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northernmax high stringency wash buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher northernmax high stringency wash buffer
    Northernmax High Stringency Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+wash+buffer/pmc09638912-70-15-20
    Average 90 stars, based on 1 article reviews
    northernmax high stringency wash buffer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: OASIS/CREB3L1 Induces Expression of Genes Involved in Extracellular Matrix Production But Not Classical Endoplasmic Reticulum Stress Response Genes in Pancreatic ?-Cells
    Article Snippet: The detection probe (25 p m ) was added to hybridization buffer (same buffer used in prehybridization) and incubated at 42 C for 16–18 h. After hybridization, the blot was subjected to washings (23 C/5min, 42 C/15 min (times 2) with NorthernMax low-stringency wash buffer (Ambion).

    Article Title: Generation of an 870 kb deletion encompassing the Skt/Etl4 locus by combination of inter- and intra-chromosomal recombination
    Article Snippet: Unspecific probe was removed by washing with NorthernMax® Low Stringency Wash buffer (Ambion) twice for five minutes and once with NorthernMax® High Stringency Wash buffer (Ambion) for 15 min at 42 °C.

    Article Title: Evaluating Posttranscriptional Regulation of Cytokine Genes
    Article Snippet: NorthernMax® Low Stringency Wash Buffer (Ambion).

    Article Title: Evaluating Posttranscriptional Regulation of Cytokine Genes
    Article Snippet: NorthernMax® High Stringency Wash Buffer (Ambion).

    Membrane:

    Article Title: Expansion of the genetic code through reassignment of redundant sense codons using fully modified tRNA
    Article Snippet: .. The following morning, the membrane was washed with 2 × 10 ml of pre-heated (42°C) NorthernMax High Stringency Wash Buffer (Invitrogen), each wash rocking at 42°C for 15 min. Staining buffer was prepared in the dark by mixing 100 μl of 10% sodium dodecylsulfate (SDS), 50 μl of 20% Tween-20 and 10 μl of IRDye 800 CW Streptavidin (Licor, 926-32230) and diluting to 10 ml in 1× phosphate-buffered saline (PBS) buffer. ..

    Staining:

    Article Title: Expansion of the genetic code through reassignment of redundant sense codons using fully modified tRNA
    Article Snippet: .. The following morning, the membrane was washed with 2 × 10 ml of pre-heated (42°C) NorthernMax High Stringency Wash Buffer (Invitrogen), each wash rocking at 42°C for 15 min. Staining buffer was prepared in the dark by mixing 100 μl of 10% sodium dodecylsulfate (SDS), 50 μl of 20% Tween-20 and 10 μl of IRDye 800 CW Streptavidin (Licor, 926-32230) and diluting to 10 ml in 1× phosphate-buffered saline (PBS) buffer. ..

    Saline:

    Article Title: Expansion of the genetic code through reassignment of redundant sense codons using fully modified tRNA
    Article Snippet: .. The following morning, the membrane was washed with 2 × 10 ml of pre-heated (42°C) NorthernMax High Stringency Wash Buffer (Invitrogen), each wash rocking at 42°C for 15 min. Staining buffer was prepared in the dark by mixing 100 μl of 10% sodium dodecylsulfate (SDS), 50 μl of 20% Tween-20 and 10 μl of IRDye 800 CW Streptavidin (Licor, 926-32230) and diluting to 10 ml in 1× phosphate-buffered saline (PBS) buffer. ..

    Hybridization:

    Article Title: An optimized isolation and labeling platform for accurate microRNA expression profiling
    Article Snippet: For Northern analyses, RNA samples were resolved on denaturing 15% polyacrylamide gels and electroblotted on BrightStar-Plus Nylon membranes (Ambion). .. Membranes were blocked in UltraHyb-oligo Hybridization Buffer (Ambion) for at least 1 h at 65°C, hybridized overnight at 42°C with the appropriate probe, and washed three times with NorthernMax Low Stringency Wash Buffer (Ambion) for 5 min at room temperature followed by 15 min at 42°C. .. Results were quantified on a PhosphorImager Storm 860 (Amersham Bioscience).



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    Key Resources Table

    Journal: Cell

    Article Title: Promoter of lncRNA gene PVT1 is a tumor suppressor DNA boundary element

    doi: 10.1016/j.cell.2018.03.068

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: NorthernMaxTM High Stringency Wash Buffer , Thermo Fisher , Cat#AM8674.

    Techniques: Virus, Recombinant, Ligation, Transfection, Immunoprecipitation, Bradford Assay, Reverse Transcription, Membrane, Blocking Assay, SYBR Green Assay, Sample Prep, Luciferase, Reporter Assay, Clone Assay, Northern Blot, Plasmid Preparation, Cloning, Software