facs buffer (Cell Signaling Technology Inc)
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98
Structured Review
Cell Signaling Technology Inc
facs buffer
Facs Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+buffer+dapi/DAPI/pm40316023-209-7-14
Average 98 stars, based on 2155 article reviews
Facs Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+buffer+dapi/DAPI/pm40316023-209-7-14
Average 98 stars, based on 2155 article reviews
facs buffer - by Bioz Stars,
2026-09
98/100 stars
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Incubation:Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: Sections were then incubated with primary antibodies against UCP1 (Abcam, ab10983), PPARγ (Santa Cruz Biotechnology, sc-7273), and PDGFRα (R&D Systems, AF-307-NA) at 4 °C overnight. .. After washing, sections were incubated with appropriate fluorophore-conjugated secondary antibodies for 1 h at room temperature and counterstained with Article Title: Investigating the Effect and Mechanism of Protocatechuic Aldehyde on Vascular Dementia Based on Multi-Omics Approach. Article Snippet: .. The 300 μL blocking solution was added and blocked at 25 ◦C for 1 h. The slides were placed in primary antibodies, including monocarboxylate Transporter 1 [MCT1], MCT2 (PA5-72957, PA5-76603, Thermo Fisher Scientific, Pittsburgh, PA, USA), MCT4, GFAP, NeuN (22787-1-AP, 60190-1-Ig, 26975-1-AP, Proteintech, Chicago, IL, USA), Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Blocking Assay:Article Title: Investigating the Effect and Mechanism of Protocatechuic Aldehyde on Vascular Dementia Based on Multi-Omics Approach. Article Snippet: .. The 300 μL blocking solution was added and blocked at 25 ◦C for 1 h. The slides were placed in primary antibodies, including monocarboxylate Transporter 1 [MCT1], MCT2 (PA5-72957, PA5-76603, Thermo Fisher Scientific, Pittsburgh, PA, USA), MCT4, GFAP, NeuN (22787-1-AP, 60190-1-Ig, 26975-1-AP, Proteintech, Chicago, IL, USA), Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Staining:Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL |
