flow cytometry staining buffer (Thermo Fisher)
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Thermo Fisher
flow cytometry staining buffer
Flow Cytometry Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+buffer/Matrix+DeepWell+Storage+Blocks/pmc13068824-328-4-9
Average 97 stars, based on 1 article reviews
Flow Cytometry Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+buffer/Matrix+DeepWell+Storage+Blocks/pmc13068824-328-4-9
Average 97 stars, based on 1 article reviews
flow cytometry staining buffer - by Bioz Stars,
2026-09
97/100 stars
Images
1) Product Images from "Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system"
Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system
Journal: STAR Protocols
doi: 10.1016/j.xpro.2026.104471
Figure Legend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Techniques Used: Flow Cytometry, Isolation, Staining
Related Articles
Staining:Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: The CD34 + fraction was stained with the following antibodies (lineage cocktail on APC) in Flow Cytometry Staining Buffer (ThermoFisher Cat# 00‐4222‐26): APC anti‐human CD2 antibody (clone RPA‐2.10, Biolegend Cat# 300213), APC anti‐human CD3 antibody (clone HIT3, Biolegend Cat# 300311), APC anti‐human CD14 antibody (clone M5E2, Biolegend Cat# 301807), APC anti‐human CD16 antibody (clone 3G8, Biolegend Cat# 302011), APC anti‐human CD56 antibody (clone HCD56, Biolegend Cat# 318309), APC anti‐human CD235ab antibody (clone HIR2, Biolegend Cat# 306607), Alexa Fluor® 700 anti‐human CD34 antibody (clone 561, Biolegend Cat# 343621), PE anti‐human CD45RA antibody (clone HI100, Biolegend Cat# 304107), PE/Cy7 anti‐human CD10 antibody (clone eBIOCB‐CALLA, ThermoFisher Cat# 25‐0106‐42), BrilliantVioletTM 421 anti‐human CD90 (clone 5E10, Biolegend Cat# 328121), BrilliantVioletTM 510 anti‐human CD38 (clone HB7, Biolegend Cat# 356633), and BrilliantVioletTM 605 anti‐human CD19 (clone HIB19, Biolegend Cat# 302243). .. The CD34 − fraction was stained with the following antibodies in Article Title: Ts -Hsp70 confers dual-stage protective immunity against Trichinella spiralis infection by sustaining M1 macrophage polarization in mice Article Snippet: .. After supernatant removal, pellets were resuspended in 2 mL Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: .. FLs were dissociated in Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system Article Snippet: .. Note: We use commercial Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: FLs were dissociated in Flow Cytometry Staining Buffer (ThermoFisher Cat# 00‐4222‐26) using a 21G × 15 mm needle attached to a syringe (BD Microlance Cat# 10472204‐X and 3000185) and further CD34‐enriched using the CD34 MicroBead Kit Ultrapure human (Millitenyi Cat# 130‐100‐453) according to the manufacturer's instructions. .. The CD34 + fraction was stained with the following antibodies (lineage cocktail on APC) in Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: PER‐494 (CVCL_A8JU), RS4;11 (ATCC®CRL‐1873TM), SEM (DSMZ ACC 546), Nalm6 (ATCC®CRL‐3273TM), MV4;11 (ATCC®CRL‐9591TM), MOLM‐13 (DSMZ ACC 554), THP‐1 (ATCC®TIB‐200TM), Nomo‐1 (DSMZ ACC 542), and Kasumi1 (ATCC®CRL‐2724TM) cells were maintained in 20% fetal calf serum (FCS) 1% P/S 1% l ‐glut RPMI (Roswell Park Memorial Institute) 1640 (cells kindly provided by Professor Mark Dawson, Professor Brian Huntly, and Dr. Rishi Sury Kotecha). .. Dissociated FL cells were stained using the following antibody mix in Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: Lineage depletion was performed on dissociated FL cells using the EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies Cat# 19856) according to the manufacturer's instructions. .. Cells were stained using the following antibody mix in Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system Article Snippet: ArCTM Amine Reactive