Review



dapt solution  (Cayman Chemical)


Bioz Verified Symbol Cayman Chemical is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Cayman Chemical dapt solution
    Dapt Solution, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/dapt/pmc08355240-475-4-42
    Average 90 stars, based on 1 article reviews
    dapt solution - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Synaptic vesicle characterization of iPSC-derived dopaminergic neurons provides insight into distinct secretory vesicle pools.
    Article Snippet: Complete NB/B27 medium is comprised of N2 medium (without the N2 supplement) and the following components: 20 ng/ml BDNF (PeproTech), 0.2 mM ascorbic acid (SigmaAldrich), 20 ng/ml GDNF (PeproTech), 0.5mM db-cAMP (SigmaAldrich), 1 ng/ml TGFβ3 (R&D Systems) and 10 μM DAPT (Cayman Chemical Company).

    Article Title: Synaptic vesicle characterization of iPSC-derived dopaminergic neurons provides insight into distinct secretory vesicle pools
    Article Snippet: Complete NB/B27 medium is comprised of N2 medium (without the N2 supplement) and the following components: 20 ng/ml BDNF (PeproTech), 0.2 mM ascorbic acid (Sigma-Aldrich), 20 ng/ml GDNF (PeproTech), 0.5 mM db-cAMP (Sigma-Aldrich), 1 ng/ml TGFβ3 (R&D Systems) and 10 μM DAPT (Cayman Chemical Company).

    Article Title: Human Pulmonary Neuroendocrine Cells Respond to House Dust Mite Extract With PAR-1 Dependent Release of CGRP.
    Article Snippet: Once iPSCs were 80% confluent they were differentiated through definitive endoderm, anterior foregut endoderm, and lung- specific endoderm stages before they were matured to PNECs at ALI from day 17 onward using medium G (Figure S1B, Table S1) and 10 μM DAPT (Cayman Chemical) with media changes every other day [10]. nloaded from https://onlinelibrary.w iley.com /doi/10.1111/all.16416 by IN A SP - N E PA L , W iley O nline L ibrary on [01/12/2024].

    Article Title: Human Pulmonary Neuroendocrine Cells Respond to House Dust Mite Extract With PAR ‐1 Dependent Release of CGRP
    Article Snippet: Once HBEC were 80% confluent (1–3 days after seeding) they were matured to PNECs at ALI from day 17 onward using medium G (Table , [ ]) and 10 μM DAPT (Cayman Chemical) with media changes every other day [ ].

    Article Title: Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits
    Article Snippet: Post patterning Neural maturation medium was DMEM F12 medium containing N2, B27-VitA, 20 ng/mL GDNF (R&D Systems), 20 ng/mL BDNF (R&D Systems), 0.2 mM ascorbic acid (Sigma), 0.1 mM dibutyryl cAMP (Biolong), 10 μM DAPT (Cayman Chemical).

    Recombinant:


    Cell Culture:

    Article Title: Machine-learning system for diagnosing disorders and diseases and determining drug responsiveness
    Article Snippet: After 10 days incubation, aggregates were separated with accutase (Millipore, SCR0005) for 16-20 minutes at 37° C. with agitation, counted and plated on Matrigel coated plates density 50,000-60,000 cells/cm2. .. Cells were cultured the following 10 days (with medium changed every other day) in Differentiation Medium supplemented with 20 ng/ml BDNF (R&D, 248BD), 20 ng/ml GDNF (R&D, 212-GD), 10 μM DAPT (Cayman Chemicals, 13197), 0.2M L-Ascorbic Acid (Sigma, A4403), 1 ng/ml TGF3Beta (R&D, 243-B3-002) and 0.5 mM dbCAMP (Sigma, D0627). ..

