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n6951s  (New England Biolabs)


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    Structured Review

    New England Biolabs n6951s

    N6951s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+vector+dna/pTWIN1+Vector+DNA/pmc07065910-6-11-7
    Average 93 stars, based on 223 article reviews
    n6951s - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Human RPA activates BLM’s bidirectional DNA unwinding from a nick"

    Article Title: Human RPA activates BLM’s bidirectional DNA unwinding from a nick

    Journal: eLife

    doi: 10.7554/eLife.54098


    Figure Legend Snippet:

    Techniques Used: Recombinant, Lambda DNA Preparation, Plasmid Preparation, Expressing, DNA Purification, Staining, Software

    Related Articles

    Plasmid Preparation:

    Article Title: Streamlined protein expression and purification using cleavable self-aggregating tags
    Article Snippet: The pfu DNA polymerase was from Tiangen (Beijing, China). .. The vector pTWIN1 was from New England Biolabs. .. The vector pET30a (+) and strain E. coli BL21 (DE3) were from Novagen (Madison, WI).

    Article Title: Phosphorylation and O-GlcNAcylation at the same α-synuclein site generate distinct fibril structures
    Article Snippet: .. To obtain segment X, α-syn 1-84 was introduced into pTWIN1 vector (NEB) containing intein and chitin binding domain. ..

    Polymerase Chain Reaction:

    Article Title: Synthesis of L-nucleic acids by means of an enzyme
    Article Snippet: .. PCR product 2 was made using pTWIN1 (New England Biolabs, Frankfurt am Main, Germany) as a template and primers MJ_1_72_DD (5′-Phosphate-AGCGGCTCTTCGTGCATCACGGGAGAT-3′, (SEQ ID: 66)) and MJ_1_73_DD (5′-Phosphate-AGCGGCTCTTCGCCCTTGAAGCTGCCACAAGGCAGGAACGTT-3′, (SEQ ID: 67)) to amplify the Mxe Gyr A intein and the CBD. .. PCR product 2 was made using pTWIN1 (New England Biolabs, Frankfurt am Main, Germany) as a template and primers MJ_1_72_DD (5′-Phosphate-AGCGGCTCTTCGTGCATCACGGGAGAT-3′, (SEQ ID: 66)) and MJ_1_73_DD (5′-Phosphate-AGCGGCTCTTCGCCCTTGAAGCTGCCACAAGGCAGGAACGTT-3′, (SEQ ID: 67)) to amplify the Mxe Gyr A intein and the CBD.

    Article Title: Recycling of hyoscyamine 6β-hydroxylase for the in vitro production of anisodamine and scopolamine.
    Article Snippet: The tropane alkaloids hyoscyamine, anisodamine, and scopolamine are extensively used medicines.. In particular, scopolamine has the greatest value in the market.. Hence, strategies to enhance its production have been explored as an alternative to traditional field-plant cultivation.

    Article Title: Synthesis of L-nucleic acids by means of an enzyme
    Article Snippet: .. PCR product 2 was made using pTWIN1 (New England Biolabs, Frankfurt am Main, Germany) as a template and primers MJ_1_72_DD (5′-Phosphate-AGCGGCTCTTCGTGCATCACGGGAGAT-3′, (SEQ ID NO: 66)) and MJ_1_73_DD (5′-Phosphate-AGCGGCTCTTCGCCCTTGAAGCTGCCACAAGGCAGGAACGTT-3′, (SEQ ID NO: 67)) to amplify the Mxe Gyr A intein and the CBD. .. PCR product 2 was made using pTWIN1 (New England Biolabs, Frankfurt am Main, Germany) as a template and primers MJ_1_72_DD (5′-Phosphate-AGCGGCTCTTCGTGCATCACGGGAGAT-3′, (SEQ ID NO: 66)) and MJ_1_73_DD (5′-Phosphate-AGCGGCTCTTCGCCCTTGAAGCTGCCACAAGGCAGGAACGTT-3′, (SEQ ID NO: 67)) to amplify the Mxe Gyr A intein and the CBD.

