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lambda zap ii vector dna  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda zap ii vector dna
    Lambda Zap Ii Vector Dna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+vector+dna/us06844178-155-16-23
    Average 90 stars, based on 1 article reviews
    lambda zap ii vector dna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Recombinant kid pregastric esterase and methods for its production and use
    Article Snippet: .. The resulting double-stranded DNA molecules were then cut with Eco R1 restriction enzyme and ligated into Eco R1-cut Lambda ZAP II vector DNA (Stratagene Cloning Systems, La Jolla, Calif.) to produce a library of Lambda ZAP II DNAs, each of which presumably contained one cDNA derived from one mRNA that was present in kid lingual tissue mRNA population. .. The library of cDNA-containing Lambda ZAP II DNAs was then packaged to form virulent bacteriophage using a commercially prepared packaging extract (Gigapack II gold packaging extract, Stratagene Cloning Systems, La Jolla, Calif.) and used to infect an appropriate strain of bacteria (XL1-Blue, Stratagene Cloning Systems, La Jolla, Calif.).

    Cloning:

    Article Title: Recombinant kid pregastric esterase and methods for its production and use
    Article Snippet: .. The resulting double-stranded DNA molecules were then cut with Eco R1 restriction enzyme and ligated into Eco R1-cut Lambda ZAP II vector DNA (Stratagene Cloning Systems, La Jolla, Calif.) to produce a library of Lambda ZAP II DNAs, each of which presumably contained one cDNA derived from one mRNA that was present in kid lingual tissue mRNA population. .. The library of cDNA-containing Lambda ZAP II DNAs was then packaged to form virulent bacteriophage using a commercially prepared packaging extract (Gigapack II gold packaging extract, Stratagene Cloning Systems, La Jolla, Calif.) and used to infect an appropriate strain of bacteria (XL1-Blue, Stratagene Cloning Systems, La Jolla, Calif.).

    Derivative Assay:

    Article Title: Recombinant kid pregastric esterase and methods for its production and use
    Article Snippet: .. The resulting double-stranded DNA molecules were then cut with Eco R1 restriction enzyme and ligated into Eco R1-cut Lambda ZAP II vector DNA (Stratagene Cloning Systems, La Jolla, Calif.) to produce a library of Lambda ZAP II DNAs, each of which presumably contained one cDNA derived from one mRNA that was present in kid lingual tissue mRNA population. .. The library of cDNA-containing Lambda ZAP II DNAs was then packaged to form virulent bacteriophage using a commercially prepared packaging extract (Gigapack II gold packaging extract, Stratagene Cloning Systems, La Jolla, Calif.) and used to infect an appropriate strain of bacteria (XL1-Blue, Stratagene Cloning Systems, La Jolla, Calif.).



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    Image Search Results


    Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

    doi: 10.3389/fcimb.2018.00235

    Figure Lengend Snippet: Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Article Snippet: Herring sperm DNA (10164142), phage lambda DNA (D3779), and pBR322 (N3033L) DNA were purchased from Invitrogen (Karlsruhe, Germany), Sigma-Aldrich, and New England Biolabs (Frankfurt, Germany), respectively.

    Techniques: Binding Assay, Incubation, Imaging, Lambda DNA Preparation, Plasmid Preparation, Isolation