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lasergene sequence alignment software  (DNASTAR)


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    Structured Review

    DNASTAR lasergene sequence alignment software
    Lasergene Sequence Alignment Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 10848 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lasergene+sequence+alignment+software/pmc06450159-68-33-36
    Average 99 stars, based on 10848 article reviews
    lasergene sequence alignment software - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Software:

    Article Title: First Isolation, Genomic Characterization and Experimental Infection of Bovine Coronavirus from Sheep
    Article Snippet: PCR products were examined by agarose gel electrophoresis, purified using a PCR purification kit (Tiangen Biotech Co., Ltd., Beijing, China; Cat. No. DP214-02), cloned into the pMD19-T vector (Takara, Japan; Cat. No. 6013), and subjected to Sanger sequencing by Chengdu Qinke Biotechnology Co., Ltd. (Chengdu, China). .. The Sangerderived sequences were aligned with the metagenomic assemblies using SeqMan software in the DNASTAR Lasergene package version 7.1 (DNASTAR Inc., Madison, WI, USA). ..

    Article Title: Restoration of infectivity-virulence and toxin biosynthesis in Botrytis cinerea B459 via upstream-targeted marker-free CRISPR/Cas9 editing of the transcription factor Bcin04g03490.
    Article Snippet: For sgRNA purification, RNA Clean and Concentrator 25 (ref.Rl O18; Zymo Research; USA) was used. .. Sequence analysis utilized software from the DNASTAR Lasergene package (DNASTAR; USA). .. Oligonucleotides (Table S1) were sourced from Metabion (Metabion International AG; Germany).

    Article Title: Co-circulation of H5N1 and H5N6 high pathogenicity avian influenza viruses in migratory birds at a coastal wintering site in China.
    Article Snippet: The amplified products were sent to Comate Bioscience Co., Ltd. (Changchun, China) for Sanger sequencing. .. Viral gene segments were analysed using the DNAStar Lasergene software package. ..

    Article Title: Immunogenicity of recombinant porcine parainfluenza virus 5 expressing the spike protein of porcine epidemic diarrhea virus.
    Article Snippet: The porcine epidemic diarrhea virus (PEDV) induces severe diarrhea in neonatal piglets, often resulting in high mortality rates.. Consequently, the development of effective and safe vaccines is a critical priority for controlling PEDV infection.. Vaccines vectored by the parainfluenza virus 5 (PIV5) exhibit distinct advantages in terms of safety, genomic stability, and immunogenicity.

    Article Title: Otx2 directly regulates tbx3 expression during early eye formation.
    Article Snippet: After identification of 10k base pairs upstream and downstream the tbx3 gene for each species in NCBI:Gene or Ensembl.org, ECR were identified using ECR Browser (http://ecrbrowser.dcode.org) and MultiPIPMaker, as previously described (Ogino et al., 2006; Ledford et al., 2017). .. All sequence analysis was performed using the Lasergene Software Package (DNASTAR, Madison, WI). .. Putative transcription factor binding sites were identified using rVista (rvista.dcode.org) with a TRANSFAC professional v10.2 library.

    Article Title: Structural analyses of a heterodimeric SusCD complex captures intermediate states of maltooligosaccharide transport.
    Article Snippet: .. Burland, T. G. (2000) DNASTAR's Lasergene sequence analysis software. ..

    Article Title: Chitinase production and effects of land use change on fungal communities in the soil of the Brazilian Cerrado.
    Article Snippet: The resulting amplicons were subjected to Sanger sequencing at Instituto René Rachou (FIOCRUZ Minas). .. Sanger sequencing chromatograms were processed using DNASTAR Lasergene software to generate consensus ITS sequences. ..

    Sequencing:

    Article Title: Restoration of infectivity-virulence and toxin biosynthesis in Botrytis cinerea B459 via upstream-targeted marker-free CRISPR/Cas9 editing of the transcription factor Bcin04g03490.
    Article Snippet: For sgRNA purification, RNA Clean and Concentrator 25 (ref.Rl O18; Zymo Research; USA) was used. .. Sequence analysis utilized software from the DNASTAR Lasergene package (DNASTAR; USA). .. Oligonucleotides (Table S1) were sourced from Metabion (Metabion International AG; Germany).

    Article Title: Immunogenicity of recombinant porcine parainfluenza virus 5 expressing the spike protein of porcine epidemic diarrhea virus.
    Article Snippet: The porcine epidemic diarrhea virus (PEDV) induces severe diarrhea in neonatal piglets, often resulting in high mortality rates.. Consequently, the development of effective and safe vaccines is a critical priority for controlling PEDV infection.. Vaccines vectored by the parainfluenza virus 5 (PIV5) exhibit distinct advantages in terms of safety, genomic stability, and immunogenicity.

    Article Title: Otx2 directly regulates tbx3 expression during early eye formation.
    Article Snippet: After identification of 10k base pairs upstream and downstream the tbx3 gene for each species in NCBI:Gene or Ensembl.org, ECR were identified using ECR Browser (http://ecrbrowser.dcode.org) and MultiPIPMaker, as previously described (Ogino et al., 2006; Ledford et al., 2017). .. All sequence analysis was performed using the Lasergene Software Package (DNASTAR, Madison, WI). .. Putative transcription factor binding sites were identified using rVista (rvista.dcode.org) with a TRANSFAC professional v10.2 library.

    Article Title: Structural analyses of a heterodimeric SusCD complex captures intermediate states of maltooligosaccharide transport.
    Article Snippet: .. Burland, T. G. (2000) DNASTAR's Lasergene sequence analysis software. ..

