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Cosmo Bio USA polymorphprep
Polymorphprep, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+centrifugation+polymorphprep/polymorphprep/pm38937837-151-8-12
Average 90 stars, based on 1 article reviews
polymorphprep - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Isolation:

Article Title: Evidence of membranolytic targeting and intracellular citrullination in neutrophils isolated from patients with rheumatoid arthritis
Article Snippet: Alexa Fluor conjugated goat anti-mouse IgG Ab AF647 (Invitrogen, #A-21235), goat anti-mouse IgG (H + L) Ab AF488, (Invitrogen, #A-11001), goat anti-human IgG Ab AF555 (Invitrogen, #A21433), goat anti-Rabbit IgG Ab AF647 (Invitrogen, #A-21245). .. Neutrophils were isolated from venous blood samples by PolymorphPrep separation (CosmoBio, USA) according to the manufacturer's instruction. .. Neutrophils were cultured in DMEM high glucose (Thermo Fisher Scientific #11-965-118) on a glass-bottom 96-well plate (Cellvis, #P96-1-N) pre-coated with poly-L-lysine (Millipore-Sigma # P4707) for 30 min at 37 °C to allow the cells to adhere.

Article Title: Extensive citrullination of human serum albumin is physiological and not inherently immunogenic in rheumatoid arthritis.
Article Snippet: Horse radish peroxidase (HRP)-conjugated goat anti-human IgG was from ImmunoReagents, 3,3’,5,5’- tetramethylbenzidine (TMB) from BioLegend (#50-169-261), in-gel tryptic digestion kit Jo na l P re- pro of 16 from ThermoFisher Scientific (#89871X), EasyPep in-solution tryptic digestion kit from ThermoFisher Scientific (#A40006), 96% purified human serum albumin from ThermoFisher Scientific (#J66780.03), human AB Rh+ serum, heat-inactivated at 56°C, from Atlanta Biologicals (now R&D Systems) (#S40110H), recombinant PAD2 and PAD4 (Cayman), Pierce protein G agarose beads from ThermoFisher Scientific (#20397), AntiCitrulline (Modified) Detection Kit from Sigma-Aldrich (#17-346B-1), Thyroxine Competitive ELISA kit from Invitrogen/ThermoFisher Scientific (#EIATAC), and Immunoscan CCPlus from Svar Life Science AB (RA-96PLUS). .. Neutrophils were isolated from venous blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer's instructions. .. They were subsequently washed and suspended in DMEM high glucose (#11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes
Article Snippet: .. Neutrophils were isolated from freshly drawn blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer’s instructions. .. The cells were washed and suspended in DMEM high glucose (# 11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Evidence of membranolytic targeting and intracellular citrullination in neutrophils isolated from patients with rheumatoid arthritis.
Article Snippet: Alexa Fluor conjugated goat anti-mouse IgG Ab AF647 (Invitrogen, #A-21235), goat anti-mouse IgG (H + L) Ab AF488, (Invitrogen, #A-11001), goat anti-human IgG Ab AF555 (Invitrogen, #A21433), goat anti-Rabbit IgG Ab AF647 (Invitrogen, #A-21245). .. Neutrophil isolation from RA patients and healthy controls Neutrophils were isolated from venous blood samples by PolymorphPrep separation (CosmoBio, USA) according to the manufacturer’s instruction. .. Neutrophils were cultured in DMEM high glucose (Thermo Fisher Scientific #11-965-118) on a glass-bottom 96-well plate (Cellvis, #P96-1-N) pre-coated with poly-L-lysine (MilliporeSigma # P4707) for 30 min at 37 °C to allow the cells to adhere.

Article Title: Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes.
Article Snippet: .. Neutrophils were isolated from freshly drawn blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer’s instructions. .. The cells were washed and suspended in DMEM high glucose (# 11-965-118, Gibco, ThermoFisher Scientific).

Gradient Centrifugation:

Article Title: Extensive citrullination of human serum albumin is physiological and not inherently immunogenic in rheumatoid arthritis.
Article Snippet: Horse radish peroxidase (HRP)-conjugated goat anti-human IgG was from ImmunoReagents, 3,3’,5,5’- tetramethylbenzidine (TMB) from BioLegend (#50-169-261), in-gel tryptic digestion kit Jo na l P re- pro of 16 from ThermoFisher Scientific (#89871X), EasyPep in-solution tryptic digestion kit from ThermoFisher Scientific (#A40006), 96% purified human serum albumin from ThermoFisher Scientific (#J66780.03), human AB Rh+ serum, heat-inactivated at 56°C, from Atlanta Biologicals (now R&D Systems) (#S40110H), recombinant PAD2 and PAD4 (Cayman), Pierce protein G agarose beads from ThermoFisher Scientific (#20397), AntiCitrulline (Modified) Detection Kit from Sigma-Aldrich (#17-346B-1), Thyroxine Competitive ELISA kit from Invitrogen/ThermoFisher Scientific (#EIATAC), and Immunoscan CCPlus from Svar Life Science AB (RA-96PLUS). .. Neutrophils were isolated from venous blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer's instructions. .. They were subsequently washed and suspended in DMEM high glucose (#11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes
Article Snippet: .. Neutrophils were isolated from freshly drawn blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer’s instructions. .. The cells were washed and suspended in DMEM high glucose (# 11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes.
Article Snippet: .. Neutrophils were isolated from freshly drawn blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer’s instructions. .. The cells were washed and suspended in DMEM high glucose (# 11-965-118, Gibco, ThermoFisher Scientific).



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