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fluorescent labeled dideoxynucleotide terminators  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fluorescent labeled dideoxynucleotide terminators
    Fluorescent Labeled Dideoxynucleotide Terminators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dideoxynucleotide+terminators/pmc03362512-160-6-20
    Average 90 stars, based on 1 article reviews
    fluorescent labeled dideoxynucleotide terminators - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Characterization of Newcastle Disease Viruses Isolated from Cormorant and Gull Species in the United States in 2010
    Article Snippet: PCR reactions were subjected to electrophoresis in 1% agarose gels and the DNA bands were excised from the gel and purified by using the QIAquick Gel Extraction Kit (Qiagen, Valencia, CA). .. All sequencing reactions were performed with fluorescent dideoxynucleotide terminators in the ABI 3700 automated sequencer (Applied Biosystems Inc., Foster City, CA). .. Sequence editing and assembly was performed with LaserGene sequence analysis software package (LaserGene, version 5.07; DNAStar, Inc., Madison, WI).

    Article Title: Phylogenetic Diversity among Low-Virulence Newcastle Disease Viruses from Waterfowl and Shorebirds and Comparison of Genotype Distributions to Those of Poultry-Origin Isolates
    Article Snippet: Amplified products were separated on a 1% agarose gel, the bands excised and eluted using the QIAquick Gel extraction kit (QIAGEN), and the samples quantified using a standard spectrophotometer. .. All sequencing reactions were performed with fluorescent dideoxynucleotide terminators in an automated sequencer (ABI 3700 automated sequencer; Applied Biosystems Inc., Foster City, CA). .. Nucleotide sequence editing and analyses were conducted with the LaserGene sequence analysis software package (LaserGene version 5.07; DNAStar, Inc., Madison, WI).

    Article Title: Molecular and ultrastructural confirmation of classification of ATCC 35122 as a strain of Pirellula staleyi.
    Article Snippet: .. A nearly complete 16S rRNA sequence was derived via cycle sequencing of the 16S rRNA gene (amplified by PCR using primers 27f and 1525r) employing ABI fluorescent dideoxynucleotide chain terminators and sequencing primers 342r, 687r, 945f, 1101r and 1114f, followed by electrophoresis on an ABI automated sequencer (Australian Genome Research Facility). ..

    Article Title: Protein Kinase Cε-dependent Regulation of Cystic Fibrosis Transmembrane Regulator Involves Binding to a Receptor for Activated C Kinase (RACK1) and RACK1 Binding to Na+/H+ Exchange Regulatory Factor
    Article Snippet: .. Automated sequencing reactions were performed with synthetic oligonucleotide primers and fluorescent dideoxynucleotide terminators on a DNA sequencer (model 377, Applied Biosystems, Inc., Foster City, CA). ..

    Article Title: Phylogenetic Diversity among Low-Virulence Newcastle Disease Viruses from Waterfowl and Shorebirds and Comparison of Genotype Distributions to Those of Poultry-Origin Isolates
    Article Snippet: Amplified products were separated on a 1% agarose gel, the bands excised and eluted using the QIAquick Gel extraction kit (QIAGEN), and the samples quantified using a standard spectrophotometer. .. All sequencing reactions were performed with fluorescent dideoxynucleotide terminators in an automated sequencer (ABI 3700 automated sequencer; Applied Biosystems Inc., Foster City, CA). .. Nucleotide sequence editing and analyses were conducted with the LaserGene sequence analysis software package (LaserGene version 5.07; DNAStar, Inc., Madison, WI).

    Article Title: Molecular Characterization of Podoviral Bacteriophages Virulent for Clostridium perfringens and Their Comparison with Members of the Picovirinae
    Article Snippet: Additionally, end-repair and G-tailing was completed for cloning restriction enzyme fragments into pSmart vectors (Lucigen™, Middleton, WI) for nucleotide sequencing. .. Double stranded nucleotide sequencing reactions using fluorescent labeled dideoxynucleotide terminators were also completed and sequences determined using an automated sequencer (Applied Biosystems Inc., Foster City, CA) . .. Nucleotide sequence assembly, editing, analysis, prediction of amino acid sequences and alignments were conducted using the MacVector 7.2TM (Accelrys, San Diego, CA) and DNASTARTM (Madison, WI) software.

