Review



sureprint g3 human gene expression v3 8x60k format cdna microarray  (Agilent technologies)


Bioz Verified Symbol Agilent technologies is a verified supplier
Bioz Manufacturer Symbol Agilent technologies manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Agilent technologies sureprint g3 human gene expression v3 8x60k format cdna microarray
    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA <t> microarray </t> dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Sureprint G3 Human Gene Expression V3 8x60k Format Cdna Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/pmc11054952-66-17-16
    Average 90 stars, based on 1 article reviews
    sureprint g3 human gene expression v3 8x60k format cdna microarray - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes"

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes

    Journal: Non-Coding RNA

    doi: 10.3390/ncrna10020025

    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA  microarray  dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Figure Legend Snippet: Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA microarray dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.

    Techniques Used: Microarray, Software

    Top ten downregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA  microarray  dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Figure Legend Snippet: Top ten downregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA microarray dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.

    Techniques Used: Microarray, Software

    Difference in lncRNA and mRNA fold-changes between the cDNA microarray and in-house RT-qPCR experiments. Data shown are from three independent experiments (in triplicates) with calculated SD values to represent error bars and their statistical analyses were conducted using one-way ANOVA.
    Figure Legend Snippet: Difference in lncRNA and mRNA fold-changes between the cDNA microarray and in-house RT-qPCR experiments. Data shown are from three independent experiments (in triplicates) with calculated SD values to represent error bars and their statistical analyses were conducted using one-way ANOVA.

    Techniques Used: Microarray, Quantitative RT-PCR

    ( A ) LncRNA-mRNA interaction network was constructed based on the ten selected candidate lncRNAs from the cDNA microarray dataset. The resulting mRNA interactions were predicted using the “rtool” database with −20 kcal as the minimum energy threshold and were filtered to show only mRNAs that were also dysregulated in the dataset. Data were visualized using Cytoscape v3.8.2. (Pink diamond = candidate lncRNA, blue circle = predicted putative mRNA targets). ( B ) A total of nine mRNAs were identified as both responsible in the “regulated exocytosis” hit as well as highly likely to be regulated by the candidate lncRNAs listed in ( A ). Data were visualized using Cytoscape v3.8.2. (pink diamond = candidate regulator lncRNA, blue circle = mRNA predicted involved in the biological process).
    Figure Legend Snippet: ( A ) LncRNA-mRNA interaction network was constructed based on the ten selected candidate lncRNAs from the cDNA microarray dataset. The resulting mRNA interactions were predicted using the “rtool” database with −20 kcal as the minimum energy threshold and were filtered to show only mRNAs that were also dysregulated in the dataset. Data were visualized using Cytoscape v3.8.2. (Pink diamond = candidate lncRNA, blue circle = predicted putative mRNA targets). ( B ) A total of nine mRNAs were identified as both responsible in the “regulated exocytosis” hit as well as highly likely to be regulated by the candidate lncRNAs listed in ( A ). Data were visualized using Cytoscape v3.8.2. (pink diamond = candidate regulator lncRNA, blue circle = mRNA predicted involved in the biological process).

    Techniques Used: Construct, Microarray

    Related Articles

    Microarray:

    Article Title: Engineering Toxoplasma gondii secretion systems for intracellular delivery of multiple large therapeutic proteins to neurons
    Article Snippet: All hiPSC lines employed were routinely checked for mycoplasma contamination and the correct cell line identity was confirmed through short tandem repeats profiling with the kit GenePrintR 10 system (Promega). .. Cell lines were profiled with Array-CGH to verify the absence of chromosomal rearrangements, using the Agilent kit SurePrint G3 Human CGH Microarray 8x60K. ..

    Article Title: Comprehensive multi-omics analysis of breast cancer reveals distinct long-term prognostic subtypes
    Article Snippet: .. SurePrint G3 Human GE 8x60K (Agilent Technologies) was used to measure mRNA expression as per the manufactory’s protocol (one-color microarray-based gene expression analysis, low input Quick Amp Labeling, v.6.5, May 2010) and 100 ng RNA was used as input for labeling. ..

    Article Title: Prognostic Value of Tumor Tissue Up-regulated microRNAs in Clear Cell Renal Cell Carcinoma (ccRCC)
    Article Snippet: .. MicroRNA expression profiling was performed employing the SurePrint G3 Human miRNA Release 21 microarray kit (8x60K, Agilent Technologies, Santa Clara, CA, USA). .. RNA extracted from the samples was prepared following the protocol of the miRNA Complete Labelling and Hybridization Kit (Agilent Technologies), without the use of an optional spike-in control or subsequent purification of labelled RNA.

