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multiple sequence alignment feature of the dnastar lasergene® software  (DNASTAR)


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    DNASTAR multiple sequence alignment feature of the dnastar lasergene® software
    Multiple Sequence Alignment Feature Of The Dnastar Lasergene® Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lasergene+sequence+alignment+software/sequence+analysis+software/pmc02586281-64-47-47
    Average 90 stars, based on 1 article reviews
    multiple sequence alignment feature of the dnastar lasergene® software - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice
    Article Snippet: The PCR products were purified using the QIAQuick PCR purification kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and sent for Sanger sequencing at the KFSH&RC sequencing facility. .. The potential off-target sequences were analysed and lack of mutations within potential off-target sequences were confirmed using DNASTAR sequence analysis software package (DNASTAR, Inc., WI, USA). ..

    Article Title: Characterization of the complete mitochondrial genome of Ergasilus anchoratus Markevich, 1946 (Ergasilidae) and phylogeny of Copepoda.
    Article Snippet: The mitochondrial (mt) genome can provide data for phylogenetic analyses and evolutionary biology.. Herein, we sequenced and annotated the complete mt genome of Ergasilus anchoratus.. This mt genome was 13852 bp long and comprised 13 protein-coding genes (PCGs), 22 tRNAs and 2 rRNAs.

    Article Title: C-MET agonistic antibody and use thereof
    Article Snippet: .. Sequence alignment and percent identity calculation can be performed using a sequence analysis software (e.g., the Megalign program in the LASERGENE Bioinformatics Calculation Suite (DNASTAR Inc., Madison, Wis.)). .. In the pharmaceutical composition of the present invention, the antibody is comprised in a therapeutically effective amount.

    Article Title: Genomic characterization and pathogenicity of a novel fowl adenovirus serotype 11 isolated from chickens with inclusion body hepatitis in China
    Article Snippet: The amplicons were purified and sent to Sangon Biotech (Shanghai) Co., Ltd., for sequencing. .. The complete genome sequence was assembled using the Seqman program of the Lasergene sequence analysis software package (DNASTAR, Inc., Madison, WI) and deposited in GenBank. ..

    Article Title: Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.
    Article Snippet: Funding information US Public Health Service, Grant/Award Numbers: DE025225, DE027850, AI105362; EgyptianMinistry of Education Abstract Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease.. Additionally, our group has recently demonstrated that Treponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma.. While over 60 species and phylotypes of oral Treponema have been detected, T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed.

    Article Title: The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice
    Article Snippet: PCR products were purified using the QIAQuick PCR purification kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and subject to Sanger sequencing. .. The sequences were analysed and compared to the WT Plcζ sequence using DNASTAR sequence analysis software package (DNASTAR, Inc., WI, USA). ..

    Article Title: Decoding seasonal changes: soil parameters and microbial communities in tropical dry deciduous forests
    Article Snippet: The sequencing reaction employed the Big Dye Terminator Cycle Sequencing Kit V3.1 (Applied Biosystems, United States) following the manufacturer’s protocol. .. The resulting sequence chromatograms were analyzed using DNASTAR Lasergene sequence analysis software , to generate sequences for molecular identification. ..

    Software:

    Article Title: The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice
    Article Snippet: The PCR products were purified using the QIAQuick PCR purification kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and sent for Sanger sequencing at the KFSH&RC sequencing facility. .. The potential off-target sequences were analysed and lack of mutations within potential off-target sequences were confirmed using DNASTAR sequence analysis software package (DNASTAR, Inc., WI, USA). ..

    Article Title: Characterization of the complete mitochondrial genome of Ergasilus anchoratus Markevich, 1946 (Ergasilidae) and phylogeny of Copepoda.
    Article Snippet: The mitochondrial (mt) genome can provide data for phylogenetic analyses and evolutionary biology.. Herein, we sequenced and annotated the complete mt genome of Ergasilus anchoratus.. This mt genome was 13852 bp long and comprised 13 protein-coding genes (PCGs), 22 tRNAs and 2 rRNAs.

    Article Title: C-MET agonistic antibody and use thereof
    Article Snippet: .. Sequence alignment and percent identity calculation can be performed using a sequence analysis software (e.g., the Megalign program in the LASERGENE Bioinformatics Calculation Suite (DNASTAR Inc., Madison, Wis.)). .. In the pharmaceutical composition of the present invention, the antibody is comprised in a therapeutically effective amount.

    Article Title: Genomic characterization and pathogenicity of a novel fowl adenovirus serotype 11 isolated from chickens with inclusion body hepatitis in China
    Article Snippet: The amplicons were purified and sent to Sangon Biotech (Shanghai) Co., Ltd., for sequencing. .. The complete genome sequence was assembled using the Seqman program of the Lasergene sequence analysis software package (DNASTAR, Inc., Madison, WI) and deposited in GenBank. ..

    Article Title: Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.
    Article Snippet: Funding information US Public Health Service, Grant/Award Numbers: DE025225, DE027850, AI105362; EgyptianMinistry of Education Abstract Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease.. Additionally, our group has recently demonstrated that Treponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma.. While over 60 species and phylotypes of oral Treponema have been detected, T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed.

    Article Title: The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice
    Article Snippet: PCR products were purified using the QIAQuick PCR purification kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and subject to Sanger sequencing. .. The sequences were analysed and compared to the WT Plcζ sequence using DNASTAR sequence analysis software package (DNASTAR, Inc., WI, USA). ..