    Derivative Assay:

    Article Title: Molecular and ultrastructural confirmation of classification of ATCC 35122 as a strain of Pirellula staleyi.
    Article Snippet: .. A nearly complete 16S rRNA sequence was derived via cycle sequencing of the 16S rRNA gene (amplified by PCR using primers 27f and 1525r) employing ABI fluorescent dideoxynucleotide chain terminators and sequencing primers 342r, 687r, 945f, 1101r and 1114f, followed by electrophoresis on an ABI automated sequencer (Australian Genome Research Facility). ..

    Amplification:

    Article Title: Molecular and ultrastructural confirmation of classification of ATCC 35122 as a strain of Pirellula staleyi.
    Article Snippet: .. A nearly complete 16S rRNA sequence was derived via cycle sequencing of the 16S rRNA gene (amplified by PCR using primers 27f and 1525r) employing ABI fluorescent dideoxynucleotide chain terminators and sequencing primers 342r, 687r, 945f, 1101r and 1114f, followed by electrophoresis on an ABI automated sequencer (Australian Genome Research Facility). ..

    Polymerase Chain Reaction:

    Article Title: Molecular and ultrastructural confirmation of classification of ATCC 35122 as a strain of Pirellula staleyi.
    Article Snippet: .. A nearly complete 16S rRNA sequence was derived via cycle sequencing of the 16S rRNA gene (amplified by PCR using primers 27f and 1525r) employing ABI fluorescent dideoxynucleotide chain terminators and sequencing primers 342r, 687r, 945f, 1101r and 1114f, followed by electrophoresis on an ABI automated sequencer (Australian Genome Research Facility). ..

    Article Title: Detection of the Emerging Picornavirus Senecavirus A in Pigs, Mice, and Houseflies
    Article Snippet: The PCR amplicons (F1 to F9) were subjected to electrophoresis in 1% agarose gels to confirm correct target amplification and were purified by using the GenJET PCR purification kit (Thermo Fisher Scientific, Waltham, MA). .. Purified PCR products were sequenced with fluorescent dideoxynucleotide terminators in an ABI 3700 automated sequencer (Applied Biosystems Inc., Foster City, CA). .. Sequence assembly and editing were performed with the Lasergene analysis software package (Lasergene version 5.07; DNAStar Inc., Madison, WI).

    Electrophoresis:

    Article Title: Molecular and ultrastructural confirmation of classification of ATCC 35122 as a strain of Pirellula staleyi.
    Article Snippet: .. A nearly complete 16S rRNA sequence was derived via cycle sequencing of the 16S rRNA gene (amplified by PCR using primers 27f and 1525r) employing ABI fluorescent dideoxynucleotide chain terminators and sequencing primers 342r, 687r, 945f, 1101r and 1114f, followed by electrophoresis on an ABI automated sequencer (Australian Genome Research Facility). ..

    Purification:

    Article Title: Detection of the Emerging Picornavirus Senecavirus A in Pigs, Mice, and Houseflies
    Article Snippet: The PCR amplicons (F1 to F9) were subjected to electrophoresis in 1% agarose gels to confirm correct target amplification and were purified by using the GenJET PCR purification kit (Thermo Fisher Scientific, Waltham, MA). .. Purified PCR products were sequenced with fluorescent dideoxynucleotide terminators in an ABI 3700 automated sequencer (Applied Biosystems Inc., Foster City, CA). .. Sequence assembly and editing were performed with the Lasergene analysis software package (Lasergene version 5.07; DNAStar Inc., Madison, WI).

    Labeling:

    Article Title: Molecular Characterization of Podoviral Bacteriophages Virulent for Clostridium perfringens and Their Comparison with Members of the Picovirinae
    Article Snippet: Additionally, end-repair and G-tailing was completed for cloning restriction enzyme fragments into pSmart vectors (Lucigen™, Middleton, WI) for nucleotide sequencing. .. Double stranded nucleotide sequencing reactions using fluorescent labeled dideoxynucleotide terminators were also completed and sequences determined using an automated sequencer (Applied Biosystems Inc., Foster City, CA) . .. Nucleotide sequence assembly, editing, analysis, prediction of amino acid sequences and alignments were conducted using the MacVector 7.2TM (Accelrys, San Diego, CA) and DNASTARTM (Madison, WI) software.



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