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Lumonics Inc laser scanner scanarray 4000
Laser Scanner Scanarray 4000, supplied by Lumonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+microarray+laser+scanner/scanarray+4000/pm17207603-195-10-18
Average 90 stars, based on 1 article reviews
laser scanner scanarray 4000 - by Bioz Stars, 2026-10
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Related Articles

other:

Article Title: Single-dose combination nanovaccine induces both rapid and long-lived protection against pneumonic plague
Article Snippet: The scanned images of the microarray slides were analyzed using SoftWorRx Tracker v2.8 software (Applied Precision, Inc., Issaquah, WA) to detect and quantify the fluorescence of each spot.

Article Title: Analysis of swine antigen-specific antibody responses to Mycoplasma hyopneumoniae infection determined by protein microarray.
Article Snippet: Accepted Manuscript Title: Analysis of swine antigen-specific antibody responses to Mycoplasma hyopneumoniae infection determined by protein microarray Authors: Andrew C. Petersen, Jeannett M. Clampitt, F. Chris Minion PII: S0378-1135(18)31028-9 DOI: https://doi.org/10.1016/j.vetmic.2019.02.010 Reference: VETMIC 8214 To appear in: VETMIC Received date: 31 August 2018 Revised date: 9 January 2019 Accepted date: 5 February 2019 Please cite this article as: Petersen AC, Clampitt JM, Minion FC, Analysis of swine antigen-specific antibody responses to Mycoplasma hyopneumoniae infection determined by protein microarray, Veterinary Microbiology (2019), https://doi.org/10.1016/j.vetmic.2019.02.010 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Preliminary evaluations on development of new materials for hip joint femoral head
Article Snippet: GTPase Protocols M E T H O D S I N M O L E C U L A R B I O L O G Y TM John M. Walker, Series Editor 216.. PCR Detection of Microbial Pathogens: Methods and Pro- tocols, edited by Konrad Sachse and Joachim Frey, 2003 215.. Cytokines and Colony Stimulating Factors: Methods and Protocols, edited by Dieter Körholz and Wieland Kiess, 2003 214.

Hybridization:

Article Title: Method of designing addressable array suitable for detection of nucleic acid sequence differences using ligase detection reaction
Article Snippet: .. Following hybridization, the arrays were washed in 300 mM bicine, pH 8.0, 10 mM MgCl2, 0.1% SDS for 10 min at 25° C. Fluorescent signals were measured using a Scanarray 5000 (GSI Lumonics). ..

Article Title: Method of designing addressable array suitable for detection of nucleic acid sequence differences using ligase detection reaction
Article Snippet: .. Following hybridization, the arrays were washed in 300 mM bicine, pH 8.0, 10 mM MgCl2, 0.1% SDS for 10 min at 25° C. Fluorescent signals were measured using a Scanarray 5000 (GSI Lumonics). ..

Labeling:

Article Title: Bayesian Hierarchical Model for Estimating Gene Expression Intensity Using Multiple Scanned Microarrays
Article Snippet: .. The slides were scanned with ScanArray 5000 (GSI Lumonics) using an appropriate setting for samples labeled with Cy3 and Cy5. ..

Software:

Article Title: The excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis induces autophagy via the Sonic hedgehog pathway in mouse brain astrocytes
Article Snippet: .. The washed arrays were scanned using ScanArray 4000 (GSI Lumonics, UK), and the obtained data (16-bit tiff image) were analyzed by QuantArray software (GSI Lumonics, UK) by converting the signal intensity of each spot into text format. ..



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Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of <t>microarray</t> results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="250" height="auto" />
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Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of <t>microarray</t> results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="250" height="auto" />
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Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="100%" height="100%">

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

doi: 10.3389/fcimb.2021.624945

Figure Lengend Snippet: Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center.

Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

Techniques: Microarray, Isolation, Infection, Expressing, Labeling

Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

doi: 10.3389/fcimb.2021.624945

Figure Lengend Snippet: Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

Techniques: Quantitative RT-PCR, Microarray

A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( <xref ref-type=Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2. " width="100%" height="100%">

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

doi: 10.3389/fcimb.2021.624945

Figure Lengend Snippet: A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2.

Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

Techniques: Microarray