lambda fix ii vector (Agilent technologies)
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lambda fix ii vector
Lambda Fix Ii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+fix+ii/pmc00538280-103-14-18
Average 90 stars, based on 1 article reviews
Lambda Fix Ii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+fix+ii/pmc00538280-103-14-18
Average 90 stars, based on 1 article reviews
lambda fix ii vector - by Bioz Stars,
2026-09
90/100 stars
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Plasmid Preparation:Article Title: Molecular cloning and expression of the Streptomyces coniferyl alcohol dehydrogenase gene in Escherichia coli. Article Snippet: Coniferyl alcohol dehydrogenase (CADH) is a key enzyme in catabolism of lignin-related aromatic compounds in bacteria.. In Streptomyces sp. NL15-2K, CADH is a tetramer of identical subunits with an individual molecular mass of 39 kDa.. This work describes the cloning and sequencing of the CADH gene from Streptomyces sp. NL15-2K, optimization of a protocol for high-level active CADH expression, and purification of recombinant CADH. Article Title: Intramammary infection caused by Staphylococcus aureus increases IgA antibodies to iron-regulated surface determinant-A, -B, and -H in bovine milk. Article Snippet: The aim of this study was to identify virulence factors that have high immunogenicity.. An in vivo-expressed Staphylococcus aureus antigen was identified by probing bacteriophage expression libraries of S. aureus with antibodies in bovine mastitis milk.. Eighteen clones were isolated, and their proteins were identified as 5 characterised proteins (IsdA, Protein A, IsdB, autolysin, and imidazole glycerol phosphate dehydratase) and 13 hypothetical proteins. Article Title: Characterisation of acyl-ACP desaturases from Macadamia integrifolia Maiden & Betche and Nerium oleander L. Article Snippet: The seed oil in Macadamia integrifolia contains about 30% palmitoleic acid (16:1D9) and Nerium oleander about 12% isoricinoleic acid (D9-hydroxy-18:1D12).. It has been shown that palmitoleic acid can be produced by acyl-acyl carrier protein (ACP) desaturases and it has also been shown that fatty acid hydroxylation can occur via direct substitution of a hydrogen atom.. Therefore it seemed possible that the enzymes responsible for the making of these unusual fatty acids in M. integrifolia and N. oleander were of acyl-ACP desaturase type. Article Title: Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants Article Snippet: The pUC9 MCS was converted to the pBluescript MCS by ligating two oligonucleotides (RPH-212 and RPH-213) to the Eco RI- Hind III cut vector, generating Kpn I and Sac I sites, then inserting a Kpn I- Sac I fragment from pBluescript SKII+ (Stratagene). .. The HortResearch apple EST libraries were constructed in either the Lambda ZAP Express or the Construct:Article Title: Molecular cloning and expression of the Streptomyces coniferyl alcohol dehydrogenase gene in Escherichia coli. Article Snippet: Coniferyl alcohol dehydrogenase (CADH) is a key enzyme in catabolism of lignin-related aromatic compounds in bacteria.. In Streptomyces sp. NL15-2K, CADH is a tetramer of identical subunits with an individual molecular mass of 39 kDa.. This work describes the cloning and sequencing of the CADH gene from Streptomyces sp. NL15-2K, optimization of a protocol for high-level active CADH expression, and purification of recombinant CADH. Article Title: Characterisation of acyl-ACP desaturases from Macadamia integrifolia Maiden & Betche and Nerium oleander L. Article Snippet: The seed oil in Macadamia integrifolia contains about 30% palmitoleic acid (16:1D9) and Nerium oleander about 12% isoricinoleic acid (D9-hydroxy-18:1D12).. It has been shown that palmitoleic acid can be produced by acyl-acyl carrier protein (ACP) desaturases and it has also been shown that fatty acid hydroxylation can occur via direct substitution of a hydrogen atom.. Therefore it seemed possible that the enzymes responsible for the making of these unusual fatty acids in M. integrifolia and N. oleander were