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sanger dideoxynucleotide chain termination (Sangon Biotech)

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Sangon Biotech sanger dideoxynucleotide chain termination
Sanger Dideoxynucleotide Chain Termination, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dideoxynucleotide+terminators/dideoxynucleotide+chain+termination+method/pmc11406935-101-17-22
Average 86 stars, based on 1 article reviews

sanger dideoxynucleotide chain termination - by Bioz Stars, 2026-09

86/100 stars

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Amplification:

Article Title: Novel isolates of hydrogen-oxidizing chemolithoautotrophic Sulfurospirillum provide insight to the functions and adaptation mechanisms of Campylobacteria in shallow-water hydrothermal vents
Article Snippet: .. The 16S rRNA gene was amplified via PCR using primers Eubac 27F/1492R ( ) and sequenced using Sanger dideoxynucleotide chain-termination method by Sangon Biotech Co. Ltd. (Shanghai, China). ..

Polymerase Chain Reaction:

Article Title: Analysis of the relationship between invasive capability of Helicobacter pylori and gastroduodenal diseases.
Article Snippet: .. 115 The PCR products of cagA 3 ́region were sequenced by the fluorescent dideoxy terminator method 116 (Sangon Biotech Co. Ltd., China). .. The sequenced results were matched against nucleotide 117 sequences presented in GeneBank using the BLASTN program, and then analyzed cagA-EPIYA 118 with Bioedit software.

other:

Article Title: Reference gene selection for qRT-PCR assays in Stellera chamaejasme subjected to abiotic stresses and hormone treatments based on transcriptome datasets
Article Snippet: To further confirm that the amplicon corresponded to the target sequence, PCR products contained in the agarose gel were extracted using a TIANgel Midi Purification Kit (TIANGEN, Beijing, China) and then sequenced using the dideoxy chain-termination method by Sangon Biotech (Shanghai, China) Co., Ltd. qRT-PCR reactions were carried out with the Fast Start Universal SYBR GreenMaster (Roche, Basel, Switzerland) on a Bio-Rad CFX96 Real-Time PCR system (Bio-Rad, Hercules, CA, USA) in accordance with the manufacturer’s instructions.

Article Title: Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China
Article Snippet: The PCR amplification products of suspected positive cases were purified and sequenced on both strands using the dideoxy-terminal method in Sangon Biotech Company (Shanghai, China).

Article Title: Molecular characterization and phylogenetic analyses of MetAP2 gene and protein of Nosema bombycis isolated from Guangdong, China
Article Snippet: Following PCR verification using the primers MC-F/R, the samples were sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing using the dideoxy method.

Article Title: Isolation and Characterization of Cold-Adapted PGPB and Their Effect on Plant Growth Promotion
Article Snippet: The PCR products were checked by 1% agarose gel electrophoresis and were subsequently sequenced using the dideoxy chain-termination method at Sangon Biotech Co., Ltd. (China).

Article Title: Novel isolates of hydrogen-oxidizing chemolithoautotrophic Sulfurospirillum provide insight to the functions and adaptation mechanisms of Campylobacteria in shallow-water hydrothermal vents.
Article Snippet: The 16S rRNA gene was amplified via PCR using primers Eubac 27F/1492R (49) and sequenced using Sanger dideoxynucleotide chain-termination method by Sangon Biotech Co. Ltd. (Shanghai, China).

Sequencing:

Article Title: Cloning, 3D Modeling and Expression Analysis of Three Vacuolar Invertase Genes from Cassava ( Manihot Esculenta Crantz)
Article Snippet: The PCR products were purified from a 1% agar gel and cloned in the pMD18-T vector (TaKaRa). .. Sequencing was performed using the dideoxynucleotide chain termination method (Sangon Biological Engineering Technology and Services Company, Shanghai, China). .. Multiple sequence alignments, isoelectric point and molecular weight of the deduced vacuolar invertase protein were analyzed using the DNAman 6.0 program (Lynnon Biosoft, Quebec, QC, Canada).