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density gradient centrifugation polymorphprep  (Cosmo Bio USA)

 
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    Cosmo Bio USA density gradient centrifugation polymorphprep
    Density Gradient Centrifugation Polymorphprep, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/density+gradient+centrifugation+polymorphprep/polymorphprep+gradient+medium/pm32054060-72-21-25
    Average 90 stars, based on 1 article reviews
    density gradient centrifugation polymorphprep - by Bioz Stars, 2026-09
    90/100 stars

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    Isolation:

    Article Title: Pregnancy tailors endotoxin-induced monocyte and neutrophil responses in the maternal circulation.
    Article Snippet: .. Polymorphonuclear leukocytes (PMNs) were isolated using the Polymorphprep density gradient (Cosmo Bio USA, Inc., Carlsbad, CA, USA; cat# AXS1114683), per the manufacturer’s instructions. ..

    Article Title: Type I interferon-dependent IFIT3 signaling is critical for viral clearance in airway neutrophils
    Article Snippet: .. Neutrophils were isolated from whole blood using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol . ..

    Article Title: Interferon-dependent signaling is critical for viral clearance in airway neutrophils
    Article Snippet: Data were analyzed using the IDEAS software v6.1 (Luminex Corporation). .. In vitro transmigration Neutrophils were isolated from whole blood using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol (16). .. Neutrophil activation, purity (99% purity was obtained for every isolation), and viability was assessed using flow cytometry. and allowed to migrate into the chemoattractant LTB4 (100nM, Sigma) (A1 neutrophils, (16)) or into ALI/ARDS mBAL supernatant diluted 1:1 in plain RPMI (A2 neutrophils).

    Article Title: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.
    Article Snippet: .. In vitro transmigration Blood neutrophils were isolated using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol.48 Neutrophil purity and viability was assessed using flow cytometry. .. Purified neutrophils were loaded in the in vitro transmigration model18 as follows: 1) transmigration toward the neutrophil chemoattractant LTB4 (100nM, Sigma) (transmigrated neutrophils not undergoing GRIM adaptation); 2) transmigration using the CF airway supernatant (CFASN) generated from sputum as apical fluid, (transmigrated neutrophils undergoing GRIM adaptation); 3) transmigration using the COPD airway supernatant (COPDASN) generated from sputum as apical fluid; 4) CXCL-8 (Biolegend, 10 ng/mL); 5) LPS (Sigma, 10 ng/mL); and C5a (R&D systems, 500 ng/mL).

    Purification:

    Article Title: Pro-inflammatory cytokines associate with NETosis during sickle cell vaso-occlusive crises.
    Article Snippet: .. Neutrophils were purified from whole blood collected in EDTA from healthy donors using Polymorphprep gradient medium (Cosmo Bio USA, cat # AXS-1114683). ..

    Article Title: Pro-inflammatory cytokines associate with NETosis during sickle cell vaso-occlusive crises
    Article Snippet: .. Neutrophil extracellular traps (NETs) assay Neutrophils were purified from whole blood collected in EDTA from healthy donors using Polymorphprep gradient medium (Cosmo Bio USA, cat # AXS-1114683). ..

    In Vitro:

    Article Title: Interferon-dependent signaling is critical for viral clearance in airway neutrophils
    Article Snippet: Data were analyzed using the IDEAS software v6.1 (Luminex Corporation). .. In vitro transmigration Neutrophils were isolated from whole blood using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol (16). .. Neutrophil activation, purity (99% purity was obtained for every isolation), and viability was assessed using flow cytometry. and allowed to migrate into the chemoattractant LTB4 (100nM, Sigma) (A1 neutrophils, (16)) or into ALI/ARDS mBAL supernatant diluted 1:1 in plain RPMI (A2 neutrophils).

    Article Title: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.
    Article Snippet: .. In vitro transmigration Blood neutrophils were isolated using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol.48 Neutrophil purity and viability was assessed using flow cytometry. .. Purified neutrophils were loaded in the in vitro transmigration model18 as follows: 1) transmigration toward the neutrophil chemoattractant LTB4 (100nM, Sigma) (transmigrated neutrophils not undergoing GRIM adaptation); 2) transmigration using the CF airway supernatant (CFASN) generated from sputum as apical fluid, (transmigrated neutrophils undergoing GRIM adaptation); 3) transmigration using the COPD airway supernatant (COPDASN) generated from sputum as apical fluid; 4) CXCL-8 (Biolegend, 10 ng/mL); 5) LPS (Sigma, 10 ng/mL); and C5a (R&D systems, 500 ng/mL).

    Transmigration Assay:

    Article Title: Interferon-dependent signaling is critical for viral clearance in airway neutrophils
    Article Snippet: Data were analyzed using the IDEAS software v6.1 (Luminex Corporation). .. In vitro transmigration Neutrophils were isolated from whole blood using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol (16). .. Neutrophil activation, purity (99% purity was obtained for every isolation), and viability was assessed using flow cytometry. and allowed to migrate into the chemoattractant LTB4 (100nM, Sigma) (A1 neutrophils, (16)) or into ALI/ARDS mBAL supernatant diluted 1:1 in plain RPMI (A2 neutrophils).

    Article Title: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.
    Article Snippet: .. In vitro transmigration Blood neutrophils were isolated using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol.48 Neutrophil purity and viability was assessed using flow cytometry. .. Purified neutrophils were loaded in the in vitro transmigration model18 as follows: 1) transmigration toward the neutrophil chemoattractant LTB4 (100nM, Sigma) (transmigrated neutrophils not undergoing GRIM adaptation); 2) transmigration using the CF airway supernatant (CFASN) generated from sputum as apical fluid, (transmigrated neutrophils undergoing GRIM adaptation); 3) transmigration using the COPD airway supernatant (COPDASN) generated from sputum as apical fluid; 4) CXCL-8 (Biolegend, 10 ng/mL); 5) LPS (Sigma, 10 ng/mL); and C5a (R&D systems, 500 ng/mL).

    Flow Cytometry:

    Article Title: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.
    Article Snippet: .. In vitro transmigration Blood neutrophils were isolated using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol.48 Neutrophil purity and viability was assessed using flow cytometry. .. Purified neutrophils were loaded in the in vitro transmigration model18 as follows: 1) transmigration toward the neutrophil chemoattractant LTB4 (100nM, Sigma) (transmigrated neutrophils not undergoing GRIM adaptation); 2) transmigration using the CF airway supernatant (CFASN) generated from sputum as apical fluid, (transmigrated neutrophils undergoing GRIM adaptation); 3) transmigration using the COPD airway supernatant (COPDASN) generated from sputum as apical fluid; 4) CXCL-8 (Biolegend, 10 ng/mL); 5) LPS (Sigma, 10 ng/mL); and C5a (R&D systems, 500 ng/mL).



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