other:Article Title: Subnuclear genome compartmentalization controls bivalent chromatin activity.
Article Snippet: For neuronal differentiation, NPCs were dissociated with Accutase and seeded at a density of 275,000 cells per cm2 on plates coated with 100 μg ml−1 poly-l-ornithine, 100 μg ml−1 poly-d-lysine, 10 μg ml−1 laminin and 10 μg ml−1 fibronectin (Sigma-Aldrich) in neuronal differentiation medium (Neurobasal-A supplemented with 2% B-27, 2 mM l-glutamine (Gibco), 200 μM l-ascorbic acid 2-phosphate, 10 μM dibutyryl-cyclic AMP (Sigma-Aldrich), 10 ng ml−1 BDNF (Peprotech), 10 ng ml−1 GDNF (Peprotech) and 10 μM DAPT (MedChemExpress)).
Article Title: An organoid model of the human adrenal cortex identifies drivers of steroidogenesis and zonation
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primocin Invivogen Cat #ant-pm-2 Matrigel Growth Factor Reduced Corning Cat #356231 Rspo3-Fc Fusion Protein conditioned media IpA Cat #R001 Noggin-Fc Fusion Protein conditioned media IpA Cat #N002 WNT Surrogate-FC Fusion Protein IpA Cat #N001 SB202190 Tocris Cat #1264 A83-01 Tocris Cat #2939 Prostaglandin E2 Tocris Cat #2296 Human FGF-basic (FGF-2/bFGF) ThermoFischer Scientific Cat #100-18B Y-27632 dihydrochloride Tocris Cat #1254 Forskolin Tocris Cat #1099 Angiotensin II R&D Systems Cat #1158 ACTH (1–39) Tocris Cat #3492 Human/Murine/Rat Activin A Recombinant Protein ThermoFischer Scientific Cat #120-14E Human BMP-2 Recombinant Protein ThermoFischer Scientific Cat #120-02 Human BMP-4 Recombinant Protein ThermoFischer Scientific Cat #120-05ET CHIR 99021 Tocris Cat #4423 Human EGF, Animal-Free Recombinant Protein ThermoFischer Scientific Cat #AF-100-15 GDF5 ThermoFischer Scientific Cat #120-01 Recombinant Human IGF-I Biolegend Cat #590908 Human IGF-II Recombinant Protein ThermoFischer Scientific Cat #100-12 Inhibin A R&D Systems Cat #8506-AB IWR-1 MedchemExpress Cat #HY-12238 IWP-2 MedchemExpress Cat #HY-13912 Recombinant Human Neuregulin-1 R&D Systems Cat #5898-NR Recombinant Human Neuregulin-3 R&D Systems Cat #11373-NR Human Sonic Hedgehog C25II Recombinant Protein ThermoFischer Scientific Cat #100-45 TGFβ1 R&D Systems Cat #7754-BH-005 Fludrocortisone acetate MedchemExpress Cat #HY-B1203A GW4064 Selleckchem Cat #S2782 FICZ Selleckchem Cat #S9682 GW3965 Selleckchem Cat #S2630 9-cis-retinoic acid MedchemExpress Cat #HY-15128 AHR antagonist 5 free base MedchemExpress Cat #HY-141609 Amodiaquine MedchemExpress Cat #HY-B1322B Clofibric acid MedchemExpress Cat #HY-B1415 Cytosporone B MedchemExpress Cat #HY-N2148 Gly-β-MCA MedchemExpress Cat #HY-114392 GW9662 MedchemExpress Cat #HY-16578 T0901317 MedchemExpress Cat #HY-10626 UVI 3003 MedchemExpress Cat #HY-107500 KCl ThermoFischer Scientific Cat #AM9640G PD 0325901 R&D Systems Cat #4192/10 Recombinant Human Norrin Protein, CF R&D Systems Cat #3014-NR DAPT MedchemExpress Cat #HY-13027 Vismodegib MedchemExpress Cat #HY-10440 Ketoconazole MedchemExpress Cat #HY-B0105 RIPA Lysis Buffer Thermo Fischer Scientific Cat #89901 Trypan Blue stain 0.4% Thermo Fischer Scientific Cat #T10282 (Continued on next page) Cell Press Blue 1, 100015, June 15, 2026 e2 Article ll OPEN ACCESS
Cell Culture:Article Title: CD36 Regulates PANoptosis in Diabetic Retinopathy via the NOTCH/MAML Pathway
Article Snippet: .. To modulate NOTCH signaling under HG conditions, cells were cultured in 25.0 mmol/L D‐glucose for 72 h. During the final 24 h, the HG group was supplemented with either 5 μ g/mL Jagged1 peptide (MedChemExpress, United States) to activate the pathway or 10 μ M DAPT (MedChemExpress, United States) to inhibit it. ..
Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
Recombinant:Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
Knock-Out:Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
Membrane:Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
Staining:Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
Filtration:Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
Flow Cytometry:Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
Fluorescence:Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
Microscopy:Article Title: Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth factor.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 BD Biosciences Cat#557508 Anti-CD34 BD Biosciences Cat#555824 (Continued on next page) 2 STAR Protocols 7, 104652, September 18, 2026 MATERIALS AND EQUIPMENT Continued REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ISL1 BD Biosciences Cat#562547 Anti-Ki67 BD Biosciences Cat#556026 Anti-TNNT2 Miltenyi Biotec Cat#MB-130-120-403 Anti-VE-cadherin BD Biosciences Cat#560410 Chemicals, peptides, and recombinant proteins 2-Mercaptoethanol Gibco Cat#21985-023 4% paraformaldehyde phosphate buffer solution Wako Cat#163-20145 Accutase Innovative Cell Technologies Cat#AT104 B-27 Supplement Minus Insulin (503) Gibco Cat#A1895601 B-27 Supplement (503) Gibco Cat#17504-044 BMP4 (human recombinant) PeproTech Cat#120-05ET CHIR98014 Sigma-Aldrich Cat#SML1094 CHIR99021 Sigma-Aldrich Cat#SML1046 DAPT MedChemExpress CatHY-13027 DMEM/F-12 Gibco Cat#11320-033 EGM-2 BulletKit Lonza Cat#CC-3162 Essential 8 medium Gibco Cat#A1517001 Fibronectin Sigma-Aldrich Cat#FC010 IWP2 Tocris Cat#3533 Knockout serum replacement Gibco Cat#10828010 L-Glutamine solution Wako Cat#073-05391 Matrigel Basement Membrane Matrix Corning Cat#354234 mTeSR1 medium STEMCELL Technologies Cat#ST-85850 N-2 Supplement (1003) Gibco Cat#17502-048 Neurobasal Medium Gibco Cat#21103-049 Penicillin/Streptomycin Gibco Cat#15140122 PLGF-2 (human recombinant) PeproTech Cat#100-56A RPMI 1640 (13) Gibco Cat#11875-093 SB431542 Tocris Cat#1614 StemPro-34 SFM Gibco Cat#10639-011 Trypan Blue Stain (0.4%) Gibco Cat#15250-061 VEGF-A (165) PeproTech Cat#100-20 Wnt-C59 Tocris Cat#5148 Y-27632 Tocris Cat#1254 ZM306416 Selleck Chemicals Cat#S2897 Experimental models: Cell lines Human ESC: H9 line WiCell Cat#WA09 Human ESC: HES-3 line WiCell Cat#ES03 Other Automated cell counter TC20 Bio-Rad Cat#1450102 Cell culture hood Thermo Scientific Cat#1300 SERIES A2 Centrifuge Thermo Scientific Cat#Sorvall X1 pro Filtration mesh Thermo Scientific Cat#566-0020 Flow cytometer BD Biosciences Cat#657427 Fluorescence microscope Nikon Cat#TE2000-EF-S Fluorescence-activated cell sorter Beckman Coulter Cat#AY42027 Incubator with both temperature and gas composition controls Astec Cat#SCA-165DRS Optical microscope with phase-contrast equipment Biotools Cat#MS-IV0205T Six-well culture plates Costar Cat#3506 Twelve-well culture plates Costar Cat#3512 Water bath AXEL Cat#TR-3a STAR Protocols 7, 104652, September 18, 2026 3 Note: Store at 4 ◦ C for up to 2 weeks. ..
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