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microarray dual channel laser scanner  (Danaher Inc)


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    Structured Review

    Danaher Inc microarray dual channel laser scanner
    Microarray Dual Channel Laser Scanner, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+microarray+laser+scanner/GenePix+Microarray+Systems/pmc02703804-173-10-17
    Average 94 stars, based on 460 article reviews
    microarray dual channel laser scanner - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Microarray:

    Article Title: Unraveling the role of HDAC3 as an immunotherapy prognostic biomarker and therapeutic target in advanced non-small cell lung cancer
    Article Snippet: The microarray was retrieved from − 80 °C storage and blocked with bovine serum albumin (BSA, 9048-46-8, Sigma) for 1.5 h. Following the blocking step, the microarray was incubated with plasma samples at a 1:1000 dilution for 1 h. After washing with 0.1% phosphate-buffered saline-Tween 20, the plates were incubated with Alexa Fluor 647 goat anti-human IgG (109-605-003, Jackson) diluted in 5% BSA. .. The microarray was then scanned using a GenePix 4300 A microarray scanner (141095, Molecular Devices) with a 635 nm excitation laser. .. Signal intensities of IgG for each protein were quantified using GenePix Pro v.6.0 software (Molecular Devices).

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Article Title: Supporting Information _ Lee and Fan _ Yale
    Article Snippet: .. An Axon Genepix microarray scanner 4000B (Molecular Devices, USA) was used to image DRAQ5-stained cells (emitting 635 nm) on the STRQNW arrays. ..

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    other:

    Article Title: Development of Lamprey-derived Antibodies Against Human Blood Group Antigens.
    Article Snippet: A major challenge in the glycosciences is the scarcity of sensitive and specific glycanbinding reagents, such as monoclonal antibodies, for detecting and isolating glycans.. Here we report the development and characterization of new monoclonal antibodies (mAbs) that bind carbohydrate-based red blood cell (RBC) antigens including the ABO(H) antigens.. This approach exploits the immune system of the sea lamprey (Petromyzon marinus), which strongly responds to human glycans to enable the generation of high affinity antibodies.

    Article Title: Development of lamprey-derived antibodies against human blood group antigens
    Article Snippet: The scans were analyzed using GenePix (Molecular Devices) software and GLAD (GLycan Array Dashboard) software ( ).

    Binding Assay:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Incubation:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Software:

    Article Title: Maackia amurensis seed lectin structure and sequence comparison with other M. amurensis lectins
    Article Snippet: MASL, MAL2, and MAA lectins were biotinylated and hybridized (30 ug/ml) to Glycan Array 300 arrays (RayBiotech) which contain 300 triplicated synthetic glycans along with biotinylated protein positive controls and empty negative controls. .. Lectin binding was detected by incubation with Cy5 equivalent dye-conjugated streptavidin and visualized with a GenePix 4000B microarray laser scanner equipped with GenePix Pro version 7.2 software ( https://genepix-pro.software.informer.com/7.2 ) (Molecular Devices). .. Mean signal intensities were normalized to positive controls and analyzed using RayBio GA-Glycan-300-SW Analysis software ( https://www.raybiotech.com/glycan-array-300-ga-glycan-300 ) (RayBiotech).

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Article Title: Biomarkers and immunogenic compositions for filarial parasites
    Article Snippet: Chips were air dried by centrifugation at 500×g for 10 min, stored in a light proof desiccator for at least 2 hr and scanned on a GENEPIX® 4300 with Autoloader (Molecular Devices) using the 635 nm laser. .. Resulting 16-bit TIFF images were quantified using GENEPIX® Pro Microarray Analysis Software (Molecular Devices) and a GENEPIX® Array List (GAL) file. ..

    Fluorescence:

    Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development
    Article Snippet: After being washed three times and air-dried through brief centrifugation, the slides were scanned using a Genepix 4200A scanner (Molecular Devices, CA, USA). .. The median foreground and background fluorescence intensities were measured for each spot on the protein microarrays using GenePix Pro 6.0 (Molecular Devices, CA, USA). ..



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    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of <t>microarray</t> results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="250" height="auto" />
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    Image Search Results


    Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( <xref ref-type=Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center. " width="100%" height="100%">

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: Down-regulation of distinct mRNAs coding for signature gamete-, ookinete- and oocyst-specific proteins in uis12(-) blood stage parasites. Shown are mean values of microarray results of total RNA isolated from mixed infected erythrocytes from uis12(-) (clone 1) - and WT- infected mice from two biological replicates. (A) Shown is a volcano-plot illustrating the fold change of the expression levels and the negative log p -values of all analyzed 2,890 P. berghei genes. The dotted black line represents a p -value of 0.05 and all transcripts with a p -value <0.05 are shown in red. Exemplary transcripts are highlighted and labeled in blue. (B) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among all transcripts (upper left), blood stage-specific transcripts (center), and gametocyte-specific transcripts (upper right) ( Otto et al., 2014 ). Exemplary transcripts are listed in the respective region of the chart. The number of transcripts analyzed is shown in a white circle inside the center. (C) Pie charts displaying the proportions of up- (green >2), non- (grey), and down- (orange <-2) regulated transcripts among female (left) and male (right) gametocyte-specific transcripts ( Yeoh et al., 2017 ). The number of transcripts analyzed is shown in a white circle inside the center.

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Microarray, Isolation, Infection, Expressing, Labeling

    Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: Confirmation of down-regulation of selected mRNAs by quantitative RT-PCR. Quantitative RT-PCR on selected blood stage and gametocyte transcripts validate the results of the microarray analysis. Shown are -fold changes of steady state mRNA levels of uis12(-) clone 1 compared to wild-type mixed blood stages for selected genes ( Puf1 , P28 , MDV , DOZI , Actin II , UIS1/IK2 , SET , MSP1 , and AMA1 ). qRT-PCR data were normalized to the steady state levels of HSP70 mRNA. The microarray data represent mean values of biological replicate 1 and 2 ( ± SD).

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Quantitative RT-PCR, Microarray

    A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( <xref ref-type=Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2. " width="100%" height="100%">

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Pleiotropic Roles for the Plasmodium berghei RNA Binding Protein UIS12 in Transmission and Oocyst Maturation

    doi: 10.3389/fcimb.2021.624945

    Figure Lengend Snippet: A motif is shared by up-regulated transcripts in uis12(-) parasites, and the comparison of dozi(-), cith(-) and uis12(-) target transcripts reveals possible overlapping functions. (A) Shown is a graphic display of the 10-nucleotide U-rich motif found in the ORF of transcripts that are up-regulated in absence of UIS12 in the microarray with biological replicate 1. The size of the depicted nucleotide represents its probability at the respective position. (B) Venn diagram comparing shared down-regulated transcripts of the gametocyte microarray analyses performed for dozi(-) and cith(-) with a -fold change lower than -1 ( Mair et al., 2010 ) and down-regulated transcripts in mixed blood stages of uis12(-) with a mean -fold change lower than -2.

    Article Snippet: Scanning of the microarrays was performed with 5 µm resolution and the extended mode using a ‘High Resolution Microarray Laser Scanner’ (G2505, Agilent Technologies).

    Techniques: Microarray