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lambda phage fix ii vector system  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda phage fix ii vector system
    Lambda Phage Fix Ii Vector System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+fix+ii/pmc04825118-318-7-26
    Average 90 stars, based on 1 article reviews
    lambda phage fix ii vector system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Construct:

    Article Title: PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules
    Article Snippet: .. A genomic library constructed with a DNA from the A10 strain (55) in the lambda phage vector FIX II (Stratagene) was screened by using a fragment of cloned sequence flanking the insertion site in the mutant B4 as a probe. ..

    Article Title: New enzyme lineages by subdomain shuffling
    Article Snippet: .. The DNA fragment encoding rtrypsinogen was amplified by PCR on human pancreas cDNA constructed in the phage vector Lambda ZAP II (Stratagene) by using the oligonucleotides 5′-AAAAAACCATGGATGATGATGACAAGATCGTTGGG-3′ and 5′AAAAAAAAGCTTCATTAGCTATTGGCAGCTATGGTGTTC-3′ and cloned into rfX-expression plasmid. ..

    Article Title: Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+CD38- cells: possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells.
    Article Snippet: .. For library cloning, amplified differentially expressed cDNA was cleaved within the KV sequence with EcoRI, purified, and ligated into EcoRI-digested, dephosphorylated lambda ZAP II phage vector (Stratagene, La Jolla, CA, USA) to construct a phage library according to the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules
    Article Snippet: .. A genomic library constructed with a DNA from the A10 strain (55) in the lambda phage vector FIX II (Stratagene) was screened by using a fragment of cloned sequence flanking the insertion site in the mutant B4 as a probe. ..

    Article Title: A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions
    Article Snippet: .. The in vivo excision properties of the Lambda phage FIX II vector system allowed the recovery of selected clones in the pBluescript SK plasmid for sequencing (Agilent Technologies). ..

    Article Title: Genomic organization of the JEM-1 (BLZF1) gene on human chromosome 1q24: molecular cloning and analysis of its promoter region.
    Article Snippet: n The Jem-1 (JEM-1, HGMW-approved symbol BLZF1) gene mapping to human chromosome 1q24 codes for a ubiquitously expressed 3-kb mRNA, translated in a 45-kDa nuclear protein.. Recent studies have shown a deficient expression of this gene in acute promyelocytic leukemia (APL).. However, treatment with retinoids was able to upregulate JEM-1 mRNA in maturing NB4 leukemia cells.

    Article Title: New enzyme lineages by subdomain shuffling
    Article Snippet: .. The DNA fragment encoding rtrypsinogen was amplified by PCR on human pancreas cDNA constructed in the phage vector Lambda ZAP II (Stratagene) by using the oligonucleotides 5′-AAAAAACCATGGATGATGATGACAAGATCGTTGGG-3′ and 5′AAAAAAAAGCTTCATTAGCTATTGGCAGCTATGGTGTTC-3′ and cloned into rfX-expression plasmid. ..

    Article Title: Zebrafish caspase-3: molecular cloning, characterization, crystallization and phylogenetic analysis.
    Article Snippet: Apoptosis plays an important role in maintaining the normal function of various tissues and organs in different species.. Caspase-3 is a terminal caspases which plays an important role in the execution of apoptosis in all vertebrates.. It was cloned from zebra fish embryos and its properties were identified through Western blotting and biological activity.

    Article Title: Properties of the P-Type ATPases Encoded by the copAP Operons of Helicobacter pylori and Helicobacter felis
    Article Snippet: .. Methylated DNA was cloned into the Eco RI cloning site of the Lambda ZAP II phage vector (Stratagene), providing the ability to excise the pBluescript SK(−) phagemid by using the M13 helper phage (Stratagene). ..

    Article Title: Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+CD38- cells: possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells.
    Article Snippet: .. For library cloning, amplified differentially expressed cDNA was cleaved within the KV sequence with EcoRI, purified, and ligated into EcoRI-digested, dephosphorylated lambda ZAP II phage vector (Stratagene, La Jolla, CA, USA) to construct a phage library according to the manufacturer’s protocol. ..