    Article Title: DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells
    Article Snippet: For microarray analysis, RNA quality was assessed using a Bioanalyzer (Agilent) and then quantified using a Biospecnano instrument (Shimadzu, Kyoto, Japan). .. Gene expression analysis was performed using an Agilent® SurePrint G3 Human GE 8x60K Microarray (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Single Color Labeling Kit (Low Input Quick Amp Labeling Kit 034949) adapted for small amounts of total RNA (100 ng total RNA per reaction). ..

    Article Title: Engineering Toxoplasma gondii secretion systems for intracellular delivery of multiple large therapeutic proteins to neurons.
    Article Snippet: All hiPSC lines employed were routinely checked for mycoplasma contamination and the correct cell line identity was confirmed through short tandem repeats profiling with the kit GenePrintR 10 system (Promega). .. Cell lines were profiled with Array-CGH to verify the absence of chromosomal rearrangements, using the Agilent kit SurePrint G3 Human CGH Microarray 8x60K. ..

    Article Title: Knockout of the orphan membrane transporter Slc22a23 leads to a lean and hyperactive phenotype with a small hippocampal volume.
    Article Snippet: Embryonic forebrain microdissections were isolated from the 10-μm thick sections using a laser microdissection system (Molecular Machines and Industries GmbH). mRNA samples were extracted from the microdissections and converted to cDNA. .. After Cy3-labeling, the cDNA samples were hybridized to the SurePrint G3 Rat GE3 8x60K microarray (Agilent Technologies, Santa Clara, CA, US). ..

    Expressing:

    Article Title: Comprehensive multi-omics analysis of breast cancer reveals distinct long-term prognostic subtypes
    Article Snippet: .. SurePrint G3 Human GE 8x60K (Agilent Technologies) was used to measure mRNA expression as per the manufactory’s protocol (one-color microarray-based gene expression analysis, low input Quick Amp Labeling, v.6.5, May 2010) and 100 ng RNA was used as input for labeling. ..

    Article Title: Prognostic Value of Tumor Tissue Up-regulated microRNAs in Clear Cell Renal Cell Carcinoma (ccRCC)
    Article Snippet: .. MicroRNA expression profiling was performed employing the SurePrint G3 Human miRNA Release 21 microarray kit (8x60K, Agilent Technologies, Santa Clara, CA, USA). .. RNA extracted from the samples was prepared following the protocol of the miRNA Complete Labelling and Hybridization Kit (Agilent Technologies), without the use of an optional spike-in control or subsequent purification of labelled RNA.

    Gene Expression:

    Article Title: Comprehensive multi-omics analysis of breast cancer reveals distinct long-term prognostic subtypes
    Article Snippet: .. SurePrint G3 Human GE 8x60K (Agilent Technologies) was used to measure mRNA expression as per the manufactory’s protocol (one-color microarray-based gene expression analysis, low input Quick Amp Labeling, v.6.5, May 2010) and 100 ng RNA was used as input for labeling. ..

    Article Title: DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells
    Article Snippet: For microarray analysis, RNA quality was assessed using a Bioanalyzer (Agilent) and then quantified using a Biospecnano instrument (Shimadzu, Kyoto, Japan). .. Gene expression analysis was performed using an Agilent® SurePrint G3 Human GE 8x60K Microarray (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Single Color Labeling Kit (Low Input Quick Amp Labeling Kit 034949) adapted for small amounts of total RNA (100 ng total RNA per reaction). ..

    Labeling:

    Article Title: Comprehensive multi-omics analysis of breast cancer reveals distinct long-term prognostic subtypes
    Article Snippet: .. SurePrint G3 Human GE 8x60K (Agilent Technologies) was used to measure mRNA expression as per the manufactory’s protocol (one-color microarray-based gene expression analysis, low input Quick Amp Labeling, v.6.5, May 2010) and 100 ng RNA was used as input for labeling. ..

    Article Title: DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells
    Article Snippet: For microarray analysis, RNA quality was assessed using a Bioanalyzer (Agilent) and then quantified using a Biospecnano instrument (Shimadzu, Kyoto, Japan). .. Gene expression analysis was performed using an Agilent® SurePrint G3 Human GE 8x60K Microarray (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Single Color Labeling Kit (Low Input Quick Amp Labeling Kit 034949) adapted for small amounts of total RNA (100 ng total RNA per reaction). ..

