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high stringency wash buffer  (Active Motif)


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    Structured Review

    Active Motif high stringency wash buffer
    High Stringency Wash Buffer, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+wash+buffer/high+stringency+buffer+option/pm34013328-81-7-6
    Average 90 stars, based on 1 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Cohesin stabilization at promoters and enhancers by common transcription factors and chromatin regulators.
    Article Snippet: Cold High-stringency co-IP Buffer (per Active Motif Nuclear Complex Co-IP Kit (54001) instructions, 37510) was added to each sample to achieve equal volumes, then antibodies conjugated to beads were added and samples were incubated overnight, rotating at 4 °C.

    Co-Immunoprecipitation Assay:

    Article Title: AUTS2 Regulates RNA Metabolism and Dentate Gyrus Development in Mice.
    Article Snippet: Human AUTS2 mutations are linked to a syndrome of intellectual disability, autistic features, epilepsy, and other neurological and somatic disorders.. Although it is known that this unique gene is highly expressed in developing cerebral cortex, the molecular and developmental functions of AUTS2 protein remain unclear.. Using proteomics methods to identify AUTS2 binding partners in neonatal mouse cerebral cortex, we found that AUTS2 associates with multiple proteins that regulate RNA transcription, splicing, localization, and stability.

    Immunoprecipitation:

    Article Title: AUTS2 Regulates RNA Metabolism and Dentate Gyrus Development in Mice.
    Article Snippet: Human AUTS2 mutations are linked to a syndrome of intellectual disability, autistic features, epilepsy, and other neurological and somatic disorders.. Although it is known that this unique gene is highly expressed in developing cerebral cortex, the molecular and developmental functions of AUTS2 protein remain unclear.. Using proteomics methods to identify AUTS2 binding partners in neonatal mouse cerebral cortex, we found that AUTS2 associates with multiple proteins that regulate RNA transcription, splicing, localization, and stability.

    Bradford Assay:

    Article Title: Quantitative profiling of adaptation to cyclin E overproduction
    Article Snippet: Lysates were precleared for 45 min with magnetic beads (Dynabeads protein G, Cat. no. 10003D; Invitrogen) and nuclear digestion buffer (10 mM Hepes, pH 7.9, 10 mM KCl, 1.5 mM MgCl 2 , 340 mM sucrose, and 0.1 mM glycerol) + 1 mM ATP + protease/phosphatase inhibitors (used above). .. After, quantification via Qubit or Bradford assay , precleared lysates were adjusted to 1× high stringency IP buffer (Cat. no. 37510; Active Motif) with protease/phosphatase inhibitors, 0.01 mM ATP, and a non-ionic non-denaturing detergent (Cat. no. 37517; Active Motif). ..

    Mass Spectrometry:

    Article Title: Loss of Nucleolar Histone Chaperone NPM1 Triggers Rearrangement of Heterochromatin and Synergizes with a Deficiency in DNA Methyltransferase DNMT3A to Drive Ribosomal DNA Transcription
    Article Snippet: .. In the case of large-scale co-IP followed by mass spectrometry, the cells were harvested and prepared according to the nuclear complex co-IP kit instructions using the high stringency buffer option (Active Motif). .. Immunoprecipitates were separated by SDS-PAGE, and gels were stained with colloidal CBB kit according to the manufacturer's instructions (Invitrogen).

    Article Title: Loss of Nucleolar Histone Chaperone NPM1 Triggers Rearrangement of Heterochromatin and Synergizes with a Deficiency in DNA Methyltransferase DNMT3A to Drive Ribosomal DNA Transcription
    Article Snippet: .. In the case of large-scale co-IP followed by mass spectrometry, the cells were harvested and prepared according to the nuclear complex co-IP kit instructions using the high stringency buffer option (Active Motif). .. Immunoprecipitates were separated by SDS-PAGE, and gels were stained with colloidal CBB kit according to the manufacturer ́s instructions (Invitrogen).



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    Key Resources Table

    Journal: Cell

    Article Title: Promoter of lncRNA gene PVT1 is a tumor suppressor DNA boundary element

    doi: 10.1016/j.cell.2018.03.068

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: NorthernMaxTM High Stringency Wash Buffer , Thermo Fisher , Cat#AM8674.

    Techniques: Virus, Recombinant, Ligation, Transfection, Immunoprecipitation, Bradford Assay, Reverse Transcription, Membrane, Blocking Assay, SYBR Green Assay, Sample Prep, Luciferase, Reporter Assay, Clone Assay, Northern Blot, Plasmid Preparation, Cloning, Software