    Clone Assay:

    Article Title: PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules
    Article Snippet: .. A genomic library constructed with a DNA from the A10 strain (55) in the lambda phage vector FIX II (Stratagene) was screened by using a fragment of cloned sequence flanking the insertion site in the mutant B4 as a probe. ..

    Article Title: A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions
    Article Snippet: .. The in vivo excision properties of the Lambda phage FIX II vector system allowed the recovery of selected clones in the pBluescript SK plasmid for sequencing (Agilent Technologies). ..

    Article Title: New enzyme lineages by subdomain shuffling
    Article Snippet: .. The DNA fragment encoding rtrypsinogen was amplified by PCR on human pancreas cDNA constructed in the phage vector Lambda ZAP II (Stratagene) by using the oligonucleotides 5′-AAAAAACCATGGATGATGATGACAAGATCGTTGGG-3′ and 5′AAAAAAAAGCTTCATTAGCTATTGGCAGCTATGGTGTTC-3′ and cloned into rfX-expression plasmid. ..

    Article Title: Zebrafish caspase-3: molecular cloning, characterization, crystallization and phylogenetic analysis.
    Article Snippet: Apoptosis plays an important role in maintaining the normal function of various tissues and organs in different species.. Caspase-3 is a terminal caspases which plays an important role in the execution of apoptosis in all vertebrates.. It was cloned from zebra fish embryos and its properties were identified through Western blotting and biological activity.

    Article Title: Properties of the P-Type ATPases Encoded by the copAP Operons of Helicobacter pylori and Helicobacter felis
    Article Snippet: .. Methylated DNA was cloned into the Eco RI cloning site of the Lambda ZAP II phage vector (Stratagene), providing the ability to excise the pBluescript SK(−) phagemid by using the M13 helper phage (Stratagene). ..

    Sequencing:

    Article Title: PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules
    Article Snippet: .. A genomic library constructed with a DNA from the A10 strain (55) in the lambda phage vector FIX II (Stratagene) was screened by using a fragment of cloned sequence flanking the insertion site in the mutant B4 as a probe. ..

    Article Title: A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions
    Article Snippet: .. The in vivo excision properties of the Lambda phage FIX II vector system allowed the recovery of selected clones in the pBluescript SK plasmid for sequencing (Agilent Technologies). ..

    Article Title: Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+CD38- cells: possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells.
    Article Snippet: .. For library cloning, amplified differentially expressed cDNA was cleaved within the KV sequence with EcoRI, purified, and ligated into EcoRI-digested, dephosphorylated lambda ZAP II phage vector (Stratagene, La Jolla, CA, USA) to construct a phage library according to the manufacturer’s protocol. ..

    Mutagenesis:

    Article Title: PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules
    Article Snippet: .. A genomic library constructed with a DNA from the A10 strain (55) in the lambda phage vector FIX II (Stratagene) was screened by using a fragment of cloned sequence flanking the insertion site in the mutant B4 as a probe. ..

    In Vivo:

    Article Title: A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions
    Article Snippet: .. The in vivo excision properties of the Lambda phage FIX II vector system allowed the recovery of selected clones in the pBluescript SK plasmid for sequencing (Agilent Technologies). ..

    Hybridization:

    Article Title: Genomic organization of the JEM-1 (BLZF1) gene on human chromosome 1q24: molecular cloning and analysis of its promoter region.
    Article Snippet: n The Jem-1 (JEM-1, HGMW-approved symbol BLZF1) gene mapping to human chromosome 1q24 codes for a ubiquitously expressed 3-kb mRNA, translated in a 45-kDa nuclear protein.. Recent studies have shown a deficient expression of this gene in acute promyelocytic leukemia (APL).. However, treatment with retinoids was able to upregulate JEM-1 mRNA in maturing NB4 leukemia cells.

    Article Title: Zebrafish caspase-3: molecular cloning, characterization, crystallization and phylogenetic analysis.
    Article Snippet: Apoptosis plays an important role in maintaining the normal function of various tissues and organs in different species.. Caspase-3 is a terminal caspases which plays an important role in the execution of apoptosis in all vertebrates.. It was cloned from zebra fish embryos and its properties were identified through Western blotting and biological activity.