    Article Title: A novel selective NLRP3 inhibitor shows disease-modifying potential in animal models of Parkinson's disease.
    Article Snippet: Pathological activation of the Nod-like receptor family pyrin domain containing protein 3 (NLRP3) inflammasome signaling underlies many autoimmune and neuroinflammatory conditions.. Here we report that, a rationally designed, novel, orally active, selective NLRP3



    Similar Products

    90
    Agilent technologies sureprint g3 human gene expression v3 8x60k format cdna microarray
    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA <t> microarray </t> dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Sureprint G3 Human Gene Expression V3 8x60k Format Cdna Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/pmc11054952-66-17-16
    Average 90 stars, based on 1 article reviews
    sureprint g3 human gene expression v3 8x60k format cdna microarray - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher cdna microarray genechip human genome hg-u133a
    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA <t> microarray </t> dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Cdna Microarray Genechip Human Genome Hg U133a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/seebluetm+plus2+pre+stained+protein+standard/pmc12070984-99-0-7
    Average 90 stars, based on 1 article reviews
    cdna microarray genechip human genome hg-u133a - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    93
    OriGene hnscc cdna microarray
    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA <t> microarray </t> dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Hnscc Cdna Microarray, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/C3AR1+(NM_004054)+Human+Untagged+Clone/pm40482908-135-7-10
    Average 93 stars, based on 1 article reviews
    hnscc cdna microarray - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    90
    Thermo Fisher cdna microarray genechip human genome hgu133a
    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA <t> microarray </t> dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Cdna Microarray Genechip Human Genome Hgu133a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/seebluetm+plus2+pre+stained+protein+standard/pm40361356-70-0-7
    Average 90 stars, based on 1 article reviews
    cdna microarray genechip human genome hgu133a - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Shenzhen ChipScreen Biosciences Ltd 8k human cdna microarrays
    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA <t> microarray </t> dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    8k Human Cdna Microarrays, supplied by Shenzhen ChipScreen Biosciences Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/8k+human+cdna+microarrays/pm39286844-42-16-21
    Average 90 stars, based on 1 article reviews
    8k human cdna microarrays - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Genomictree Inc cdna microarray with 17,448 sequence-verified human cdna clones
    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA <t> microarray </t> dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Cdna Microarray With 17,448 Sequence Verified Human Cdna Clones, supplied by Genomictree Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/cdna+microarray++containing+a+set+of+17+448+sequence+verified+human+cdna+clones/pm38670589-65-5-12
    Average 90 stars, based on 1 article reviews
    cdna microarray with 17,448 sequence-verified human cdna clones - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Incyte corporation human cdna microarrays
    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA <t> microarray </t> dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
    Human Cdna Microarrays, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/human+cdna+microarrays/us11618922-228-2-29
    Average 90 stars, based on 1 article reviews
    human cdna microarrays - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Unigene human unigene set rzpd 1 clone set for cdna microarrays
    Comparisons of real-time quantitative reverse transcription PCR, <t> microarrays, </t> and RNA-sequencing and their applications in hepatocellular carcinoma recurrence
    Human Unigene Set Rzpd 1 Clone Set For Cdna Microarrays, supplied by Unigene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/cdna+arrays+human+unigene+set+rzpd+1+clone+set/pmc09932421-102-15-8
    Average 90 stars, based on 1 article reviews
    human unigene set rzpd 1 clone set for cdna microarrays - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies whole human genome oligo microarray 44 k cdna array
    Comparisons of real-time quantitative reverse transcription PCR, <t> microarrays, </t> and RNA-sequencing and their applications in hepatocellular carcinoma recurrence
    Whole Human Genome Oligo Microarray 44 K Cdna Array, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cdna+microarray/pmc01779497-69-70-72
    Average 90 stars, based on 1 article reviews
    whole human genome oligo microarray 44 k cdna array - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA  microarray  dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.

    Journal: Non-Coding RNA

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes

    doi: 10.3390/ncrna10020025

    Figure Lengend Snippet: Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA microarray dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.

    Article Snippet: In this study, purified total RNAs of both the SW480/DR and SW480/DS cells were analyzed using Agilent SurePrint G3 Human Gene Expression v3 8x60k format cDNA microarray and processed in the Agilent GeneSpring GX v14.9.1 software.

