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g2565ca high‐resolution laser microarray scanner  (Agilent technologies)


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    Agilent technologies g2565ca high‐resolution laser microarray scanner
    G2565ca High‐Resolution Laser Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+microarray+laser+scanner/pmc07995094-182-15-20
    Average 90 stars, based on 1 article reviews
    g2565ca high‐resolution laser microarray scanner - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Cynomolgus macaques as a translational model of human immune responses to yellow fever 17D vaccination
    Article Snippet: Scanning of microarrays was performed with 3 μm resolution and 20-bit image depth, using a G2565CA high-resolution laser microarray scanner (Agilent Technologies).

    Article Title: Cynomolgus macaques as a translational model of human immune responses to yellow fever 17D vaccination.
    Article Snippet: Scanning of microarrays was performed with 3 μm resolution and 20-bit image depth, using a G2565CA high-resolution laser microarray scanner (Agilent Technologies).

    Microarray:

    Article Title: HOMEOBOX2, the paralog of SIX-ROWED SPIKE1/HOMEOBOX1, is dispensable for barley spikelet development
    Article Snippet: .. Finally, the chips were scanned at a high resolution of 2 μm using an Agilent DNA Microarray Scanner G2565CA. .. The resulting TIFF images were used to run batch extractions with the selection-appropriate grid using Agilent Feature Extraction Software v11.0.

    Article Title: Production of DHA and other LC PUFAs in plants
    Article Snippet: Slides were then washed at 37° C. for 1 minute using the Agilent GE Wash #1 followed by a second wash at 30° C. with Agilent GE Wash #2 for 1 minute and a final drying step using Nitrogen gas for 2 minutes and 30 seconds at 30° C. Slides were scanned immediately to minimize impact of environmental oxidants on signal intensities. .. Arrays were scanned using an Agilent G2565CA microarray scanner with SureScan high resolution technology (Agilent Technologies, Santa Clara, Calif.). ..

    Article Title: Changes in Gravitaxis and Gene-Expression in an Euglena gracilis Culture over Time
    Article Snippet: After the fragmentation of cRNA (about 600 ng of cRNA per sample), 40 μL of samples were loaded onto the arrays and hybridized in a hybridization oven (G2545A, Agilent Technologies, Santa Clara, CA, USA) for at least 17 h at 65 °C, at a rotational speed of 10 rpm. .. Subsequently, slides were washed with wash buffers and dried in an air stream for scanning with an Agilent C microarray scanner (G2565CA, Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Arabidopsis AGB1 participates in salinity response through bZIP17-mediated unfolded protein response
    Article Snippet: .. The Agilent DNA Microarray Scanner G2565CA and Agilent Feature Extraction 10.7.1.1 software detected the fluorescence signals. ..

    Article Title: Preclinical toxicity analyses of lentiviral vectors expressing the HIV-1 LTR-specific designer-recombinase Brec1
    Article Snippet: The labeling of aaUTP-cRNA was performed using Alexa Fluor 555 Reactive Dye (#A32756; Thermo Fisher Scientific) as recommended in the manual of the Amino Allyl MessageAmpTM II Kit (twofold downscaled reaction volumes). cRNA fragmentation, hybridization, and washing steps were carried out as recommended in the ‘One-Color Microarray-Based Gene Expression Analysis Protocol V5.7’, except that 500 ng of each fluorescently labeled cRNA population were used for hybridization. .. Slides were scanned using the Agilent Micro Array Scanner G2565CA (pixel resolution 3 μm, bit depth 20). ..

    Hybridization:

    Article Title: Mechanism Exploration on the Immunoregulation of Allogeneic Heart Transplantation Rejection in Rats With Exosome miRNA and Proteins From Overexpressed IDO1 BMSCs
    Article Snippet: Dephosphorylation and labeling reactions were performed using the miRNA Complete Labeling and Hyb Kit from Agilent. .. After hybridisation, washing, and drying the slides, the hybrid image was obtained using the Agilent chip scanner (G2565CA). .. The Agilent Feature Extraction (v10.7) software was employed to analyze the hybrid images and extract the data.

    Extraction:

    Article Title: Arabidopsis AGB1 participates in salinity response through bZIP17-mediated unfolded protein response
    Article Snippet: .. The Agilent DNA Microarray Scanner G2565CA and Agilent Feature Extraction 10.7.1.1 software detected the fluorescence signals. ..

    Software:

    Article Title: Arabidopsis AGB1 participates in salinity response through bZIP17-mediated unfolded protein response
    Article Snippet: .. The Agilent DNA Microarray Scanner G2565CA and Agilent Feature Extraction 10.7.1.1 software detected the fluorescence signals. ..

    Fluorescence:

    Article Title: Arabidopsis AGB1 participates in salinity response through bZIP17-mediated unfolded protein response
    Article Snippet: .. The Agilent DNA Microarray Scanner G2565CA and Agilent Feature Extraction 10.7.1.1 software detected the fluorescence signals. ..



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    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of <t>microarray</t> results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="250" height="auto" />
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    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of <t>microarray</t> results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="250" height="auto" />
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    Image Search Results


    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="100%" height="100%">

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center.

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Microarray, Isolation, Infection, Expressing, Labeling

    Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Quantitative RT-PCR, Microarray

    A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( <xref ref-type=Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2. " width="100%" height="100%">

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2.

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Microarray