n = 4 repetitions). M Variation in m6A modification enrichment of CDH12 after FTO knockdown in CAL-62 cells ( n = 4 repetitions). N Kaplan–Meier survival curves of disease-free survival based on CDH12. Bar=200 μm, * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Cell Death & Disease

Article Title: FTO/IGF2BP2-mediated N6 methyladenosine modification in invasion and metastasis of thyroid carcinoma via CDH12

doi: 10.1038/s41419-024-07097-4

Figure Lengend Snippet: A M6A-mRNA epitranscriptomic microarray assay analysing the difference in m6A-related genes and mRNA levels between shFTO and control in CAL-62 cells ( n = 3 repetitions). B Overlapping of 1.5-fold m6A expression changes in CAL-62 cells with FTO knockdown and EMT-related functional genes. C Influences of shFTO or ( D ) FTO OE on the mRNA level of CDH12 in B-CPAP and CAL-62 cells ( n = 6 repetitions). E–F The influences of shFTO or FTO OE on the protein levels of CDH12 in B-CPAP and CAL-62 cells ( n = 3 repetitions). G Protein level of CDH12 in PTC and Nor tissues ( n = 7 paired). H The protein level of CDH12 in Nthy-ori 3-1, B-CPAP, CAL-62, and 8305 C cell lines ( n = 3 repetitions). I The mRNA level of CDH12 in PTC tissues and Nor tissues ( n = 40 paired). ( J ) Representative immunohistochemistry images of CDH12-positive cells in PTC and K ATC and Nor tissues (n = 5 paired). L MeRIP assay was performed to identify m6A antibody enrichment in CDH12 in CAL-62 cells ( n = 4 repetitions). M Variation in m6A modification enrichment of CDH12 after FTO knockdown in CAL-62 cells ( n = 4 repetitions). N Kaplan–Meier survival curves of disease-free survival based on CDH12. Bar=200 μm, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Total RNA ( n = 3 repetitions) was prepared and microarray hybridisation was performed according to Arraystar’s standard protocols.

Techniques: Microarray, Control, Expressing, Knockdown, Functional Assay, Immunohistochemistry, Modification

Fold changes obtained by microarray (fill) and by qPCR (hatched) when fed the M diet (dark grey) or the V diet (light grey) for genes involved in sensory perception (R23h versus A22h), immunity (R23h versus AB1h) and for amino acid metabolism (R23h versus A22h).

Journal: PLoS ONE

Article Title: Detection of new pathways involved in the acceptance and the utilisation of a plant-based diet in isogenic lines of rainbow trout fry

doi: 10.1371/journal.pone.0201462

Figure Lengend Snippet: Fold changes obtained by microarray (fill) and by qPCR (hatched) when fed the M diet (dark grey) or the V diet (light grey) for genes involved in sensory perception (R23h versus A22h), immunity (R23h versus AB1h) and for amino acid metabolism (R23h versus A22h).

Article Snippet: Hybridisation was performed in a microarray hybridisation oven (Agilent) for 17h at 65°C.

Techniques: Microarray

Correlation between gene expression patterns obtained through real-time PCR and  microarray  approaches.

Journal: PLoS ONE

Article Title: Detection of new pathways involved in the acceptance and the utilisation of a plant-based diet in isogenic lines of rainbow trout fry

doi: 10.1371/journal.pone.0201462

Figure Lengend Snippet: Correlation between gene expression patterns obtained through real-time PCR and microarray approaches.

Article Snippet: Hybridisation was performed in a microarray hybridisation oven (Agilent) for 17h at 65°C.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Microarray