    Techniques: Microarray, Software

    Top ten downregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA  microarray  dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.

    Journal: Non-Coding RNA

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes

    doi: 10.3390/ncrna10020025

    Figure Lengend Snippet: Top ten downregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA microarray dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.

    Article Snippet: In this study, purified total RNAs of both the SW480/DR and SW480/DS cells were analyzed using Agilent SurePrint G3 Human Gene Expression v3 8x60k format cDNA microarray and processed in the Agilent GeneSpring GX v14.9.1 software.

    Techniques: Microarray, Software

    Difference in lncRNA and mRNA fold-changes between the cDNA microarray and in-house RT-qPCR experiments. Data shown are from three independent experiments (in triplicates) with calculated SD values to represent error bars and their statistical analyses were conducted using one-way ANOVA.

    Journal: Non-Coding RNA

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes

    doi: 10.3390/ncrna10020025

    Figure Lengend Snippet: Difference in lncRNA and mRNA fold-changes between the cDNA microarray and in-house RT-qPCR experiments. Data shown are from three independent experiments (in triplicates) with calculated SD values to represent error bars and their statistical analyses were conducted using one-way ANOVA.

    Article Snippet: In this study, purified total RNAs of both the SW480/DR and SW480/DS cells were analyzed using Agilent SurePrint G3 Human Gene Expression v3 8x60k format cDNA microarray and processed in the Agilent GeneSpring GX v14.9.1 software.

    Techniques: Microarray, Quantitative RT-PCR

    ( A ) LncRNA-mRNA interaction network was constructed based on the ten selected candidate lncRNAs from the cDNA microarray dataset. The resulting mRNA interactions were predicted using the “rtool” database with −20 kcal as the minimum energy threshold and were filtered to show only mRNAs that were also dysregulated in the dataset. Data were visualized using Cytoscape v3.8.2. (Pink diamond = candidate lncRNA, blue circle = predicted putative mRNA targets). ( B ) A total of nine mRNAs were identified as both responsible in the “regulated exocytosis” hit as well as highly likely to be regulated by the candidate lncRNAs listed in ( A ). Data were visualized using Cytoscape v3.8.2. (pink diamond = candidate regulator lncRNA, blue circle = mRNA predicted involved in the biological process).

    Journal: Non-Coding RNA

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes

    doi: 10.3390/ncrna10020025

    Figure Lengend Snippet: ( A ) LncRNA-mRNA interaction network was constructed based on the ten selected candidate lncRNAs from the cDNA microarray dataset. The resulting mRNA interactions were predicted using the “rtool” database with −20 kcal as the minimum energy threshold and were filtered to show only mRNAs that were also dysregulated in the dataset. Data were visualized using Cytoscape v3.8.2. (Pink diamond = candidate lncRNA, blue circle = predicted putative mRNA targets). ( B ) A total of nine mRNAs were identified as both responsible in the “regulated exocytosis” hit as well as highly likely to be regulated by the candidate lncRNAs listed in ( A ). Data were visualized using Cytoscape v3.8.2. (pink diamond = candidate regulator lncRNA, blue circle = mRNA predicted involved in the biological process).

    Article Snippet: In this study, purified total RNAs of both the SW480/DR and SW480/DS cells were analyzed using Agilent SurePrint G3 Human Gene Expression v3 8x60k format cDNA microarray and processed in the Agilent GeneSpring GX v14.9.1 software.

    Techniques: Construct, Microarray

    Comparisons of real-time quantitative reverse transcription PCR,  microarrays,  and RNA-sequencing and their applications in hepatocellular carcinoma recurrence

    Journal: World Journal of Gastroenterology

    Article Title: Transcriptome analysis creates a new era of precision medicine for managing recurrent hepatocellular carcinoma

    doi: 10.3748/wjg.v29.i5.780

    Figure Lengend Snippet: Comparisons of real-time quantitative reverse transcription PCR, microarrays, and RNA-sequencing and their applications in hepatocellular carcinoma recurrence

    Article Snippet: The surfacing of commercial platforms, such as Human UniGene Set RZPD 1 clone set for cDNA microarrays and Affymetrix Human Genome U95Av2 array for oligonucleotide arrays, have made the technology for both types of arrays widely accessible[ ].

    Techniques: Isolation, cDNA Library Assay, Amplification, Labeling, Fluorescence, Hybridization, Sequencing, High Throughput Screening Assay