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lambda fix ii  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda fix ii
    Lambda Fix Ii, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+fix+ii/pm27374149-34-9-12
    Average 90 stars, based on 1 article reviews
    lambda fix ii - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Construct:

    Article Title: Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole-genome duplication.
    Article Snippet: .. An Atlantic salmon genomic DNA library was constructed in lambda FIX II (Stratagene, USA, Zheng et al., 2009). ..

    Article Title: The molecular logic for planarian regeneration along the anterior-posterior axis.
    Article Snippet: The planarian Dugesia japonica can regenerate a complete individual from a head, trunk or tail fragment via activation of somatic pluripotent stem cells.. About a century ago, Thomas Hunt Morgan attempted to explain the extraordinary regenerative ability of planarians by positing two opposing morphogenetic gradients of formative ‘‘head stuff’’ and ‘‘tail stuff’’ along the anterior– posterior axis.. However, Morgan’s hypothesis remains open to debate.

    Article Title: Investigating hookworm genomes by comparative analysis of two Ancylostoma species
    Article Snippet: Poly (A)+ RNA was isolated from total RNA using the Oligotex mRNA isolation kit (Qiagen, Chatsworth, CA). .. Approximately 5 μg of mRNA was used to construct a directional cDNA library in Lambda ZAP II (Stratagene, La Jolla, CA) as previously described [ ]. pBluescript phagemid were mass excised prior to sequencing. ..

    Article Title: Piriform Spider Silk Sequences Reveal Unique Repetitive Elements
    Article Snippet: Due to the difficulty in separating aciniform from piriform glands, libraries were constructed from mRNA isolated from piriform-enriched posterior regions. mRNA extraction Fifty to 100mg of individual glands or posterior regions were pooled and homogenized in TRI reagent and total RNA was extracted according to the manufacturer’s protocol (Molecular Research Center, Cincinnati, OH). mRNA was isolated using Dynabeads oligo(dT) 25 (Dynal, Inc, Lake Success, NY) for N. clavipes and A. trifasciata or by the Oligotex kit (Qiagen, Germany) for N. cruentata . .. The mRNA concentration was determined spectrophotometrically. cDNA Library Construction cDNA from N. clavipes and A. trifasciata was synthesized with Superscript II Choice System (Invitrogen, Carlsbad, CA) and Lambda ZAP II libraries (Stratagene, La Jolla, CA) were constructed essentially as in Tian and Lewis 10 . .. Phage titering, library amplification, and plaque lifts were performed according to the manufacturer’s protocols (Stratagene, La Jolla, CA) onto Hybond NX nylon membranes (GE Healthcare).

    Article Title: Investigating hookworm genomes by comparative analysis of two Ancylostoma species
    Article Snippet: .. Approximately 5 μg of mRNA was used to construct directional cDNA libraries in Lambda ZAP II (Stratagene, La Jolla, CA) as previously described [ ]. ..

    Amplification:

    Article Title: The molecular logic for planarian regeneration along the anterior-posterior axis.
    Article Snippet: The planarian Dugesia japonica can regenerate a complete individual from a head, trunk or tail fragment via activation of somatic pluripotent stem cells.. About a century ago, Thomas Hunt Morgan attempted to explain the extraordinary regenerative ability of planarians by positing two opposing morphogenetic gradients of formative ‘‘head stuff’’ and ‘‘tail stuff’’ along the anterior– posterior axis.. However, Morgan’s hypothesis remains open to debate.

    cDNA Library Assay:

    Article Title: The molecular logic for planarian regeneration along the anterior-posterior axis.
    Article Snippet: The planarian Dugesia japonica can regenerate a complete individual from a head, trunk or tail fragment via activation of somatic pluripotent stem cells.. About a century ago, Thomas Hunt Morgan attempted to explain the extraordinary regenerative ability of planarians by positing two opposing morphogenetic gradients of formative ‘‘head stuff’’ and ‘‘tail stuff’’ along the anterior– posterior axis.. However, Morgan’s hypothesis remains open to debate.

    Article Title: Investigating hookworm genomes by comparative analysis of two Ancylostoma species
    Article Snippet: Poly (A)+ RNA was isolated from total RNA using the Oligotex mRNA isolation kit (Qiagen, Chatsworth, CA). .. Approximately 5 μg of mRNA was used to construct a directional cDNA library in Lambda ZAP II (Stratagene, La Jolla, CA) as previously described [ ]. pBluescript phagemid were mass excised prior to sequencing. ..

