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dntp mixture  (TaKaRa)


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    Structured Review

    TaKaRa dntp mixture
    Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 8908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dntps/dNTP+Mixture/pm42884072-243-15-20
    Average 96 stars, based on 8908 article reviews
    dntp mixture - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Elucidating Temporally Ordered Physiological Events During Liver Regeneration via Integrated Transcriptomic-Histological Profiling.
    Article Snippet: After DNase I digestion, we reverse-transcribed 500 ng of total RNA with SuperScript VILO Master Mix (Invitrogen). .. We used reverse-transcribed cDNA as the template for gene-expression analysis with 10× Ex Taq Buffer, dNTP Mixture, and Ex Taq (Takara Bio Inc., Kusatsu, Japan) according to the manufacturer’s instructions. ..

    Analysis:

    Article Title: Elucidating Temporally Ordered Physiological Events During Liver Regeneration via Integrated Transcriptomic-Histological Profiling.
    Article Snippet: After DNase I digestion, we reverse-transcribed 500 ng of total RNA with SuperScript VILO Master Mix (Invitrogen). .. We used reverse-transcribed cDNA as the template for gene-expression analysis with 10× Ex Taq Buffer, dNTP Mixture, and Ex Taq (Takara Bio Inc., Kusatsu, Japan) according to the manufacturer’s instructions. ..

    Polymerase Chain Reaction:

    Article Title: Abyssal–hadal cumaceans (Crustacea: Peracarida: Cumacea) exhibit depth-related patterns in species composition across the Japan and Kuril–Kamchatka Trenches
    Article Snippet: .. PCR was performed in 25 μL reaction volumes containing 2.5 μL DNA extract, 2.5 μL PCR buffer, 2.0 μL dNTP mixture, 0.3 μL forward and reverse primers (20 μM each), and 0.1 μL Ex Taq Hot Start Version (TaKaRa Bio, Shiga, Japan). .. The cycling conditions were as follows: initial denaturation at 94 ◦ C for 120 s, followed by 35 cycles of denaturation at 98 ◦ C for 30 s, annealing at 42–45 ◦ C (both COI and 16S) for 40 s, and extension at 72 ◦ C for 60 s. The PCR products were purified using ExoSAP-IT Express (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's protocol.

    Article Title: Effects of vineyard inter-row grass cover treatment on microbial diversity and metabolite profiles during Cabernet Sauvignon fermentation
    Article Snippet: .. The 50 μL PCR reaction system contained 1 μL DNA template, 2 μL of each forward and reverse primer (10 μmol/L), 4 μL dNTP mixture (2.5 mmol/L), 5 μL 10× Pyrobest Buffer (Takara, Japan), 0.3 μL Pyrobest DNA Polymerase (2.5 U/μL, Takara, Japan), and 35.7 μL ddH2O. ..

    Article Title: Multidrug resistance, extended-spectrum β-lactamase (ESBL) production, and colistin resistance in Escherichia coli from retail chicken meat in fresh markets in Bangkok, Thailand
    Article Snippet: Contamination of chicken meat with antimicrobial-resistant bacteria, particularly multidrug-resistant (MDR), colistin-resistant, and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli), threatens its safety for human consumption.. This study was conducted to determine the prevalence and antimicrobial resistance (AMR) profiles, particularly ESBL production and colistin resistance in E. coli isolated from retail chicken meat in fresh markets in Bangkok, Thailand.. A total of 200 (81.6%) E. coli isolates were recovered from 245 chicken meat samples collected from fresh markets across six regions of Bangkok.

    Article Title: First insight into the chemosynthetic bacterial symbiosis in the endangered bivalve Pegophysema bialata (Lucinidae: Pegophyseminae)
    Article Snippet: .. The PCR mixture consisted of 17.5 μL distilled water, 2.5 μL EX Taq buffer (10×), 2.0 μL dNTP mixture (2.5 mM each), 0.13 μL Ex Taq Hot Start Version (TaKaRa Bio, Shiga, Japan), 0.3 μL of each forward and reverse primer (20 μM), and 2.5 μL genomic DNA. ..