Compensation , eBioscience , Cat#A10346. .. Flow Cytometry:Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: The CD34 + fraction was stained with the following antibodies (lineage cocktail on APC) in Flow Cytometry Staining Buffer (ThermoFisher Cat# 00‐4222‐26): APC anti‐human CD2 antibody (clone RPA‐2.10, Biolegend Cat# 300213), APC anti‐human CD3 antibody (clone HIT3, Biolegend Cat# 300311), APC anti‐human CD14 antibody (clone M5E2, Biolegend Cat# 301807), APC anti‐human CD16 antibody (clone 3G8, Biolegend Cat# 302011), APC anti‐human CD56 antibody (clone HCD56, Biolegend Cat# 318309), APC anti‐human CD235ab antibody (clone HIR2, Biolegend Cat# 306607), Alexa Fluor® 700 anti‐human CD34 antibody (clone 561, Biolegend Cat# 343621), PE anti‐human CD45RA antibody (clone HI100, Biolegend Cat# 304107), PE/Cy7 anti‐human CD10 antibody (clone eBIOCB‐CALLA, ThermoFisher Cat# 25‐0106‐42), BrilliantVioletTM 421 anti‐human CD90 (clone 5E10, Biolegend Cat# 328121), BrilliantVioletTM 510 anti‐human CD38 (clone HB7, Biolegend Cat# 356633), and BrilliantVioletTM 605 anti‐human CD19 (clone HIB19, Biolegend Cat# 302243). .. The CD34 − fraction was stained with the following antibodies in Article Title: Ts -Hsp70 confers dual-stage protective immunity against Trichinella spiralis infection by sustaining M1 macrophage polarization in mice Article Snippet: .. After supernatant removal, pellets were resuspended in 2 mL Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: .. FLs were dissociated in Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system Article Snippet: .. Note: We use commercial Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: FLs were dissociated in Flow Cytometry Staining Buffer (ThermoFisher Cat# 00‐4222‐26) using a 21G × 15 mm needle attached to a syringe (BD Microlance Cat# 10472204‐X and 3000185) and further CD34‐enriched using the CD34 MicroBead Kit Ultrapure human (Millitenyi Cat# 130‐100‐453) according to the manufacturer's instructions. .. The CD34 + fraction was stained with the following antibodies (lineage cocktail on APC) in Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: PER‐494 (CVCL_A8JU), RS4;11 (ATCC®CRL‐1873TM), SEM (DSMZ ACC 546), Nalm6 (ATCC®CRL‐3273TM), MV4;11 (ATCC®CRL‐9591TM), MOLM‐13 (DSMZ ACC 554), THP‐1 (ATCC®TIB‐200TM), Nomo‐1 (DSMZ ACC 542), and Kasumi1 (ATCC®CRL‐2724TM) cells were maintained in 20% fetal calf serum (FCS) 1% P/S 1% l ‐glut RPMI (Roswell Park Memorial Institute) 1640 (cells kindly provided by Professor Mark Dawson, Professor Brian Huntly, and Dr. Rishi Sury Kotecha). .. Dissociated FL cells were stained using the following antibody mix in Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: Lineage depletion was performed on dissociated FL cells using the EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies Cat# 19856) according to the manufacturer's instructions. .. Cells were stained using the following antibody mix in Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system Article Snippet: ArCTM Amine Reactive Compensation , eBioscience , Cat#A10346. .. Formulation:Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system Article Snippet: .. Note: We use commercial Binding Assay:Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system Article Snippet: .. Note: We use commercial Recombinase Polymerase Amplification:Article Title: A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP‐ALL uncovers novel therapeutic targets Article Snippet: FLs were dissociated in Flow Cytometry Staining Buffer (ThermoFisher Cat# 00‐4222‐26) using a 21G × 15 mm needle attached to a syringe (BD Microlance Cat# 10472204‐X and 3000185) and further CD34‐enriched using the CD34 MicroBead Kit Ultrapure human (Millitenyi Cat# 130‐100‐453) according to the manufacturer's instructions. .. The CD34 + fraction was stained with the following antibodies (lineage cocktail on APC) in |