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γ-secretase inhibitor dapt solution  (Millipore)


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    Structured Review

    Millipore γ-secretase inhibitor dapt solution
    γ Secretase Inhibitor Dapt Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapt+solution/dapt/pmc10869796-352-3-7
    Average 90 stars, based on 1 article reviews
    γ-secretase inhibitor dapt solution - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Hepcidin expression is associated with increased γ-secretase–mediated cleavage of neogenin in the liver
    Article Snippet: The inhibitors used in the studies include α-secretase inhibitors, TAPI-1 (#B4686; APE × BIO) and Aderbasib (INCB007839; #HY-10293; MedChemExpress); γ-secretase inhibitors, DAPT (D5942; Sigma-Aldrich) and LY450139 (SML1938; Sigma-Aldrich); and proteasome inhibitor, MG132 (#47490; EMO Millipore Corp).

    Article Title: NOTCH1-STAT3 signaling axis regulates astrocytic differentiation of hippocampal neural stem/progenitor cells.
    Article Snippet: Hippocampal neural stem/progenitor cells (HNPCs) in the hippocampus can differentiate into neurons and astrocytes, and are regulated by complex signaling pathways, such as the NOTCH1–signal transducer and activator of transcription 3 (STAT3) axis, which are crucial for cell fate determination.. However, the exact molecular mechanism underlying HNPC differentiation remains unclear.. This study investigated the role of NOTCH1–STAT3 pathway in HNPC differentiation into neuronal and astrocytic lineages during embryonic development.

    Incubation:

    Article Title: WWC1 upregulation accelerates hyperuricemia by reduction in renal uric acid excretion through Hippo signaling pathway.
    Article Snippet: Received for publication, January 24, 2024, and in revised form, May 24, 2024 Published, Papers in Press, June 19, 2024, https://doi.org/10.1016/j.jbc.2024.107485 Changshun Han, Chengyong He, Xiaoyan Ding, Zixuan Li, Tianyun Peng, Chensong Zhang, Haibing Chen, Zhenghong Zuo, Jiyi Huang*, and Weiping Hu* From the Department of Nephrology, Fujian Clinical Research Center for Chronic Glomerular Disease, The First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China; Department of Endocrinology and Metabolism, Shanghai 10th People’s Hospital, Tongji University, Shanghai, China

    Staining:

    Article Title: WWC1 upregulation accelerates hyperuricemia by reduction in renal uric acid excretion through Hippo signaling pathway.
    Article Snippet: Received for publication, January 24, 2024, and in revised form, May 24, 2024 Published, Papers in Press, June 19, 2024, https://doi.org/10.1016/j.jbc.2024.107485 Changshun Han, Chengyong He, Xiaoyan Ding, Zixuan Li, Tianyun Peng, Chensong Zhang, Haibing Chen, Zhenghong Zuo, Jiyi Huang*, and Weiping Hu* From the Department of Nephrology, Fujian Clinical Research Center for Chronic Glomerular Disease, The First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China; Department of Endocrinology and Metabolism, Shanghai 10th People’s Hospital, Tongji University, Shanghai, China

    Cell Culture:

    Article Title: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling
    Article Snippet: Click-iT EdU Alexa Fluor 488 Imaging Kit (Fisher Scientific) instructions were followed and cells on coverslips were incubated with 10 μM EdU for 24 h before fixing. .. 500,000 hNSPCs were cultured in one well of a fibronectin-coated 12-well plate and treated with 10 μM DAPT (Calbiochem) or an equal volume of DMSO (Fisher Scientific) in proliferation media as a negative control for 24 h before dissociation and plating alone (100,000 hNSPCs for media control) or with hECs at a 1:1 ratio (48,000 hNSPCs:48,000 hECs) on 12 mm PDL/laminin coated coverslips. ..

    Article Title: PTPσ-mediated PI3P regulation modulates neurodegeneration in C9ORF72-ALS/FTD.
    Article Snippet: Article PTPs-mediated PI3P regu lation modulates neurodegeneration in C9ORF72-ALS/FTD

    Article Title: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling.
    Article Snippet: Click-iT EdUAlexa Fluor 488 ImagingKit (Fisher Scientific) instructions were followed and cells on coverslips were incubated with 10μM EdU for 24 h before fixing. .. Pre-DAPT treatment of hNSPCs for co-culture 500,000 hNSPCs were cultured in one well of a fibronectin-coated 12- well plate and treated with 10μM DAPT (Calbiochem) or an equal volumeof DMSO (Fisher Scientific) in proliferationmedia as a negative control for 24 h before dissociation and plating alone (100,000 hNSPCs for media control) or with hECs at a 1:1 ratio (48,000 hNSPCs:48,000 hECs) on 12mm PDL/laminin coated coverslips. ..