    Article Title: hiPSC-neurons recapitulate the subtype-specific cell intrinsic nature of susceptibility to neurodegenerative disease-relevant aggregation.
    Article Snippet: On DIV-9, hiPSC-NPCs were harvested with Accutase and plated onto 77.5 μg/mL Matrigelcoated plates at a density of 2 million cells per well in N2B27 medium supplemented with 0.7 μM CHIR99021 (R&D Systems), 0.5 μM SAG (Sigma), 200 μM Ascorbic Acid (Sigma), and 10 μM Y-27632 (Millipore). .. Cells were subsequently cultured in N2B27 medium supplemented with 10 ng/mL BDNF, 10 ng/mL GDNF, 500 μM dcAMP, 1 ng/mL TGFβ3 (R&D Systems), 2.5 ng/mL Activin A, 200 μM Ascorbic Acid (Sigma), and 10 μM DAPT (Cayman Chemical). .. Neuronal differentiation was encouraged with the addition of the γ-secretase inhibitor RO4929097 (VWR) at 500 nM on DIV-5.



    Similar Products

    95
    MedChemExpress dapt solution
    Dapt Solution, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/DAPT/pm40341648-127-7-9
    Average 95 stars, based on 1 article reviews
    dapt solution - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    90
    Millipore γ-secretase inhibitor dapt solution
    γ Secretase Inhibitor Dapt Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/dapt/pmc10869796-352-3-7
    Average 90 stars, based on 1 article reviews
    γ-secretase inhibitor dapt solution - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Selleck Chemicals dmso working solution
    Dmso Working Solution, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/DAPT/pm37056149-49-20-25
    Average 96 stars, based on 1 article reviews
    dmso working solution - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    90
    FUJIFILM dapt solution
    The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor <t>DAPT.</t> (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without <t>50µM</t> of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.
    Dapt Solution, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/dapt/bio_rxiv__2023__07__19__549786-186-12-18
    Average 90 stars, based on 1 article reviews
    dapt solution - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Selleck Chemicals dapt working solution
    The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor <t>DAPT.</t> (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without <t>50µM</t> of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.
    Dapt Working Solution, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/DAPT/pm37083721-51-1-12
    Average 96 stars, based on 1 article reviews
    dapt working solution - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    95
    Thermo Fisher v v mem non essential amino acids solution thermo fisher scientific catalogue no 11140050
    The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor <t>DAPT.</t> (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without <t>50µM</t> of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.
    V V Mem Non Essential Amino Acids Solution Thermo Fisher Scientific Catalogue No 11140050, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/DAPT/pm36655405-292-27-33
    Average 95 stars, based on 1 article reviews
    v v mem non essential amino acids solution thermo fisher scientific catalogue no 11140050 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    90
    Merck & Co dapt stock solution
    The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor <t>DAPT.</t> (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without <t>50µM</t> of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.
    Dapt Stock Solution, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/dapt/pm35477753-482-0-5
    Average 90 stars, based on 1 article reviews
    dapt stock solution - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Cayman Chemical dapt solution
    The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor <t>DAPT.</t> (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without <t>50µM</t> of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.
    Dapt Solution, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/dapt/pmc08355240-475-4-42
    Average 90 stars, based on 1 article reviews
    dapt solution - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor DAPT. (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without 50µM of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.

    Journal: bioRxiv

    Article Title: The reciprocal regulation between mitochondrial-associated membranes and Notch signaling in skeletal muscle atrophy

    doi: 10.1101/2023.07.19.549786

    Figure Lengend Snippet: The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor DAPT. (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without 50µM of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.

    Article Snippet: TA muscle was removed 2 weeks after transplantation with several injections of DAPT solution (20µL of 50µM stock, WAKO) as shown in , fixed, and stained as above.

    Techniques:

