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phage lambda dna  (New England Biolabs)


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    New England Biolabs phage lambda dna
    Eap binding to <t>DNA</t> is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage <t>lambda</t> DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.
    Phage Lambda Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+vector+dna/pBR322+Vector/pmc06047304-38-4-20
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    Images

    1) Product Images from "The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation"

    Article Title: The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2018.00235

    Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.
    Figure Legend Snippet: Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Techniques Used: Binding Assay, Incubation, Imaging, Lambda DNA Preparation, Plasmid Preparation, Isolation

    Related Articles

    Plasmid Preparation:

    Article Title: Nickel(II) coordination compounds of 4'-chloro-fenamic acid: characterization and biological activity.
    Article Snippet: .. They were purchased from commercial sources: NiCl2·6H2O, Himi, py, βpic, neoc, phen, bipyam, KOH, K2CO3, CuSO4, CT DNA, BSA, HSA, EB and ABTS from Sigma Aldrich Co; DPPH, nordihydroguaiaretic acid (NDGA), butylated hydroxytoluene (BHT), K2S2O8, and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox) from J&K Scientific; H2O2 (30% w/v) from PanReac AppliChem ITW Reagents; sodium warfarin, ibuprofen, NaCl and trisodium citrate from Tokyo Chemical Industry (TCI); supercoiled circular pBR322 plasmid DNA from New England Biolabs; 2-chlorobenzoic acid, and 4-chloroaniline from Alfa Aesar; L-ascorbic acid, Na2HPO4, NaH2PO4, HCl (35% v/v) and solvents from Chemlab Co. CT DNA was diluted with buffer (15 mM trisodium citrate and 150 mM NaCl at pH 7.0) and was stirred to form the CT DNA stock solution. ..

    Article Title: Bio-Orthogonal Chemistry-Based Strategy to Turn-OFF CRISPR-Cas9 Activity in Solution and Live Cells
    Article Snippet: The resulting TCO-modified CRISPR suppressors, TCO-CPP-RRWQW, TCO-CPP-RLRWR, AAA-PNA-TCO and TTT-PNA-TCO were purified by preparative HPLC using Phalanx C18 250x20 mm column (Higgins Analytical, cat. # PS-2520-C185). .. CRISPR-Cas9 in vitro DNA cleavage assay: pBR322 plasmid is a 4,361 base pairs long dsDNA (New England Biolabs, 1 mg/mL, cat.# N3033S). ..

    Article Title: Method of treating or ameliorating metabolic disorders using antagonistic binding proteins for gastric inhibitory peptide receptor (GIPR)/GLP-1 receptor agonist fusion proteins
    Article Snippet: .. For example, the origin of replication from the plasmid pBR322 (New England Biolabs, Beverly, MA) is suitable for most gram-negative bacteria, and various viral origins (e.g., SV40, polyoma, adenovirus, vesicular stomatitus virus (VSV), or papillomaviruses such as HPV or BPV) are useful for cloning vectors in mammalian cells. ..

    Article Title: Bio-orthogonal chemistry-based strategy to Turn-OFF CRISPR-Cas9 activity in solution and live cells
    Article Snippet: The resulting TCO-modified CRISPR suppressors, TCO-CPP-RRWQW, TCO-CPP-RLRWR, AAA-PNA-TCO , and TTT-PNA-TCO were purified by preparative HPLC using Phalanx C18 250x20 mm column (Higgins Analytical, cat. # PS-2520-C185). .. pBR322 plasmid is a 4 361 base pairs long dsDNA (New England Biolabs, 1 mg/mL, cat.# N3033S). .. 1 μL of pBR322 plasmid DNA was diluted with water (16.87 μL) and NEBuffer TM 3.1 (10x, 2 μL).

    Recombinant:

    Article Title: HPF1 regulates the formation of FUS-dependent compartments by PARP1 and PARP2 activation on damaged DNA.
    Article Snippet: .. Recombinant DNA was used in this study Recombinant DNA Sourse Identifier Vector pBR322 New England BioLabs CatN3033S pET-FUS Singatulina et al ., 2019 [ 32 ] N/A pET-FUS- LCD Singatulina et al ., 2019 [ 32 ] N/A pET-FUS_1 –374 Singatulina et al ., 2019 [ 32 ] N/A pET-FUS_1 –454 Singatulina et al ., 2019 [ 32 ] N/A pET-MBP-FUS_FL_6E Monahan et al ., 2017 [ 48 ] Addgene #98652 pET-MBP-FUS_FL_12E Monahan et al ., 2017 [ 48 ] Addgene #98655 pLate31-HPF1-His Kurgina et al ., 2021 [ 14 ] N/A pGEX-2T-bPARG-GST Dr V. Schreiber N/A pXC53-hAPE1 Dr S.H. .. Recombinant DNA was used in this study Recombinant DNA Sourse Identifier Vector pBR322 New England BioLabs Cat#N3033S pET-FUS Singatulina et al ., 2019 [ 32 ] N/A pET-FUS- LCD Singatulina et al ., 2019 [ 32 ] N/A pET-FUS_1 –374 Singatulina et al ., 2019 [ 32 ] N/A pET-FUS_1 –454 Singatulina et al ., 2019 [ 32 ] N/A pET-MBP-FUS_FL_6E Monahan et al ., 2017 [ 48 ] Addgene #98652 pET-MBP-FUS_FL_12E Monahan et al ., 2017 [ 48 ] Addgene #98655 pLate31-HPF1-His Kurgina et al ., 2021 [ 14 ] N/A pGEX-2T-bPARG-GST Dr V. Schreiber N/A pXC53-hAPE1 Dr S.H.