    Amplification:

    Article Title: New enzyme lineages by subdomain shuffling
    Article Snippet: .. The DNA fragment encoding rtrypsinogen was amplified by PCR on human pancreas cDNA constructed in the phage vector Lambda ZAP II (Stratagene) by using the oligonucleotides 5′-AAAAAACCATGGATGATGATGACAAGATCGTTGGG-3′ and 5′AAAAAAAAGCTTCATTAGCTATTGGCAGCTATGGTGTTC-3′ and cloned into rfX-expression plasmid. ..

    Article Title: Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+CD38- cells: possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells.
    Article Snippet: .. For library cloning, amplified differentially expressed cDNA was cleaved within the KV sequence with EcoRI, purified, and ligated into EcoRI-digested, dephosphorylated lambda ZAP II phage vector (Stratagene, La Jolla, CA, USA) to construct a phage library according to the manufacturer’s protocol. ..

    Polymerase Chain Reaction:

    Article Title: New enzyme lineages by subdomain shuffling
    Article Snippet: .. The DNA fragment encoding rtrypsinogen was amplified by PCR on human pancreas cDNA constructed in the phage vector Lambda ZAP II (Stratagene) by using the oligonucleotides 5′-AAAAAACCATGGATGATGATGACAAGATCGTTGGG-3′ and 5′AAAAAAAAGCTTCATTAGCTATTGGCAGCTATGGTGTTC-3′ and cloned into rfX-expression plasmid. ..

    Control:

    Article Title: Immediate-early genes and synaptic function.
    Article Snippet: Classical studies have demonstrated a role for protein synthesis in long-term memory.. The focus of our research is to identify the proteins that are essential for memory and to discover how they contribute to activity-dependent neuronal plasticity.. We have developed whole-animal models that maximize the induction of activity-dependent genes and have used differential cloning techniques to identify a set of novel, neuronal immediate-early genes (IEGs).

    cDNA Library Assay:

    Article Title: Zebrafish caspase-3: molecular cloning, characterization, crystallization and phylogenetic analysis.
    Article Snippet: Apoptosis plays an important role in maintaining the normal function of various tissues and organs in different species.. Caspase-3 is a terminal caspases which plays an important role in the execution of apoptosis in all vertebrates.. It was cloned from zebra fish embryos and its properties were identified through Western blotting and biological activity.

    Labeling:

    Article Title: Zebrafish caspase-3: molecular cloning, characterization, crystallization and phylogenetic analysis.
    Article Snippet: Apoptosis plays an important role in maintaining the normal function of various tissues and organs in different species.. Caspase-3 is a terminal caspases which plays an important role in the execution of apoptosis in all vertebrates.. It was cloned from zebra fish embryos and its properties were identified through Western blotting and biological activity.

    Methylation:

    Article Title: Properties of the P-Type ATPases Encoded by the copAP Operons of Helicobacter pylori and Helicobacter felis
    Article Snippet: .. Methylated DNA was cloned into the Eco RI cloning site of the Lambda ZAP II phage vector (Stratagene), providing the ability to excise the pBluescript SK(−) phagemid by using the M13 helper phage (Stratagene). ..

    Cloning:

    Article Title: Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+CD38- cells: possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells.
    Article Snippet: .. For library cloning, amplified differentially expressed cDNA was cleaved within the KV sequence with EcoRI, purified, and ligated into EcoRI-digested, dephosphorylated lambda ZAP II phage vector (Stratagene, La Jolla, CA, USA) to construct a phage library according to the manufacturer’s protocol. ..

    Purification:

    Article Title: Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+CD38- cells: possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells.
    Article Snippet: .. For library cloning, amplified differentially expressed cDNA was cleaved within the KV sequence with EcoRI, purified, and ligated into EcoRI-digested, dephosphorylated lambda ZAP II phage vector (Stratagene, La Jolla, CA, USA) to construct a phage library according to the manufacturer’s protocol. ..



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