of acyl-ACP desaturase type. Article Title: Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants Article Snippet: The pUC9 MCS was converted to the pBluescript MCS by ligating two oligonucleotides (RPH-212 and RPH-213) to the Eco RI- Hind III cut vector, generating Kpn I and Sac I sites, then inserting a Kpn I- Sac I fragment from pBluescript SKII+ (Stratagene). .. The HortResearch apple EST libraries were constructed in either the Lambda ZAP Express or the Article Title: Intramammary infection caused by Staphylococcus aureus increases IgA antibodies to iron-regulated surface determinant-A, -B, and -H in bovine milk. Article Snippet: The aim of this study was to identify virulence factors that have high immunogenicity.. An in vivo-expressed Staphylococcus aureus antigen was identified by probing bacteriophage expression libraries of S. aureus with antibodies in bovine mastitis milk.. Eighteen clones were isolated, and their proteins were identified as 5 characterised proteins (IsdA, Protein A, IsdB, autolysin, and imidazole glycerol phosphate dehydratase) and 13 hypothetical proteins. Clone Assay:Article Title: Microsatellite markers isolated in olive ( Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Article Snippet: We have isolated and sequenced 52 microsatellites or simple sequence repeats (SSRs) from nearly 60 positive clones obtained from two ‘Frantoio’ olive genomic libraries enriched in (AC/GT) and (AG/CT) repeats, respectively.. The repeat-containing fragments obtained from genomic DNA restricted with Tsp509I were separated using a biotinylated probe bound to streptavidin-coated paramagnetic beads.. Fragments were then cloned into lambda ZAPII vector and sequenced. Article Title: Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants Article Snippet: The pUC9 MCS was converted to the pBluescript MCS by ligating two oligonucleotides (RPH-212 and RPH-213) to the Eco RI- Hind III cut vector, generating Kpn I and Sac I sites, then inserting a Kpn I- Sac I fragment from pBluescript SKII+ (Stratagene). .. The HortResearch apple EST libraries were constructed in either the Lambda ZAP Express or the Article Title: Characterization of the chicken inward rectifier K + channel IRK1/Kir2.1 gene Article Snippet: .. Chick IRK1 genomic clones were isolated by screening a chick genomic library in the other:Article Title: Xylanolytic Extremozymes Retrieved From Environmental Metagenomes: Characteristics, Genetic Engineering, and Applications Article Snippet: 4. , Cow rumen metagenome , RUM630-BX , Lambda ZAP II Vector and Isolation:Article Title: Characterisation of acyl-ACP desaturases from Macadamia integrifolia Maiden & Betche and Nerium oleander L. Article Snippet: The seed oil in Macadamia integrifolia contains about 30% palmitoleic acid (16:1D9) and Nerium oleander about 12% isoricinoleic acid (D9-hydroxy-18:1D12).. It has been shown that palmitoleic acid can be produced by acyl-acyl carrier protein (ACP) desaturases and it has also been shown that fatty acid hydroxylation can occur via direct substitution of a hydrogen atom.. Therefore it seemed possible that the enzymes responsible for the making of these unusual fatty acids in M. integrifolia and N. oleander were of acyl-ACP desaturase type. Article Title: Characterization of the chicken inward rectifier K + channel IRK1/Kir2.1 gene Article Snippet: .. Chick IRK1 genomic clones were isolated by screening a chick genomic library in the Expressing:Article Title: Intramammary infection caused by Staphylococcus aureus increases IgA antibodies to iron-regulated surface determinant-A, -B, and -H in bovine milk. Article Snippet: The aim of this study was to identify virulence factors that have high immunogenicity.. An in vivo-expressed Staphylococcus aureus antigen was identified by probing bacteriophage expression libraries of S. aureus with antibodies in bovine mastitis milk.. Eighteen clones were isolated, and their proteins were identified as 5 characterised proteins (IsdA, Protein A, IsdB, autolysin, and imidazole glycerol phosphate dehydratase) and 13 hypothetical proteins. |