    Negative Control:

    Article Title: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling
    Article Snippet: Click-iT EdU Alexa Fluor 488 Imaging Kit (Fisher Scientific) instructions were followed and cells on coverslips were incubated with 10 μM EdU for 24 h before fixing. .. 500,000 hNSPCs were cultured in one well of a fibronectin-coated 12-well plate and treated with 10 μM DAPT (Calbiochem) or an equal volume of DMSO (Fisher Scientific) in proliferation media as a negative control for 24 h before dissociation and plating alone (100,000 hNSPCs for media control) or with hECs at a 1:1 ratio (48,000 hNSPCs:48,000 hECs) on 12 mm PDL/laminin coated coverslips. ..

    Article Title: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling.
    Article Snippet: Click-iT EdUAlexa Fluor 488 ImagingKit (Fisher Scientific) instructions were followed and cells on coverslips were incubated with 10μM EdU for 24 h before fixing. .. Pre-DAPT treatment of hNSPCs for co-culture 500,000 hNSPCs were cultured in one well of a fibronectin-coated 12- well plate and treated with 10μM DAPT (Calbiochem) or an equal volumeof DMSO (Fisher Scientific) in proliferationmedia as a negative control for 24 h before dissociation and plating alone (100,000 hNSPCs for media control) or with hECs at a 1:1 ratio (48,000 hNSPCs:48,000 hECs) on 12mm PDL/laminin coated coverslips. ..

    Control:

    Article Title: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling
    Article Snippet: Click-iT EdU Alexa Fluor 488 Imaging Kit (Fisher Scientific) instructions were followed and cells on coverslips were incubated with 10 μM EdU for 24 h before fixing. .. 500,000 hNSPCs were cultured in one well of a fibronectin-coated 12-well plate and treated with 10 μM DAPT (Calbiochem) or an equal volume of DMSO (Fisher Scientific) in proliferation media as a negative control for 24 h before dissociation and plating alone (100,000 hNSPCs for media control) or with hECs at a 1:1 ratio (48,000 hNSPCs:48,000 hECs) on 12 mm PDL/laminin coated coverslips. ..

    Article Title: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling.
    Article Snippet: Click-iT EdUAlexa Fluor 488 ImagingKit (Fisher Scientific) instructions were followed and cells on coverslips were incubated with 10μM EdU for 24 h before fixing. .. Pre-DAPT treatment of hNSPCs for co-culture 500,000 hNSPCs were cultured in one well of a fibronectin-coated 12- well plate and treated with 10μM DAPT (Calbiochem) or an equal volumeof DMSO (Fisher Scientific) in proliferationmedia as a negative control for 24 h before dissociation and plating alone (100,000 hNSPCs for media control) or with hECs at a 1:1 ratio (48,000 hNSPCs:48,000 hECs) on 12mm PDL/laminin coated coverslips. ..



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    The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor DAPT. (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without 50µM of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.

    Journal: bioRxiv

    Article Title: The reciprocal regulation between mitochondrial-associated membranes and Notch signaling in skeletal muscle atrophy

    doi: 10.1101/2023.07.19.549786

    Figure Lengend Snippet: The improvement of mitochondrial abnormalities in MFN2-deficient human iPS cells treated with gamma-secretase inhibitor DAPT. (A) Mitochondrial morphology visualized by MitoTracker in MFN2-deficient human iPS cells with or without 50µM of DAPT. (Right panels; magnified area outlined in right panels), Scale bars; 50 µm. (B) Relative transcription levels of HES1 and HEY1 in MFN2-deficient human iPS cells with or without DAPT. (C) MAM visualization in MFN2-deficient human iPS cells, with or without DAPT. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) The quantitative analyses of MAM numbers in MFN2-deficient human iPS cells with or without DAPT. (E) Total ATP production in MFN2-deficient human iPS cells treated with or without DAPT. All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests for comparisons. *P<0.05.

    Article Snippet: TA muscle was removed 2 weeks after transplantation with several injections of DAPT solution (20µL of 50µM stock, WAKO) as shown in , fixed, and stained as above.