    Concentration Assay:

    Article Title: A rapid, inexpensive diagnostic for bacterial pathogen and resistance detection in resource-limited settings.
    Article Snippet: RPA reactions were set up in 0.2- ml PCR strip tubes using the TwistAmp Liquid Basic Kit (TwistDx). .. Each 250 μl reaction mix contained 10 μl of diluted and lysed bacterial template, 165 μl of TwistAmp 2× buffer (to yield a final 0.8× concentration), 22 μl of TwistAmp 20× Core Reaction Mix (1× final concentration), 44 μl of TwistAmp 10× E- Mix (1× final concentration), and 44 μl of a 4× deoxynucleoside triphosphate (dNTP) mix (containing 2.5 μM each dNTP; Takara Biosciences) to achieve a final dNTP concentration of 1 μM. .. Reactions were mixed gently by pipetting and incubated at 37°C for 50 min. Amplification products were purified using QIAquick column purification (QIAGEN) and visualized using Invitrogen E- Gel General Purpose Agarose Gels (2%) (Thermo Fisher Scientific), precast with SYBR Safe DNA stain.

    Amplification:

    Article Title: Multidrug resistance, extended-spectrum β-lactamase (ESBL) production, and colistin resistance in Escherichia coli from retail chicken meat in fresh markets in Bangkok, Thailand
    Article Snippet: Contamination of chicken meat with antimicrobial-resistant bacteria, particularly multidrug-resistant (MDR), colistin-resistant, and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli), threatens its safety for human consumption.. This study was conducted to determine the prevalence and antimicrobial resistance (AMR) profiles, particularly ESBL production and colistin resistance in E. coli isolated from retail chicken meat in fresh markets in Bangkok, Thailand.. A total of 200 (81.6%) E. coli isolates were recovered from 245 chicken meat samples collected from fresh markets across six regions of Bangkok.

    Recombinant:

    Article Title: Inception-level signal amplification: Cascaded DNAzyme-Cas9 nickase achieves sub-nanomolar kanamycin tracking.
    Article Snippet: Background: Kanamycin's persistent contamination in agricultural products poses significant human health risks due to its nephrotoxicity and bioaccumulation via the food chain.. Existing methods for on-site kanamycin monitoring lack sufficient sensitivity and portability, limiting their utility in field settings.. The urgent need for rapid detection technologies remains unmet.

    Article Title: C1orf35 contributes to high anabolic metabolism by simultaneously promoting aerobic glycolysis and oxidative phosphorylation in multiple myeloma cells.
    Article Snippet: Similar to other malignancies, multiple myeloma (MM) has acquired several functional capabilities known as “Hallmarks of cancer”, and one of them is the deregulation of cell metabolism, especially glucose metabolism.. Our current study focuses on the role of Chromosome 1 Open Reading Frame 35(C1orf35) in the glucose metabolism of MM cells.. We found that the expression of C1orf35 was negative correlated with the overall survival of MM patients, MM cell lines with high C1orf35 expression not only had a faster proliferation rate but also higher levels of both aerobic glycolysis and oxidative phosphorylation (OXPHOS).

    Gene Therapy:

    Article Title: C1orf35 contributes to high anabolic metabolism by simultaneously promoting aerobic glycolysis and oxidative phosphorylation in multiple myeloma cells.
    Article Snippet: Similar to other malignancies, multiple myeloma (MM) has acquired several functional capabilities known as “Hallmarks of cancer”, and one of them is the deregulation of cell metabolism, especially glucose metabolism.. Our current study focuses on the role of Chromosome 1 Open Reading Frame 35(C1orf35) in the glucose metabolism of MM cells.. We found that the expression of C1orf35 was negative correlated with the overall survival of MM patients, MM cell lines with high C1orf35 expression not only had a faster proliferation rate but also higher levels of both aerobic glycolysis and oxidative phosphorylation (OXPHOS).



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