    Analysis:

    Article Title: Restoration of infectivity-virulence and toxin biosynthesis in Botrytis cinerea B459 via upstream-targeted marker-free CRISPR/Cas9 editing of the transcription factor Bcin04g03490.
    Article Snippet: For sgRNA purification, RNA Clean and Concentrator 25 (ref.Rl O18; Zymo Research; USA) was used. .. Sequence analysis utilized software from the DNASTAR Lasergene package (DNASTAR; USA). .. Oligonucleotides (Table S1) were sourced from Metabion (Metabion International AG; Germany).

    Article Title: Otx2 directly regulates tbx3 expression during early eye formation.
    Article Snippet: After identification of 10k base pairs upstream and downstream the tbx3 gene for each species in NCBI:Gene or Ensembl.org, ECR were identified using ECR Browser (http://ecrbrowser.dcode.org) and MultiPIPMaker, as previously described (Ogino et al., 2006; Ledford et al., 2017). .. All sequence analysis was performed using the Lasergene Software Package (DNASTAR, Madison, WI). .. Putative transcription factor binding sites were identified using rVista (rvista.dcode.org) with a TRANSFAC professional v10.2 library.

    Article Title: Structural analyses of a heterodimeric SusCD complex captures intermediate states of maltooligosaccharide transport.
    Article Snippet: .. Burland, T. G. (2000) DNASTAR's Lasergene sequence analysis software. ..

    Hemagglutination Assay:

    Article Title: H9N2 avian influenza virus in Muscovy ducks: a nationwide epidemiological survey and vaccine efficacy assessment.
    Article Snippet: For molecular identification, reverse transcription (RT)-PCR was performed to amplify the HA genes via specific primers (HA-F: 5′- CAA GAT GGA AGT AGT ATC ACT-3′; HA-R: 5′- TTG CCA ATT ATA TAC AAA TGT − 3′), and the resulting PCR products were sequenced by Shanghai Sangon Biotech. .. Nucleotide sequences of the HA gene were assembled via DNAstar Lasergene 11. ..

    DNA Sequencing:

    Article Title: Chitinase production and effects of land use change on fungal communities in the soil of the Brazilian Cerrado.
    Article Snippet: The resulting amplicons were subjected to Sanger sequencing at Instituto René Rachou (FIOCRUZ Minas). .. Sanger sequencing chromatograms were processed using DNASTAR Lasergene software to generate consensus ITS sequences. ..



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    Table of Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Defining Gene Functions in Tumorigenesis by Ex Vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

    doi: 10.3791/62740

    Figure Lengend Snippet: Table of Materials

    Article Snippet: For the bioluminescence (MTT) assays, perform the assay exactly as outlined in the manufacturer’s instructions every 24 h for 72 h using a luminometer plate reader. table ft1 table-wrap mode="anchored" t5 caption a7 Name of Material/ Equipment Company Catalog Number Comments Ad5CMV-eGFP Gene Transfer Vector Core, Univ of Iowa VVC-U of Iowa-4 Ad5CMVCre-eGFP Gene Transfer Vector Core, Univ of Iowa VVC-U of Iowa-1174 alexa 568 secondary antibody Thermo/Fisher GαR A11036 Bioconductor Open Source Software for Bioninformatics Bioconductor http://www.bioconductor.org alternative statistical analysis tool used for step 4.4 CD31 Abcam ab28364 Celigo Image Cytometer Nexcelom Bioscience N/A Cell Stripper Corning 25-056-Cl mixture of chelators DAB staining kit Vector Labs MP-7800 DAVID ( D atabase for A nnotation, V isualization, and I ntegrated D iscovery) DAVID https://david.ncifcrf.gov functional enrichment analysis software used for step 4.5 DMEM Corning 15-013-Cl DreamTaq and Buffer (Genotyping PCR) Thermo/Fisher EP0701 and K1072 erbB4 antibodies Santa Cruz sc-284 erbB4 antibodies Abcam ab35374 erbB4 antibodies Millipore HFR1: 05-1133 FACS Sorter BD Biosciences Aria II Forskolin Sigma F6886 GenomeSpace Tools and Data Sources GenomeSpace https://genomespace.org/support/tools/ general resource for several types of open source bioinformatic tools for step 4.5 Glutamine Corning 25-005-Cl Gorilla G ene Ontology en RI chment ana L ysis and visua L iz A tion tool Gorilla N/A, http://cbl-gorilla.cs.technion.ac.il functional enrichment analysis software used for step 4.5 GSEA Gene Set Enrichment Analysis Broad Institute N/A, https://www.gsea-msigdb.org/gsea/index.jsp functional enrichment analysis software used for step 4.5 HSD: Athymic Nude-FOxn1nu mice Envigo (Previously Harlan Labs) 69 Illumina HiSeq2500 (next generation DNA sequencer) Illumina Hi Seq 2500 DNA sequencer used for step 4.2 Lasergene: ArrayStar Gene expression and variant analysis DNAStar LaserGene software N/A software statistical and normalization analysis used for step 4.4 Lasergene: SeqMan NGen sequence alignment assembly software alignment used for step 4.3 N/A software alignment used for step 4.3 Matrigel, low growth factor basement membrane matrix Corning 354230 NRG1-beta In house Generated by SLC, also commercially available from R & D Systems(396-HB-050/CF).

    Techniques: Plasmid Preparation, Software, Cytometry, Staining, Functional Assay, Expressing, Variant Assay, Sequencing, Generated, Viability Assay, Amplification