    The regenerative capacity of Mfn2-deficient mouse muscle is reduced and that of Mfn2-deficient muscle stem cells when transplanted into Dystrophic muscle in vivo is improved by DAPT treatment of the muscle. (A) The flowchart to isolate muscle stem cells (MuSCs, SM-C/2.6+) and non-myogenic fibroblasts (FBs) derived from conditionally Mfn2 knockout mice after 4-OH tamoxifen (4-OHT) injection. (B) Immunostaining for Mfn2 (Green), Myogenin (Myog; Red), and DAPI (blue) on differentiated myotubes derived from wildtype or Mfn2-deficient mouse muscle stem cells sorted as SM-C/2.6 positive cells, co-cultured with non-myogenic fibroblasts (FBs). Scale bar; 50 µm. (C) Phase contrast images (left panels) and MAMs visualization (right panels) and quantitative analyses of MAM numbers (right) on cultured muscle stem cells. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) Western Blotting analyses of lysates from control and Mfn2-mutant cultured muscle stem cells. Nuclear lysates were analyzed with antibodies against NICD. Histone H3 was used as a loading control. (E) The flowchart for the transplantation into tibialis anterior (TA) muscles of DMD -/y mice (12 weeks old) with Mfn2-deficient muscle stem cells (1.0 x 10 4 cells) and the treatment with DAPT every 3∼4 days after the transplantation. (F) Transverse sectional images of TA muscles 14 days after the transplantation with the same number of muscle stem cells sorted as SM/C-2.6-positive cells derived from wildtype or conditional Mfn2 Knockout mice. Immunostaining for Dystrophin (Dmd, red as transplanted areas), laminin-a2 (Lama2, white to show the outline of myofibers), and DAPI (blue) on engrafted TA muscle after the transplantation. Scale bars; 50 µm. (G) The quantification of the total number of Dystrophin-positive (Dmd+) regenerated myofibers on the section transplanted with an equivalent number of normal or Mfn2-deficient MuSCs, with or without the treatment of the transplanted muscle with DAPT. (H) The average diameter of Dystrophin-positive (Dmd+) myofibers that are contributed by the transplanted MuSCs, as described in (G). All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests or one-way ANOVA and Tukey’s test for comparisons. *P<0.05.

    Journal: bioRxiv

    Article Title: The reciprocal regulation between mitochondrial-associated membranes and Notch signaling in skeletal muscle atrophy

    doi: 10.1101/2023.07.19.549786

    Figure Lengend Snippet: The regenerative capacity of Mfn2-deficient mouse muscle is reduced and that of Mfn2-deficient muscle stem cells when transplanted into Dystrophic muscle in vivo is improved by DAPT treatment of the muscle. (A) The flowchart to isolate muscle stem cells (MuSCs, SM-C/2.6+) and non-myogenic fibroblasts (FBs) derived from conditionally Mfn2 knockout mice after 4-OH tamoxifen (4-OHT) injection. (B) Immunostaining for Mfn2 (Green), Myogenin (Myog; Red), and DAPI (blue) on differentiated myotubes derived from wildtype or Mfn2-deficient mouse muscle stem cells sorted as SM-C/2.6 positive cells, co-cultured with non-myogenic fibroblasts (FBs). Scale bar; 50 µm. (C) Phase contrast images (left panels) and MAMs visualization (right panels) and quantitative analyses of MAM numbers (right) on cultured muscle stem cells. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) Western Blotting analyses of lysates from control and Mfn2-mutant cultured muscle stem cells. Nuclear lysates were analyzed with antibodies against NICD. Histone H3 was used as a loading control. (E) The flowchart for the transplantation into tibialis anterior (TA) muscles of DMD -/y mice (12 weeks old) with Mfn2-deficient muscle stem cells (1.0 x 10 4 cells) and the treatment with DAPT every 3∼4 days after the transplantation. (F) Transverse sectional images of TA muscles 14 days after the transplantation with the same number of muscle stem cells sorted as SM/C-2.6-positive cells derived from wildtype or conditional Mfn2 Knockout mice. Immunostaining for Dystrophin (Dmd, red as transplanted areas), laminin-a2 (Lama2, white to show the outline of myofibers), and DAPI (blue) on engrafted TA muscle after the transplantation. Scale bars; 50 µm. (G) The quantification of the total number of Dystrophin-positive (Dmd+) regenerated myofibers on the section transplanted with an equivalent number of normal or Mfn2-deficient MuSCs, with or without the treatment of the transplanted muscle with DAPT. (H) The average diameter of Dystrophin-positive (Dmd+) myofibers that are contributed by the transplanted MuSCs, as described in (G). All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests or one-way ANOVA and Tukey’s test for comparisons. *P<0.05.

    Article Snippet: TA muscle was removed 2 weeks after transplantation with several injections of DAPT solution (20µL of 50µM stock, WAKO) as shown in , fixed, and stained as above.

    Techniques: In Vivo, Derivative Assay, Knock-Out, Injection, Immunostaining, Cell Culture, Western Blot, Mutagenesis, Transplantation Assay