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dideoxynucleotide chain termination method  (TaKaRa)


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    TaKaRa dideoxynucleotide chain termination method
    Dideoxynucleotide Chain Termination Method, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dideoxynucleotide+terminators/pmc03316592-146-6-10
    Average 86 stars, based on 1 article reviews
    dideoxynucleotide chain termination method - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Recombinant:

    Article Title: Molecular characters and expression analysis of the gene encoding eclosion hormone from the Asian corn borer, Ostrinia furnacalis.
    Article Snippet: Using rapid amplification of cDNA ends (RACE), the cDNA encoding eclosion hormone (EH) was cloned from the brain of Ostrinia furnacalis.. The full Osf-EH cDNA is 986 bp and contains a 267 bp open reading frame encoding an 88 amino acid preprohormone, which including a hydrophobic 26 amino acid signal peptide and a 62 amino acid mature peptide.. The mature Osf-EH shows high identity with Manduca sexta (95.2%), Helicoverpa armigera (91.9%) and Bombyx mori (85.5%), but low identify with Tribolium castaneum (63.6%), Drosophila melanogaster (56.5%) and Apis mellifera (54.8%).

    Article Title: Characterization of the complete mitochondrial genome of the giant silkworm moth, Eriogyna pyretorum (Lepidoptera: Saturniidae)
    Article Snippet: The purified PCR product was ligated into T-vector (TaKaRa Co., Dalian, China) and then transformed into XL-1 blue competent bacteria according to the method of Wei et al. (2008) . .. The positive recombinant clone with an insert was sequenced using the dideoxynucleotide chain termination method (TaKaRa Co., Dalian, China) at least three times. ..

    Article Title: The complete nucleotide sequence of the mitochondrial genome of Phthonandria atrilineata (Lepidoptera: Geometridae).
    Article Snippet: Using long-polymerase chain reaction (LongPCR) method, we determined the complete nucleotide sequence of the mitochondrial genome (mitogenome) of Phthonandria atrilineata.. The complete mtDNA from P. atrilineata was 15,499 base pairs in length and contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region.. The P. atrilineata genes were in the same order and orientation as the completely sequenced mitogenomes of other lepidopteran species.

    Clone Assay:

    Article Title: Identification and Molecular Characterization of a Chitin-Binding Protein from the Beet Webworm, Loxostege sticticalis L.
    Article Snippet: .. Positive cDNA clones were sequenced using the dideoxynucleotide chain termination method (Takara Co., Dalian, China), and the sequences from 282 clones were submitted to BLAST analysis in GenBank. ..

    Article Title: Molecular and biochemical characterization of juvenile hormone epoxide hydrolase from the silkworm, Bombyx mori.
    Article Snippet: One major route of insect juvenile hormone (JH) degradation is epoxide hydration by JH epoxide hydrolase (JHEH).. A fulllength cDNA (1536 bp) encoding a microsomal JHEH was isolated from the silkworm, Bombyx mori.. Bommo-JHEH cDNA contains an open reading frame encoding a 461-amino acid protein (52 kDa), which reveals a high degree of similarity to the previously reported insect JHEHs.

    Article Title: Structural organization and expression analysis of the cDNA encoding allatotropin in the cotton bollworm, Helicoverpa armigera.
    Article Snippet: The cDNA encoding allatotropin (AT), which stimulates juvenile hormone (JH) biosynthesis by the adult corpora allata (CA) of Lepidoptera, was cloned from Helicoverpa armigera (Har).. Har-AT cDNA encodes a 135–amino acid polypeptide precursor containing a 13–amino acid AT sequence identical to Manduca sexta AT (Mas-AT).. Three other H. armigera AT cDNAs encoding 172, 213, and 246 amino acids were also cloned.

    Article Title: Functional Analysis of General Odorant Binding Protein 2 from the Meadow Moth, Loxostege sticticalis L. (Lepidoptera: Pyralidae)
    Article Snippet: The PCR product was gel purified (TaKaRa Co., Dalian, China), ligated into T-vector (TaKaRa Co., Dalian, China), and the recombinant plasmid DNA was transformed into XL-1 blue competent bacteria. .. Positive clones were sequenced using the dideoxynucleotide chain termination method (TaKaRa Co., Dalian, China). ..

    Article Title: Potential Cooperations between Odorant-Binding Proteins of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)
    Article Snippet: .. Positive clones were selected for the sequence using the dideoxynucleotide chain termination method (TaKaRa Co., Dalian, China). ..

    Sequencing:

    Article Title: Potential Cooperations between Odorant-Binding Proteins of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)
    Article Snippet: .. Positive clones were selected for the sequence using the dideoxynucleotide chain termination method (TaKaRa Co., Dalian, China). ..



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