facs buffer with dapi (Thermo Fisher)

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Thermo Fisher facs buffer with dapi
Facs Buffer With Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+buffer+dapi/dapi/pmc12036604-901-18-27
Average 90 stars, based on 1 article reviews

facs buffer with dapi - by Bioz Stars, 2026-09

90/100 stars

Images

Immunocytochemistry:

Article Title: Neuron-derived extracellular vesicles in plasma present a potential non-invasive biomarker for Huntingtin protein and RNA assessment in Huntington disease
Article Snippet: .. NSCs cultures were characterized by ICC using the markers Nestin 10C2 (StemCell, 60091.1, 1:1000), Sox1 JJ20-40 (Thermo Scientific, MA532447, 1:1000) and Pax6 Poly19013 (BioLegend, 901301, 1:1000), and absence of the pluripotency marker Oct4 3A2A20 (StemCell, 60093.1, 1:1000) in the two lines. iPSC-differentiated neurons were stained for DARPP-32 19A3 (Cell signaling, 2306S, 1:1000), NeuN clone A60 (Millipore, mab377), β3-Tubulin D71G9 (Cell signaling, 5568S, 1:1000), MAP2 (Abcam, ab32454, 1:1000) and counterstained with DAPI to reveal nuclei (Thermo Scientific, D1306). ..

Marker:

Staining:

Article Title: Microwave-assisted immunostaining for rapid labeling of matrix-embedded multicellular structures
Article Snippet: A solution containing Alexa Fluor 488-conjugated anti-Lamin B1 (1:25; ab194106, Abcam, Eugene, OR), Alexa Fluor 555-conjugated anti-GM130 (1:10; Catalog #560066, BD Biosciences, Franklin Lakes, NJ), and Alexa Fluor 647-conjugated anti-β-Catenin (L54E2) (1:10; Catalog #4627, Cell Signaling Technology, Danvers, MA) in blocking buffer was added to the samples and microwaved at the indicated times. .. Nuclear staining was performed with a large volume of DAPI (500 μ l; 10 μ g/ml; ThermoFisher) in DPBS +/+ with 0.2% TX-100 for 10 min. .. Following nuclear staining, samples were placed in large dishes (100 mm cell culture dishes, Fisher Scientific) and completely immersed in about 150 ml of DPBS +/+ with 0.2% TX-100 to wash samples.

Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair
Article Snippet: .. The cytoskeleton and nuclei were stained with rhodamine (Solarbio, China) and DAPI (Thermo Fisher, USA). .. The uptake of 2D-sEVs and 3D-sEVs was observed via confocal laser scanning microscopy (Carl Zeiss, Germany).

Article Title: Palmitic acid reduces LDLR-dependent uptake of macrophage-derived extracellular vesicles by hepatoma cells
Article Snippet: .. Nuclei were stained with 4′,6‐diamidino‐2‐phenylindole (DAPI) (Thermo Fisher Scientific), and images were acquired using a Zeiss 880 Airyscan FAST confocal microscope (Carl Zeiss), under 40× and 63 × magnification. ..

Blocking Assay:

Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair
Article Snippet: .. The cells were then blocked with normal goat serum at room temperature for 30 min. After blocking, the cells were incubated with primary antibodies specific for COL-1 (Proteintech, USA) for BMSCs and VEGF (Proteintech, USA) for HUVECs at 4 °C for 8–16 h. Following three washes with PBS, the cells were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA) in the dark for 1 h. The nuclei were counterstained with DAPI (Thermo Fisher, USA) in the dark for 5 min. Fluorescence images were acquired via a confocal microscope (Carl Zeiss, Germany). .. SD rats (male, 10 weeks old, approximately 250 g) were purchased from the Guangdong Medical Laboratory Animal Center.

Incubation:

Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair
Article Snippet: .. The cells were then blocked with normal goat serum at room temperature for 30 min. After blocking, the cells were incubated with primary antibodies specific for COL-1 (Proteintech, USA) for BMSCs and VEGF (Proteintech, USA) for HUVECs at 4 °C for 8–16 h. Following three washes with PBS, the cells were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA) in the dark for 1 h. The nuclei were counterstained with DAPI (Thermo Fisher, USA) in the dark for 5 min. Fluorescence images were acquired via a confocal microscope (Carl Zeiss, Germany). .. SD rats (male, 10 weeks old, approximately 250 g) were purchased from the Guangdong Medical Laboratory Animal Center.

Article Title: MitoQ Protects Against Oxidative Stress-Induced Mitochondrial Dysregulation in Human Cardiomyocytes
Article Snippet: Cardiomyocytes were then incubated with Protein block (Dako, Victoria, Australia) to prevent unspecific antibody binding followed by Cardiac Troponin-T (cTnT 1:500; mouse polyclonal antibody; Cat# ab45932, Abcam) and Anti-Hsp60 (1:300; rabbit polyclonal antibody; Cat# ab46798, Abcam) overnight incubation at 4 °C. .. Cardiomyocytes were then incubated with Alexa Fluor 594 (1:200; goat anti-mouse secondary antibody; Cat# A20004, Thermo Fisher), Alexa Fluor 488 (1:200; goat anti-rabbit secondary antibody; Cat# A20000, Thermo Fisher) and DAPI (1:200; nuclear counterstain; Cat# 62248, Thermo Fisher) for 1 h at 25 °C. .. Coverslips containing cardiomyocytes were mounted onto glass slides using a fluorescence mounting medium (Cat# S3023, Agilent Dako) and left to dry overnight at 4 °C.

Fluorescence:

Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair
Article Snippet: .. The cells were then blocked with normal goat serum at room temperature for 30 min. After blocking, the cells were incubated with primary antibodies specific for COL-1 (Proteintech, USA) for BMSCs and VEGF (Proteintech, USA) for HUVECs at 4 °C for 8–16 h. Following three washes with PBS, the cells were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA) in the dark for 1 h. The nuclei were counterstained with DAPI (Thermo Fisher, USA) in the dark for 5 min. Fluorescence images were acquired via a confocal microscope (Carl Zeiss, Germany). .. SD rats (male, 10 weeks old, approximately 250 g) were purchased from the Guangdong Medical Laboratory Animal Center.

Microscopy:

Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair
Article Snippet: .. The cells were then blocked with normal goat serum at room temperature for 30 min. After blocking, the cells were incubated with primary antibodies specific for COL-1 (Proteintech, USA) for BMSCs and VEGF (Proteintech, USA) for HUVECs at 4 °C for 8–16 h. Following three washes with PBS, the cells were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA) in the dark for 1 h. The nuclei were counterstained with DAPI (Thermo Fisher, USA) in the dark for 5 min. Fluorescence images were acquired via a confocal microscope (Carl Zeiss, Germany). .. SD rats (male, 10 weeks old, approximately 250 g) were purchased from the Guangdong Medical Laboratory Animal Center.

other:

Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets
Article Snippet: DAPI (ThermoFisher; D1306) was used for nuclear staining.

Enzyme-linked Immunosorbent Assay:

Article Title: HydroWrap for T2DM-Related Fractures: A smart H 2 S-delivery controller modulating Macrophage senescence
Article Snippet: Antibodies targeting β-actin, p65, p-p65, Parkin, p62, and LC3B, along with goat anti-rabbit IgG-HRP and goat anti-mouse IgG-HRP, were obtained from Cell Signaling Technology (CST, USA) and GeneTex (USA). .. MitoTracker, phalloidin, and DAPI were provided by ThermoFisher (USA), and ELISA kits were sourced from Elabscience Biotechnology (Wuhan, China). ..

Review