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Gemini Bio flow buffer
Flow Buffer, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+buffer/buffer+quench/pmc09383695-522-7-12
Average 86 stars, based on 1 article reviews
flow buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Magnetic Cell Separation:

Article Title: Impact of 3D cell culture hydrogels derived from basement membrane extracts or nanofibrillar cellulose on CAR-T cell activation
Article Snippet: .. Cells were centrifuged at 400 g for 4 minutes at 4 ◦ C and resuspended in MACS buffer (2% heat-inactivated FBS (Gemini Bio-Products) and 2 mM EDTA in PBS), followed by cell counting using a TC20 automated cell counter (Bio-Rad). .. CD4 + T cells were isolated using mouse CD4 (L3T4) microbeads (Miltenyi Biotec) according to the manufacturer’s protocol.

Article Title: Impact of 3D cell culture hydrogels derived from basement membrane extracts or nanofibrillar cellulose on CAR-T cell activation
Article Snippet: .. Cells were centrifuged at 400 g for 4 minutes at 4°C and resuspended in MACS buffer (2% heat-inactivated FBS (Gemini Bio-Products) and 2 mM EDTA in PBS), followed by cell counting using a TC20 automated cell counter (Bio-Rad). .. CD4 + T cells were isolated using mouse CD4 (L3T4) microbeads (Miltenyi Biotec) according to the manufacturer’s protocol.

Cell Counting:

Article Title: Impact of 3D cell culture hydrogels derived from basement membrane extracts or nanofibrillar cellulose on CAR-T cell activation
Article Snippet: .. Cells were centrifuged at 400 g for 4 minutes at 4 ◦ C and resuspended in MACS buffer (2% heat-inactivated FBS (Gemini Bio-Products) and 2 mM EDTA in PBS), followed by cell counting using a TC20 automated cell counter (Bio-Rad). .. CD4 + T cells were isolated using mouse CD4 (L3T4) microbeads (Miltenyi Biotec) according to the manufacturer’s protocol.

Article Title: Impact of 3D cell culture hydrogels derived from basement membrane extracts or nanofibrillar cellulose on CAR-T cell activation
Article Snippet: .. Cells were centrifuged at 400 g for 4 minutes at 4°C and resuspended in MACS buffer (2% heat-inactivated FBS (Gemini Bio-Products) and 2 mM EDTA in PBS), followed by cell counting using a TC20 automated cell counter (Bio-Rad). .. CD4 + T cells were isolated using mouse CD4 (L3T4) microbeads (Miltenyi Biotec) according to the manufacturer’s protocol.

Incubation:

Article Title: TRAF6 integrates innate immune signals to regulate glucose homeostasis via Parkin-dependent and Parkin-independent mitophagy.
Article Snippet: Mouse primary islet isolation and culture Mouse primary islets were isolated by perfusing pancreata with a solution of collagenase P (1 mg/ml; MilliporeSigma) in 1× Hanks’ balanced salt solution (HBSS; Sigma- Aldrich) into the pancreatic duct. .. Following excision, pancreata were incubated at 37°C for 13 min, and collagenase P was deactivated by addition of Quench Buffer [1× HBSS + 10% adult bovine serum (Gemini Bio)]. .. Pancreata were dissociated mechanically by vigorous shaking for 30 s, and the resulting cell suspension was passed through a 70 μM cell strainer (Thermo Fisher Scientific).

Article Title: TRAF6 integrates innate immune signals to regulate glucose homeostasis via Parkin-dependent and Parkin-independent mitophagy
Article Snippet: Mouse primary islets were isolated by perfusing pancreata with a solution of collagenase P (1 mg/ml; MilliporeSigma) in 1× Hanks’ balanced salt solution (HBSS; Sigma-Aldrich) into the pancreatic duct. .. Following excision, pancreata were incubated at 37°C for 13 min, and collagenase P was deactivated by addition of Quench Buffer [1× HBSS + 10% adult bovine serum (Gemini Bio)]. .. Pancreata were dissociated mechanically by vigorous shaking for 30 s, and the resulting cell suspension was passed through a 70 μM cell strainer (Thermo Fisher Scientific).



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Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Journal: STAR Protocols

Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

doi: 10.1016/j.xpro.2026.104471

Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

Techniques: Flow Cytometry, Isolation, Staining