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Progen Biotechnik density gradient centrifugation polymorphprep
Density Gradient Centrifugation Polymorphprep, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+centrifugation+polymorphprep/polymorphprep+gradient/pm35325781-70-38-42
Average 90 stars, based on 1 article reviews
density gradient centrifugation polymorphprep - by Bioz Stars, 2026-10
90/100 stars

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Isolation:

Article Title: Potential Pathogenetic Role of Antimicrobial Peptides Carried by Extracellular Vesicles in an in vitro Psoriatic Model
Article Snippet: .. Neutrophils were isolated from peripheral blood of healthy donors by centrifugation on a Polymorphprep (Progen, Heidelberg, Germany) density gradient. .. Isolated neutrophils were seeded at 3x10 4 /point on coverslips pretreated with poly-lysine and stimulated 3 hours with EVs obtained with HaCaT cells treated with inflammatory cytokines as previously described or with PMA (Sigma Aldrich, 50 ng/mL) as positive control.

Article Title: Bcl-xL targeting eliminates ageing tumor-promoting neutrophils and inhibits lung tumor growth.
Article Snippet: .. Neutrophils were isolated from the blood using a Polymorphprep (Progen, Cat# 1895) gradient and the remaining red blood cells were lysed with ACK-buffer. ..

Article Title: Deletion C-terminal thioesterase abolishes melanin biosynthesis, affects metabolism and reduces the pathogenesis of Fonsecaea monophora
Article Snippet: The samples were embedded in white resin, and thin sections were examined with a JEM-1400 PLUS Transmission Electron Microscope (Japan Electron Optics Laboratory Co., Ltd., Japan). .. Human neutrophils were isolated using PolymorphPrep density gradient media (1114683; PROGEN BioTEchnik GmbH, Heidelberg, Germany) according to the manufacturer’s protocol. ..

Article Title: Topical Application of the PI3Kβ-Selective Small Molecule Inhibitor TGX-221 Is an Effective Treatment Option for Experimental Epidermolysis Bullosa Acquisita
Article Snippet: .. For the PamGene analysis, FACS, NET formation, cytokine release, and ROS release assays, human PMNs were isolated from whole blood samples using a PolymorphPrepTM (Progen, Heidelberg, Germany) gradient according to the manufacturer's instructions. .. The purity of the PMNs was evaluated by flow cytometry (MACSQuant ® Analyzer 10, Miltenyi) using fluorescent staining with anti-human CD14 (clone HCD14, Biolegend) and anti-human CD15 antibodies (clone VIMC6, Miltenyi).

Article Title: TLR7 Activation in M-CSF-Dependent Monocyte-Derived Human Macrophages Potentiates Inflammatory Responses and Prompts Neutrophil Recruitment.
Article Snippet: Gene expression in breast carcinoma was assessed using the METABRIC (Molecular Taxonomy of Breast Cancer International Consortium) study cohort [55, 56] on the CBioportal (http://www.cbioportal.org). .. Neutrophil Isolation and Transmigration Assays Neutrophils were obtained from peripheral blood from anonymous healthy donors over a Polymorphprep (Progen) gradient according to manufacturer procedures. .. Neutrophil purity was assessed by flow cytometry (>95% CD15+ cells) (CD15 Monoclonal Antibody, APC, Invitrogen Cat. No. 17-0158-41).

Centrifugation:

Article Title: Potential Pathogenetic Role of Antimicrobial Peptides Carried by Extracellular Vesicles in an in vitro Psoriatic Model
Article Snippet: .. Neutrophils were isolated from peripheral blood of healthy donors by centrifugation on a Polymorphprep (Progen, Heidelberg, Germany) density gradient. .. Isolated neutrophils were seeded at 3x10 4 /point on coverslips pretreated with poly-lysine and stimulated 3 hours with EVs obtained with HaCaT cells treated with inflammatory cytokines as previously described or with PMA (Sigma Aldrich, 50 ng/mL) as positive control.

Clinical Proteomics:

Article Title: Serial measurement of M. tuberculosis in blood from critically-ill patients with HIV-associated tuberculosis
Article Snippet: .. For 10 randomly selected patient-timepoints, a 5 mL blood sample was separated into plasma, a buffy coat layer containing poly- and mono-nuclear white blood cells, and red cell pellet using density gradient centrifugation (PolymorphPrep, PROGEN, Heidelberg, Germany), then each sub-component was processed for Xpert-Ultra testing using the same protocol as for whole blood. ..

Article Title: Serial measurement of M. tuberculosis in blood from critically-ill patients with HIV-associated tuberculosis.
Article Snippet: .. Separation of blood components in subset of samples For 10 randomly selected patient-timepoints, a 5 mL blood sample was separated into plasma, a buffy coat layer containing poly- and mono-nuclear white blood cells, and red cell pellet using density gradient centrifugation (PolymorphPrep, PROGEN, Heidelberg, Germany), then each sub-component was processed for Xpert-Ultra testing using the same protocol as for whole blood. ..

Gradient Centrifugation:

Article Title: Serial measurement of M. tuberculosis in blood from critically-ill patients with HIV-associated tuberculosis
Article Snippet: .. For 10 randomly selected patient-timepoints, a 5 mL blood sample was separated into plasma, a buffy coat layer containing poly- and mono-nuclear white blood cells, and red cell pellet using density gradient centrifugation (PolymorphPrep, PROGEN, Heidelberg, Germany), then each sub-component was processed for Xpert-Ultra testing using the same protocol as for whole blood. ..

Article Title: Serial measurement of M. tuberculosis in blood from critically-ill patients with HIV-associated tuberculosis.
Article Snippet: .. Separation of blood components in subset of samples For 10 randomly selected patient-timepoints, a 5 mL blood sample was separated into plasma, a buffy coat layer containing poly- and mono-nuclear white blood cells, and red cell pellet using density gradient centrifugation (PolymorphPrep, PROGEN, Heidelberg, Germany), then each sub-component was processed for Xpert-Ultra testing using the same protocol as for whole blood. ..

FACS:

Article Title: Topical Application of the PI3Kβ-Selective Small Molecule Inhibitor TGX-221 Is an Effective Treatment Option for Experimental Epidermolysis Bullosa Acquisita
Article Snippet: .. For the PamGene analysis, FACS, NET formation, cytokine release, and ROS release assays, human PMNs were isolated from whole blood samples using a PolymorphPrepTM (Progen, Heidelberg, Germany) gradient according to the manufacturer's instructions. .. The purity of the PMNs was evaluated by flow cytometry (MACSQuant ® Analyzer 10, Miltenyi) using fluorescent staining with anti-human CD14 (clone HCD14, Biolegend) and anti-human CD15 antibodies (clone VIMC6, Miltenyi).

Transmigration Assay:

Article Title: TLR7 Activation in M-CSF-Dependent Monocyte-Derived Human Macrophages Potentiates Inflammatory Responses and Prompts Neutrophil Recruitment.
Article Snippet: Gene expression in breast carcinoma was assessed using the METABRIC (Molecular Taxonomy of Breast Cancer International Consortium) study cohort [55, 56] on the CBioportal (http://www.cbioportal.org). .. Neutrophil Isolation and Transmigration Assays Neutrophils were obtained from peripheral blood from anonymous healthy donors over a Polymorphprep (Progen) gradient according to manufacturer procedures. .. Neutrophil purity was assessed by flow cytometry (>95% CD15+ cells) (CD15 Monoclonal Antibody, APC, Invitrogen Cat. No. 17-0158-41).



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