    Marker:

    Article Title: Regulated synthetic gene expression systems
    Article Snippet: .. The tetracycline (tet) and ampicillin (amp) resistance marker genes can be obtained from any of a number of commercially available vectors including pBR322 (available from New England BioLabs, Beverly, Mass., cat. no. 303-3s). ..

    other:

    Article Title: Acetate promotes nutritional adaptation in Escherichia coli
    Article Snippet: The other plasmids were constructed by Gibson assembly (NEBuilder HiFi DNA Assembly from New England Biolab, France) performed following the protocol recommended by the manufacturer to combine a plasmidic chassis and double reporter system from the pSB1C3 plasmid.

    CRISPR:

    Article Title: Bio-Orthogonal Chemistry-Based Strategy to Turn-OFF CRISPR-Cas9 Activity in Solution and Live Cells
    Article Snippet: The resulting TCO-modified CRISPR suppressors, TCO-CPP-RRWQW, TCO-CPP-RLRWR, AAA-PNA-TCO and TTT-PNA-TCO were purified by preparative HPLC using Phalanx C18 250x20 mm column (Higgins Analytical, cat. # PS-2520-C185). .. CRISPR-Cas9 in vitro DNA cleavage assay: pBR322 plasmid is a 4,361 base pairs long dsDNA (New England Biolabs, 1 mg/mL, cat.# N3033S). ..

    In Vitro:

    Article Title: Bio-Orthogonal Chemistry-Based Strategy to Turn-OFF CRISPR-Cas9 Activity in Solution and Live Cells
    Article Snippet: The resulting TCO-modified CRISPR suppressors, TCO-CPP-RRWQW, TCO-CPP-RLRWR, AAA-PNA-TCO and TTT-PNA-TCO were purified by preparative HPLC using Phalanx C18 250x20 mm column (Higgins Analytical, cat. # PS-2520-C185). .. CRISPR-Cas9 in vitro DNA cleavage assay: pBR322 plasmid is a 4,361 base pairs long dsDNA (New England Biolabs, 1 mg/mL, cat.# N3033S). ..

    DNA Cleavage Assay:

    Article Title: Bio-Orthogonal Chemistry-Based Strategy to Turn-OFF CRISPR-Cas9 Activity in Solution and Live Cells
    Article Snippet: The resulting TCO-modified CRISPR suppressors, TCO-CPP-RRWQW, TCO-CPP-RLRWR, AAA-PNA-TCO and TTT-PNA-TCO were purified by preparative HPLC using Phalanx C18 250x20 mm column (Higgins Analytical, cat. # PS-2520-C185). .. CRISPR-Cas9 in vitro DNA cleavage assay: pBR322 plasmid is a 4,361 base pairs long dsDNA (New England Biolabs, 1 mg/mL, cat.# N3033S). ..

    Bacteria:

    Article Title: Method of treating or ameliorating metabolic disorders using antagonistic binding proteins for gastric inhibitory peptide receptor (GIPR)/GLP-1 receptor agonist fusion proteins
    Article Snippet: .. For example, the origin of replication from the plasmid pBR322 (New England Biolabs, Beverly, MA) is suitable for most gram-negative bacteria, and various viral origins (e.g., SV40, polyoma, adenovirus, vesicular stomatitus virus (VSV), or papillomaviruses such as HPV or BPV) are useful for cloning vectors in mammalian cells. ..

    Virus:

    Article Title: Method of treating or ameliorating metabolic disorders using antagonistic binding proteins for gastric inhibitory peptide receptor (GIPR)/GLP-1 receptor agonist fusion proteins
    Article Snippet: .. For example, the origin of replication from the plasmid pBR322 (New England Biolabs, Beverly, MA) is suitable for most gram-negative bacteria, and various viral origins (e.g., SV40, polyoma, adenovirus, vesicular stomatitus virus (VSV), or papillomaviruses such as HPV or BPV) are useful for cloning vectors in mammalian cells. ..

    Cloning:

    Article Title: Method of treating or ameliorating metabolic disorders using antagonistic binding proteins for gastric inhibitory peptide receptor (GIPR)/GLP-1 receptor agonist fusion proteins
    Article Snippet: .. For example, the origin of replication from the plasmid pBR322 (New England Biolabs, Beverly, MA) is suitable for most gram-negative bacteria, and various viral origins (e.g., SV40, polyoma, adenovirus, vesicular stomatitus virus (VSV), or papillomaviruses such as HPV or BPV) are useful for cloning vectors in mammalian cells. ..



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    Image Search Results


    Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

    doi: 10.3389/fcimb.2018.00235

    Figure Lengend Snippet: Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Article Snippet: Herring sperm DNA (10164142), phage lambda DNA (D3779), and pBR322 (N3033L) DNA were purchased from Invitrogen (Karlsruhe, Germany), Sigma-Aldrich, and New England Biolabs (Frankfurt, Germany), respectively.

    Techniques: Binding Assay, Incubation, Imaging, Lambda DNA Preparation, Plasmid Preparation, Isolation