    Binding Assay:

    Article Title: Phosphorylation and O-GlcNAcylation at the same α-synuclein site generate distinct fibril structures
    Article Snippet: .. To obtain segment X, α-syn 1-84 was introduced into pTWIN1 vector (NEB) containing intein and chitin binding domain. ..

    Article Title: Cooperative action of separate interaction domains promotes high-affinity DNA binding of Arabidopsis thaliana ARF transcription factors.
    Article Snippet: .. Briefly, the genomic regions corresponding to the DNA binding domain (DBD) of A. thaliana ARF1, ARF2 ARF5, and full-length ARF2 were amplified and cloned in a modified expression vector pTWIN1 (New England Biolabs) to generate fusions with the Chitin Binding Domain (CBD) and Intein. .. ARF–CBD fusion proteins were expressed in Escherichia coli strain Rosetta DE3 (Novagen).

    Amplification:

    Article Title: Cooperative action of separate interaction domains promotes high-affinity DNA binding of Arabidopsis thaliana ARF transcription factors.
    Article Snippet: .. Briefly, the genomic regions corresponding to the DNA binding domain (DBD) of A. thaliana ARF1, ARF2 ARF5, and full-length ARF2 were amplified and cloned in a modified expression vector pTWIN1 (New England Biolabs) to generate fusions with the Chitin Binding Domain (CBD) and Intein. .. ARF–CBD fusion proteins were expressed in Escherichia coli strain Rosetta DE3 (Novagen).

    Clone Assay:

    Article Title: Cooperative action of separate interaction domains promotes high-affinity DNA binding of Arabidopsis thaliana ARF transcription factors.
    Article Snippet: .. Briefly, the genomic regions corresponding to the DNA binding domain (DBD) of A. thaliana ARF1, ARF2 ARF5, and full-length ARF2 were amplified and cloned in a modified expression vector pTWIN1 (New England Biolabs) to generate fusions with the Chitin Binding Domain (CBD) and Intein. .. ARF–CBD fusion proteins were expressed in Escherichia coli strain Rosetta DE3 (Novagen).

    Modification:

    Article Title: Cooperative action of separate interaction domains promotes high-affinity DNA binding of Arabidopsis thaliana ARF transcription factors.
    Article Snippet: .. Briefly, the genomic regions corresponding to the DNA binding domain (DBD) of A. thaliana ARF1, ARF2 ARF5, and full-length ARF2 were amplified and cloned in a modified expression vector pTWIN1 (New England Biolabs) to generate fusions with the Chitin Binding Domain (CBD) and Intein. .. ARF–CBD fusion proteins were expressed in Escherichia coli strain Rosetta DE3 (Novagen).

    Expressing:

    Article Title: Cooperative action of separate interaction domains promotes high-affinity DNA binding of Arabidopsis thaliana ARF transcription factors.
    Article Snippet: .. Briefly, the genomic regions corresponding to the DNA binding domain (DBD) of A. thaliana ARF1, ARF2 ARF5, and full-length ARF2 were amplified and cloned in a modified expression vector pTWIN1 (New England Biolabs) to generate fusions with the Chitin Binding Domain (CBD) and Intein. .. ARF–CBD fusion proteins were expressed in Escherichia coli strain Rosetta DE3 (Novagen).

    Protease Inhibitor:

    Article Title: Recycling of hyoscyamine 6β-hydroxylase for the in vitro production of anisodamine and scopolamine.
    Article Snippet: The tropane alkaloids hyoscyamine, anisodamine, and scopolamine are extensively used medicines.. In particular, scopolamine has the greatest value in the market.. Hence, strategies to enhance its production have been explored as an alternative to traditional field-plant cultivation.



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    Image Search Results


    Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

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    Figure Lengend Snippet: Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

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    Techniques: Binding Assay, Incubation, Imaging, Lambda DNA Preparation, Plasmid Preparation, Isolation