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high microarray laser scanner  (Agilent technologies)


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    Agilent technologies high microarray laser scanner
    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of <t>microarray</t> results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="250" height="auto" />
    High Microarray Laser Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+microarray+laser+scanner/pmc07973279-312-16-22
    Average 90 stars, based on 1 article reviews
    high microarray laser scanner - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation"

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2021.624945

    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " title="... blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center.

    Techniques Used: Microarray, Isolation, Infection, Expressing, Labeling

    Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).
    Figure Legend Snippet: Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

    Techniques Used: Quantitative RT-PCR, Microarray

    A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( <xref ref-type=Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2. " title="... are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2.

    Techniques Used: Microarray

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    Hybridization:

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    Extraction:

    Article Title: Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
    Article Snippet: Finally, arrays were treated with the Stabilization and Drying Solution (Agilent Technologies) for 30 seconds at room temperature. .. Immediately after washing, slides were scanned with the Agilent Dual Laser Microarray Scanner and image data were read out and processed by the Feature Extraction v. 10 software (Agilent Technologies). .. The GeneSpring® 10 (Agilent Technologies) software was used to process the microarray data and to associate sample information.

    Software:

    Article Title: Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
    Article Snippet: Finally, arrays were treated with the Stabilization and Drying Solution (Agilent Technologies) for 30 seconds at room temperature. .. Immediately after washing, slides were scanned with the Agilent Dual Laser Microarray Scanner and image data were read out and processed by the Feature Extraction v. 10 software (Agilent Technologies). .. The GeneSpring® 10 (Agilent Technologies) software was used to process the microarray data and to associate sample information.



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    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of <t>microarray</t> results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="250" height="auto" />
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    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="100%" height="100%">

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center.

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Microarray, Isolation, Infection, Expressing, Labeling

    Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Quantitative RT-PCR, Microarray

    A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( <xref ref-type=Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2. " width="100%" height="100%">

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2.

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Microarray