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hplc method  (Agilent technologies)


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    Structured Review

    Agilent technologies hplc method
    <t>HPLC</t> chromatogram of the aqueous extract of Entandrophragma cylindricum showing absorbance (mAU) as a function of retention time (see ).
    Hplc Method, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 65038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hplc+method/Bio+LC/pmc13694897-187-0-2
    Average 95 stars, based on 65038 article reviews
    hplc method - by Bioz Stars, 2026-11
    95/100 stars

    Images

    1) Product Images from "Neuroprotective effects of Entandrophragma cylindricum (Meliaceae) extract against “Odontol’’-induced cognitive and depressive disorders: An integrated HPLC, molecular docking and experimental validation"

    Article Title: Neuroprotective effects of Entandrophragma cylindricum (Meliaceae) extract against “Odontol’’-induced cognitive and depressive disorders: An integrated HPLC, molecular docking and experimental validation

    Journal: IBRO Neuroscience Reports

    doi: 10.1016/j.ibneur.2026.09.019

    HPLC chromatogram of the aqueous extract of Entandrophragma cylindricum showing absorbance (mAU) as a function of retention time (see ).
    Figure Legend Snippet: HPLC chromatogram of the aqueous extract of Entandrophragma cylindricum showing absorbance (mAU) as a function of retention time (see ).

    Techniques Used:

    Related Articles

    Solvent:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    High Performance Liquid Chromatography:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Membrane:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Gel Permeation Chromatography:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Chromatography:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Molecular Weight:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Synthesized:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    GPC Assay:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Targeted Proteomics:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Mass Spectrometry:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Injection:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Liquid Chromatography:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Concentration Assay:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Spectrophotometry:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Pore Size:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Lyophilization:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Incubation:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl

    Centrifugation:

    Article Title: Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
    Article Snippet: 000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane filter. The filtrate was collected for HPLC analysis. HPLC analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent ZORBAX Eclipse Plus C18 column (5 μm, 4.6 × 250 mm). The mobile phase consisted of solvent A (ultrapure water containing 0.1% trifl



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    Image Search Results


    A. Development of an optimized bioinert LC-MS/MS workflow for modified nucleoside analysis. Comparison of the reference (left) and optimized (right) workflows, highlighting the replacement of the conventional LC column and mobile phase with a Synergy Hydro-RP bioinert column and optimized mobile-phase composition. Common analytical steps include autosampling, chromatographic separation, ionization, and MS/MS detection. B. Comparison of nucleoside retention times obtained using the reference method (5 mM ammonium acetate, Synergi Fusion-RP column) and the new method (0.5% acetic acid, Synergi Hydro-RP column). The dashed line represents the line of identity (y = x). C. Comparison of fold-change values obtained using the new method relative to the reference method for canonical nucleosides. Fold-change values were calculated relative to the reference method, which was set to 1. Data are presented as mean ± SD. D. Comparison of fold-change values obtained using the new method relative to the reference method for uridine and selected uridine-derived modifications. Fold-change values were calculated relative to the reference method, which was set to 1. Bars represent mean values, and error bars indicate SD. E. Effect of transferring the method to a bioinert LC system on nucleoside detection sensitivity. Data are presented as the ratio of peak areas obtained with the bioinert LC system (LC XSi) to those obtained with the conventional LC-40 system.

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    Figure Lengend Snippet: A. Development of an optimized bioinert LC-MS/MS workflow for modified nucleoside analysis. Comparison of the reference (left) and optimized (right) workflows, highlighting the replacement of the conventional LC column and mobile phase with a Synergy Hydro-RP bioinert column and optimized mobile-phase composition. Common analytical steps include autosampling, chromatographic separation, ionization, and MS/MS detection. B. Comparison of nucleoside retention times obtained using the reference method (5 mM ammonium acetate, Synergi Fusion-RP column) and the new method (0.5% acetic acid, Synergi Hydro-RP column). The dashed line represents the line of identity (y = x). C. Comparison of fold-change values obtained using the new method relative to the reference method for canonical nucleosides. Fold-change values were calculated relative to the reference method, which was set to 1. Data are presented as mean ± SD. D. Comparison of fold-change values obtained using the new method relative to the reference method for uridine and selected uridine-derived modifications. Fold-change values were calculated relative to the reference method, which was set to 1. Bars represent mean values, and error bars indicate SD. E. Effect of transferring the method to a bioinert LC system on nucleoside detection sensitivity. Data are presented as the ratio of peak areas obtained with the bioinert LC system (LC XSi) to those obtained with the conventional LC-40 system.

    Article Snippet: The reference method was performed on a Nexera LC-40 system equipped with a Synergi Fusion-RP column (250×2mm, 4μm, 80A, 00G-4424-B0, Phenomenex).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Modification, Analysis, Comparison, Tandem Mass Spectroscopy, Derivative Assay, Transferring, Sensitivity

    HPLC chromatogram of the aqueous extract of Entandrophragma cylindricum showing absorbance (mAU) as a function of retention time (see ).

    Journal: IBRO Neuroscience Reports

    Article Title: Neuroprotective effects of Entandrophragma cylindricum (Meliaceae) extract against “Odontol’’-induced cognitive and depressive disorders: An integrated HPLC, molecular docking and experimental validation

    doi: 10.1016/j.ibneur.2026.09.019

    Figure Lengend Snippet: HPLC chromatogram of the aqueous extract of Entandrophragma cylindricum showing absorbance (mAU) as a function of retention time (see ).

    Article Snippet: HPLC method (Agilent Chemstation 1260 infinity.

    Techniques: