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facs buffer supplemented 1 µg/ml dapi  (Becton Dickinson)

 
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    Becton Dickinson facs buffer supplemented 1 µg/ml dapi
    Facs Buffer Supplemented 1 µg/Ml Dapi, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer+dapi/facs+buffer+supplemented+1+%C2%B5g+ml+dapi/pmc08662543-399-12-22
    Average 90 stars, based on 1 article reviews
    facs buffer supplemented 1 µg/ml dapi - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    FACS:

    Article Title: Pharmacological Activation of cGAS for Cancer Immunotherapy
    Article Snippet: The remaining tumor cells were washed and diluted to a concentration of 1 × 10 7 cells/mL in FACS buffer supplemented with 50 nM dasatinib, and the cell suspension was aliquoted into a 96-well plate (REF 655180; Greiner Bio-One). .. Cells were washed twice, suspended in FACS buffer supplemented with 1 μg/mL DAPI, and then analyzed using a 5-laser LSRII flow cytometer (BD). .. After another wash with FACS buffer supplemented with 50 nM dasatinib, the plated cells were incubated with Fc-block (anti-CD16/CD32, Clone 2.4G2; Tonbo) for 15 minutes at 4°C.

    Flow Cytometry:

    Article Title: Pharmacological Activation of cGAS for Cancer Immunotherapy
    Article Snippet: The remaining tumor cells were washed and diluted to a concentration of 1 × 10 7 cells/mL in FACS buffer supplemented with 50 nM dasatinib, and the cell suspension was aliquoted into a 96-well plate (REF 655180; Greiner Bio-One). .. Cells were washed twice, suspended in FACS buffer supplemented with 1 μg/mL DAPI, and then analyzed using a 5-laser LSRII flow cytometer (BD). .. After another wash with FACS buffer supplemented with 50 nM dasatinib, the plated cells were incubated with Fc-block (anti-CD16/CD32, Clone 2.4G2; Tonbo) for 15 minutes at 4°C.



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    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and <t>DAPI</t> (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).
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    Image Search Results


    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

    Journal: bioRxiv

    Article Title: Inhibiting SOX18 with propranolol restores vascular integrity in NR2F2-driven malformations

    doi: 10.1101/2025.04.25.650344

    Figure Lengend Snippet: A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

    Article Snippet: Cells were then washed with FACS buffer (1:5), and resuspended in 300μl FACS buffer + DAPI ((Miltenyi Biotec, # 130-111-570) for live/dead cell identification.

    Techniques: Immunostaining, Expressing, Staining