    Article Title: Decoding seasonal changes: soil parameters and microbial communities in tropical dry deciduous forests
    Article Snippet: The sequencing reaction employed the Big Dye Terminator Cycle Sequencing Kit V3.1 (Applied Biosystems, United States) following the manufacturer’s protocol. .. The resulting sequence chromatograms were analyzed using DNASTAR Lasergene sequence analysis software , to generate sequences for molecular identification. ..

    other:

    Article Title: Impact of Baloxavir Resistance-Associated Substitutions on Influenza Virus Growth and Drug Susceptibility.
    Article Snippet: Baloxavir marboxil (baloxavir) is a recently FDA-approved influenza virus polymerase acidic (PA) endonuclease inhibitor.. Several PA substitutions have been demonstrated to confer reduced susceptibility to baloxavir; however, their impacts on measurements of antiviral drug susceptibility and replication capacity when present as a fraction of the viral population have not been established.. We generated recombinant A/California/04/ 09 (H1N1)-like viruses (IAV) with PA I38L, I38T, or E199D substitutions and B/Victoria/504/ 2000-like virus (IBV) with PA I38T.

    Recombinant:

    Article Title: Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.
    Article Snippet: Funding information US Public Health Service, Grant/Award Numbers: DE025225, DE027850, AI105362; EgyptianMinistry of Education Abstract Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease.. Additionally, our group has recently demonstrated that Treponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma.. While over 60 species and phylotypes of oral Treponema have been detected, T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed.

    Plasmid Preparation:

    Article Title: Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.
    Article Snippet: Funding information US Public Health Service, Grant/Award Numbers: DE025225, DE027850, AI105362; EgyptianMinistry of Education Abstract Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease.. Additionally, our group has recently demonstrated that Treponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma.. While over 60 species and phylotypes of oral Treponema have been detected, T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed.

    Construct:

    Article Title: Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.
    Article Snippet: Funding information US Public Health Service, Grant/Award Numbers: DE025225, DE027850, AI105362; EgyptianMinistry of Education Abstract Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease.. Additionally, our group has recently demonstrated that Treponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma.. While over 60 species and phylotypes of oral Treponema have been detected, T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed.

    DNA Sequencing:

    Article Title: Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.
    Article Snippet: Funding information US Public Health Service, Grant/Award Numbers: DE025225, DE027850, AI105362; EgyptianMinistry of Education Abstract Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease.. Additionally, our group has recently demonstrated that Treponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma.. While over 60 species and phylotypes of oral Treponema have been detected, T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed.



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    Image Search Results


    Table of Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Defining Gene Functions in Tumorigenesis by Ex Vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

    doi: 10.3791/62740

    Figure Lengend Snippet: Table of Materials

    Article Snippet: For the bioluminescence (MTT) assays, perform the assay exactly as outlined in the manufacturer’s instructions every 24 h for 72 h using a luminometer plate reader. table ft1 table-wrap mode="anchored" t5 caption a7 Name of Material/ Equipment Company Catalog Number Comments Ad5CMV-eGFP Gene Transfer Vector Core, Univ of Iowa VVC-U of Iowa-4 Ad5CMVCre-eGFP Gene Transfer Vector Core, Univ of Iowa VVC-U of Iowa-1174 alexa 568 secondary antibody Thermo/Fisher GαR A11036 Bioconductor Open Source Software for Bioninformatics Bioconductor http://www.bioconductor.org alternative statistical analysis tool used for step 4.4 CD31 Abcam ab28364 Celigo Image Cytometer Nexcelom Bioscience N/A Cell Stripper Corning 25-056-Cl mixture of chelators DAB staining kit Vector Labs MP-7800 DAVID ( D atabase for A nnotation, V isualization, and I ntegrated D iscovery) DAVID https://david.ncifcrf.gov functional enrichment analysis software used for step 4.5 DMEM Corning 15-013-Cl DreamTaq and Buffer (Genotyping PCR) Thermo/Fisher EP0701 and K1072 erbB4 antibodies Santa Cruz sc-284 erbB4 antibodies Abcam ab35374 erbB4 antibodies Millipore HFR1: 05-1133 FACS Sorter BD Biosciences Aria II Forskolin Sigma F6886 GenomeSpace Tools and Data Sources GenomeSpace https://genomespace.org/support/tools/ general resource for several types of open source bioinformatic tools for step 4.5 Glutamine Corning 25-005-Cl Gorilla G ene Ontology en RI chment ana L ysis and visua L iz A tion tool Gorilla N/A, http://cbl-gorilla.cs.technion.ac.il functional enrichment analysis software used for step 4.5 GSEA Gene Set Enrichment Analysis Broad Institute N/A, https://www.gsea-msigdb.org/gsea/index.jsp functional enrichment analysis software used for step 4.5 HSD: Athymic Nude-FOxn1nu mice Envigo (Previously Harlan Labs) 69 Illumina HiSeq2500 (next generation DNA sequencer) Illumina Hi Seq 2500 DNA sequencer used for step 4.2 Lasergene: ArrayStar Gene expression and variant analysis DNAStar LaserGene software N/A software statistical and normalization analysis used for step 4.4 Lasergene: SeqMan NGen sequence alignment assembly software alignment used for step 4.3 N/A software alignment used for step 4.3 Matrigel, low growth factor basement membrane matrix Corning 354230 NRG1-beta In house Generated by SLC, also commercially available from R & D Systems(396-HB-050/CF).

    Techniques: Plasmid Preparation, Software, Cytometry, Staining, Functional Assay, Expressing, Variant Assay, Sequencing, Generated, Viability Assay, Amplification