    Article Title: Piriform Spider Silk Sequences Reveal Unique Repetitive Elements
    Article Snippet: Due to the difficulty in separating aciniform from piriform glands, libraries were constructed from mRNA isolated from piriform-enriched posterior regions. mRNA extraction Fifty to 100mg of individual glands or posterior regions were pooled and homogenized in TRI reagent and total RNA was extracted according to the manufacturer’s protocol (Molecular Research Center, Cincinnati, OH). mRNA was isolated using Dynabeads oligo(dT) 25 (Dynal, Inc, Lake Success, NY) for N. clavipes and A. trifasciata or by the Oligotex kit (Qiagen, Germany) for N. cruentata . .. The mRNA concentration was determined spectrophotometrically. cDNA Library Construction cDNA from N. clavipes and A. trifasciata was synthesized with Superscript II Choice System (Invitrogen, Carlsbad, CA) and Lambda ZAP II libraries (Stratagene, La Jolla, CA) were constructed essentially as in Tian and Lewis 10 . .. Phage titering, library amplification, and plaque lifts were performed according to the manufacturer’s protocols (Stratagene, La Jolla, CA) onto Hybond NX nylon membranes (GE Healthcare).

    Sequencing:

    Article Title: Investigating hookworm genomes by comparative analysis of two Ancylostoma species
    Article Snippet: Poly (A)+ RNA was isolated from total RNA using the Oligotex mRNA isolation kit (Qiagen, Chatsworth, CA). .. Approximately 5 μg of mRNA was used to construct a directional cDNA library in Lambda ZAP II (Stratagene, La Jolla, CA) as previously described [ ]. pBluescript phagemid were mass excised prior to sequencing. ..

    Hybridization:

    Article Title: Experimental Rhodococcus equi and equine infectious anemia virus DNA vaccination in adult and neonatal horses: Effect of IL-12, dose, and route
    Article Snippet: .. For the p40 subunit, a library was made using Lambda Zap II (Stratagene, La Jolla, CA) which was then screened by hybridization with a p40-specific 32 P labeled probe. ..

    Labeling:

    Article Title: Experimental Rhodococcus equi and equine infectious anemia virus DNA vaccination in adult and neonatal horses: Effect of IL-12, dose, and route
    Article Snippet: .. For the p40 subunit, a library was made using Lambda Zap II (Stratagene, La Jolla, CA) which was then screened by hybridization with a p40-specific 32 P labeled probe. ..

    Concentration Assay:

    Article Title: Piriform Spider Silk Sequences Reveal Unique Repetitive Elements
    Article Snippet: Due to the difficulty in separating aciniform from piriform glands, libraries were constructed from mRNA isolated from piriform-enriched posterior regions. mRNA extraction Fifty to 100mg of individual glands or posterior regions were pooled and homogenized in TRI reagent and total RNA was extracted according to the manufacturer’s protocol (Molecular Research Center, Cincinnati, OH). mRNA was isolated using Dynabeads oligo(dT) 25 (Dynal, Inc, Lake Success, NY) for N. clavipes and A. trifasciata or by the Oligotex kit (Qiagen, Germany) for N. cruentata . .. The mRNA concentration was determined spectrophotometrically. cDNA Library Construction cDNA from N. clavipes and A. trifasciata was synthesized with Superscript II Choice System (Invitrogen, Carlsbad, CA) and Lambda ZAP II libraries (Stratagene, La Jolla, CA) were constructed essentially as in Tian and Lewis 10 . .. Phage titering, library amplification, and plaque lifts were performed according to the manufacturer’s protocols (Stratagene, La Jolla, CA) onto Hybond NX nylon membranes (GE Healthcare).

    Synthesized:

    Article Title: Piriform Spider Silk Sequences Reveal Unique Repetitive Elements
    Article Snippet: Due to the difficulty in separating aciniform from piriform glands, libraries were constructed from mRNA isolated from piriform-enriched posterior regions. mRNA extraction Fifty to 100mg of individual glands or posterior regions were pooled and homogenized in TRI reagent and total RNA was extracted according to the manufacturer’s protocol (Molecular Research Center, Cincinnati, OH). mRNA was isolated using Dynabeads oligo(dT) 25 (Dynal, Inc, Lake Success, NY) for N. clavipes and A. trifasciata or by the Oligotex kit (Qiagen, Germany) for N. cruentata . .. The mRNA concentration was determined spectrophotometrically. cDNA Library Construction cDNA from N. clavipes and A. trifasciata was synthesized with Superscript II Choice System (Invitrogen, Carlsbad, CA) and Lambda ZAP II libraries (Stratagene, La Jolla, CA) were constructed essentially as in Tian and Lewis 10 . .. Phage titering, library amplification, and plaque lifts were performed according to the manufacturer’s protocols (Stratagene, La Jolla, CA) onto Hybond NX nylon membranes (GE Healthcare).

    other:

    Article Title: Primary Identification, Biochemical Characterization, and Immunologic Properties of the Allergenic Pollen Cyclophilin Cat r 1
    Article Snippet: The C. roseus cDNA library in Lambda ZAP-II (Stratagene, La Jolla, CA) was kindly provided by Prof. Johan Memelink (University of Leiden, The Netherlands).



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