    Techniques:

    The regenerative capacity of Mfn2-deficient mouse muscle is reduced and that of Mfn2-deficient muscle stem cells when transplanted into Dystrophic muscle in vivo is improved by DAPT treatment of the muscle. (A) The flowchart to isolate muscle stem cells (MuSCs, SM-C/2.6+) and non-myogenic fibroblasts (FBs) derived from conditionally Mfn2 knockout mice after 4-OH tamoxifen (4-OHT) injection. (B) Immunostaining for Mfn2 (Green), Myogenin (Myog; Red), and DAPI (blue) on differentiated myotubes derived from wildtype or Mfn2-deficient mouse muscle stem cells sorted as SM-C/2.6 positive cells, co-cultured with non-myogenic fibroblasts (FBs). Scale bar; 50 µm. (C) Phase contrast images (left panels) and MAMs visualization (right panels) and quantitative analyses of MAM numbers (right) on cultured muscle stem cells. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) Western Blotting analyses of lysates from control and Mfn2-mutant cultured muscle stem cells. Nuclear lysates were analyzed with antibodies against NICD. Histone H3 was used as a loading control. (E) The flowchart for the transplantation into tibialis anterior (TA) muscles of DMD -/y mice (12 weeks old) with Mfn2-deficient muscle stem cells (1.0 x 10 4 cells) and the treatment with DAPT every 3∼4 days after the transplantation. (F) Transverse sectional images of TA muscles 14 days after the transplantation with the same number of muscle stem cells sorted as SM/C-2.6-positive cells derived from wildtype or conditional Mfn2 Knockout mice. Immunostaining for Dystrophin (Dmd, red as transplanted areas), laminin-a2 (Lama2, white to show the outline of myofibers), and DAPI (blue) on engrafted TA muscle after the transplantation. Scale bars; 50 µm. (G) The quantification of the total number of Dystrophin-positive (Dmd+) regenerated myofibers on the section transplanted with an equivalent number of normal or Mfn2-deficient MuSCs, with or without the treatment of the transplanted muscle with DAPT. (H) The average diameter of Dystrophin-positive (Dmd+) myofibers that are contributed by the transplanted MuSCs, as described in (G). All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests or one-way ANOVA and Tukey’s test for comparisons. *P<0.05.

    Journal: bioRxiv

    Article Title: The reciprocal regulation between mitochondrial-associated membranes and Notch signaling in skeletal muscle atrophy

    doi: 10.1101/2023.07.19.549786

    Figure Lengend Snippet: The regenerative capacity of Mfn2-deficient mouse muscle is reduced and that of Mfn2-deficient muscle stem cells when transplanted into Dystrophic muscle in vivo is improved by DAPT treatment of the muscle. (A) The flowchart to isolate muscle stem cells (MuSCs, SM-C/2.6+) and non-myogenic fibroblasts (FBs) derived from conditionally Mfn2 knockout mice after 4-OH tamoxifen (4-OHT) injection. (B) Immunostaining for Mfn2 (Green), Myogenin (Myog; Red), and DAPI (blue) on differentiated myotubes derived from wildtype or Mfn2-deficient mouse muscle stem cells sorted as SM-C/2.6 positive cells, co-cultured with non-myogenic fibroblasts (FBs). Scale bar; 50 µm. (C) Phase contrast images (left panels) and MAMs visualization (right panels) and quantitative analyses of MAM numbers (right) on cultured muscle stem cells. (Red; MAM (IP3R-VDAC1 PLA), blue; DAPI, Scale bars; 20 µm. (D) Western Blotting analyses of lysates from control and Mfn2-mutant cultured muscle stem cells. Nuclear lysates were analyzed with antibodies against NICD. Histone H3 was used as a loading control. (E) The flowchart for the transplantation into tibialis anterior (TA) muscles of DMD -/y mice (12 weeks old) with Mfn2-deficient muscle stem cells (1.0 x 10 4 cells) and the treatment with DAPT every 3∼4 days after the transplantation. (F) Transverse sectional images of TA muscles 14 days after the transplantation with the same number of muscle stem cells sorted as SM/C-2.6-positive cells derived from wildtype or conditional Mfn2 Knockout mice. Immunostaining for Dystrophin (Dmd, red as transplanted areas), laminin-a2 (Lama2, white to show the outline of myofibers), and DAPI (blue) on engrafted TA muscle after the transplantation. Scale bars; 50 µm. (G) The quantification of the total number of Dystrophin-positive (Dmd+) regenerated myofibers on the section transplanted with an equivalent number of normal or Mfn2-deficient MuSCs, with or without the treatment of the transplanted muscle with DAPT. (H) The average diameter of Dystrophin-positive (Dmd+) myofibers that are contributed by the transplanted MuSCs, as described in (G). All error bars indicate ±SEM (n=5). P -values are determined by non-parametric Wilcoxon tests or one-way ANOVA and Tukey’s test for comparisons. *P<0.05.

    Article Snippet: TA muscle was removed 2 weeks after transplantation with several injections of DAPT solution (20µL of 50µM stock, WAKO) as shown in , fixed, and stained as above.

    Techniques: In Vivo, Derivative Assay, Knock-Out, Injection, Immunostaining, Cell Culture, Western Blot, Mutagenesis, Transplantation Assay