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Axis-Shield Diagnostics polymorphpreptm
Polymorphpreptm, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+centrifugation+polymorphprep/polymorphpreptm/pmc08997739-68-4-8
Average 90 stars, based on 1 article reviews
polymorphpreptm - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Gradient Centrifugation:

Article Title: Synthetic innate defense regulator peptide combination using CpG ODN as a novel adjuvant induces long‑lasting and balanced immune responses.
Article Snippet: .. Neutrophils were prepared by density gradient centrifugation using PolymorphprepTM (Axis-Shield Poc As, Oslo, Norway), according to the manufacturer's protocol. ..

Isolation:

Article Title: Yersinia pseudotuberculosis Blocks Neutrophil Degranulation
Article Snippet: .. 108 Isolation of human neutrophils 109 Neutrophils were isolated from heparinized blood (whole blood) from healthy donors using 110 PolymorphprepTM (Axis-Shield, Oslo, Norway), according to the manufacturer’s protocol. ..

Article Title: Neutrophil Extracellular Traps in Severe SARS-CoV-2 Infection: A Possible Impact of LPS and (1→3)-β-D-glucan in Blood from Gut Translocation
Article Snippet: .. Neutrophils were isolated by density centrifugation with PolymorphprepTM (Axis-Shield, Oslo, Norway) and were resuspended in RPMI 1640 supplemented with 10% fetal bovine serum. .. Then, purified healthy neutrophils (5 × 10 5 cells/well) were placed onto Poly-L- Lysine (Sigma-Aldrich, Singapore) coated on 6 mm glass coverslips and allowed to attach to the plate before challenging with 10% volume-by-volume ( v/v ) diluted serum from COVID-19 patients or healthy serum at 37 °C, 5% CO 2 incubator.

Article Title: Interleukin-8 and neutrophil extracellular traps in children with lupus nephritis and vitamin C deficiency.
Article Snippet: Background Vitamin C is a potent scavenger of reactive oxygen species, which induce neutrophil extracellular trap (NET) formation.. NETs are a major source of autoantigens and are involved in systemic lupus erythematosus (SLE) pathogenesis.. We determined vitamin C status and evaluated NET formation and inflammatory cytokines in children with lupus nephritis.

Article Title: β2 integrins (CD11/18) are essential for the chemosensory adhesion and migration of polymorphonuclear leukocytes on bacterial cellulose.
Article Snippet: Bacterial cellulose (BC) has been studied widely for applications in biomedical materials such as prosthetic artificial blood vessels owing to its unique characteristics, which include nontoxicity and nonimmunogenicity as compared with synthetic biopolymers such as expanded polytetrafluorethylene (ePTFE).. However, to date, studies on the relative effect of leukocytes on BC as a prosthetic vascular graft are insufficient.. Polymorphonuclear leukocytes (PMN) play a pivotal role in early-phase immune response to bacterial or periprosthetic infection.

Article Title: The infiltration and functional regulation of eosinophils induced by TSLP promote the proliferation of cervical cancer cell.
Article Snippet: Cervical cancer is often associated with eosinophil (EOS) infiltration, but the source and the role of EOS are still largely unknown.. Our previous work has established that thymic stromal lymphopoietin (TSLP) can stimulate the growth of cervical cancer cell in an autocrine manner.. Here, we report that EOS infiltration of the lesion site increased gradually with the progression of cervical cancer.

Article Title: Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection.
Article Snippet: .. Neutrophils were 612 isolated using PolymorphPrepTM (Axis-Shield). ..

Article Title: LL-37-mediated activation of host receptors is critical for defense against group A streptococcal infection.
Article Snippet: Blood was directly drawn into EDTA-coated tubes (BD Biosciences) by venipuncture of the forearm veins. .. PMNs were isolated using PolymorphprepTM (Axis-Shield, Norway) following the manufacturer’s protocol. ..

Centrifugation:

Article Title: Neutrophil Extracellular Traps in Severe SARS-CoV-2 Infection: A Possible Impact of LPS and (1→3)-β-D-glucan in Blood from Gut Translocation
Article Snippet: .. Neutrophils were isolated by density centrifugation with PolymorphprepTM (Axis-Shield, Oslo, Norway) and were resuspended in RPMI 1640 supplemented with 10% fetal bovine serum. .. Then, purified healthy neutrophils (5 × 10 5 cells/well) were placed onto Poly-L- Lysine (Sigma-Aldrich, Singapore) coated on 6 mm glass coverslips and allowed to attach to the plate before challenging with 10% volume-by-volume ( v/v ) diluted serum from COVID-19 patients or healthy serum at 37 °C, 5% CO 2 incubator.

Article Title: Interleukin-8 and neutrophil extracellular traps in children with lupus nephritis and vitamin C deficiency.
Article Snippet: Background Vitamin C is a potent scavenger of reactive oxygen species, which induce neutrophil extracellular trap (NET) formation.. NETs are a major source of autoantigens and are involved in systemic lupus erythematosus (SLE) pathogenesis.. We determined vitamin C status and evaluated NET formation and inflammatory cytokines in children with lupus nephritis.



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