v v mem non essential amino acids solution thermo fisher scientific catalogue no 11140050 (Thermo Fisher)

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Thermo Fisher v v mem non essential amino acids solution thermo fisher scientific catalogue no 11140050
V V Mem Non Essential Amino Acids Solution Thermo Fisher Scientific Catalogue No 11140050, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapt+solution/DAPT/pm36655405-292-27-33
Average 95 stars, based on 1 article reviews

v v mem non essential amino acids solution thermo fisher scientific catalogue no 11140050 - by Bioz Stars, 2026-09

95/100 stars

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Recombinant:

Article Title: Blocking apoptosis promotes survival and alters developmental dynamics of human retinal ganglion cells in retinal organoids.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins mTeSRTM1 Stem Cell Technologies Cat #85857 Matrigel-GFRTM BD Biosciences Cat #354230 Accutase Sigma Cat #SCR005 Blebbistatin Sigma Cat #B0560 Insulin Roche Cat #11376497001 holo-transferrin Sigma Cat #T0665 L-ascorbic acid Sigma Cat #A8960 sodium selenite Sigma Cat #S5261 B27 supplement minus Vitamin A (50X) Thermo Fisher Scientific Cat #12587010 Glutamax Thermo Fisher Scientific Cat #35050061 NEAA Thermo Fisher Scientific Cat #11140050 Sodium Pyruvate Thermo Fisher Scientific Cat #11360070 F12 Thermo Fisher Scientific Cat #11765062 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 heat-inactivated FBS Thermo Fisher Scientific Cat #16140071 taurine Sigma Cat #T-8691 NaCl Sigma Cat #S3014 DMEM Thermo Fisher Scientific Cat #11885084 all-trans retinoic acid (ATRA) Sigma Cat #R2625 Gamma-secretase inhibitor (DAPT) EMD Millipore Cat #565770 Formaldehyde (16%), Methanol-free Thermo Fisher Scientific Cat #28906 PBS Thermo Fisher Scientific Cat #AM9624 O.C.T Sakura Finetek Cat #12351753 Donkey serum Millipore Cat #S30-M Triton X-100 Sigma Cat #T9284 DAPI Thermo Fisher Scientific Cat #62248 Hoechst Biotium Cat #40046 SlowFade Gold Antifade mountant Thermo Fisher Scientific Cat #P36940 Propidium iodide Sigma-Aldrich Cat #P4170 Critical commercial assays MycoAlert Lonza Cat #LT07 Click-iTTM Plus TUNEL Assay Invitrogen Cat #C10617 Click-iT EdU Assay Invitrogen Cat #C10640 Deposited data 10x Chromium snRNA-seq data This paper GSE305194 Original code for snRNA-seq analysis This paper https://github.com/BGuy2/SZ_BAX-BAKdKO/ Experimental models: Cell lines H7 POU4F2-P2A-tdTomato-P2AmTHY1.2 hESC line Prof. Donald Zack, JHMI Sluch et al.58 H9 POU4F2-P2A-mNeon-H2B hESC line Prof. Karl Wahlin, UCSD Agarwal et al.64 IMR90.4 SIX6-P2A-H2B-GFP hiPSC line Prof. Donald Zack, JHMI Wahlin et al.79 GM25256 iPSC line Prof. Vivian Gama, Vanderbilt University Joshi et al.87 GM25256 BAX/BAK double knockout iPSC line Prof. Vivian Gama, Vanderbilt University Joshi et al.87 Software and algorithms Fiji ImageJ https://imagej.net/software/fiji/ Prism v10.4.1 GraphPad software https://www.graphpad.com/ (Continued on next page) 20 Cell Reports 45, 117270, April 28, 2026 ..

Article Title: Small molecule-directed differentiation of submerged-cultured human nasal airway epithelia for respiratory disease modeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-beta IV Tubulin antibody [EPR16776] Abcam Cat#ab179509; RRID: AB_2716759 Anti-Beta-Tubulin IV [ONS1A6] Emergo Biogenex Cat#MU178-UC; RRID: AB_2335625 MUC5AC Monoclonal Antibody (45M) Thermo Fisher Scientific Cat#MA1-38223; RRID: AB_2266697 Anti-p63 antibody [EPR5701] Abcam Cat#ab124762; RRID: AB_10971840 FOXJ1 Monoclonal Antibody (2A5) Thermo Fisher Scientific Cat#14-9965-80; RRID: AB_1548836 SLPI, Human, pAb Hycult Biotech Cat#HP9024; RRID: AB_2286624 Human pIgR Antibody R&D systems Cat#MAB27171; RRID: AB_3657978 Monoclonal Antibody to CC16 Origene Tech Cat#AM26360PU-N; RRID: AB_11216041 Anti-RSV Antibody, clone 133-1H Merck Cat#MAB8262; RRID: AB_95302 Anti-Rabbit IgG, Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11034; RRID: AB_2576217 Anti-Mouse IgG1, Alexa Fluor 647 Thermo Fisher Scientific Cat#A-21240; RRID: AB_2535809 Chemicals and recombinant proteins DMEM Thermo Fisher Scientific Cat#12634-028 Opti-MEM Thermo Fisher Scientific Cat#31985062 BEpiCM-b Sciencell Cat#3211 advanced DMEM/F-12 Thermo Fisher Scientific Cat#12634-028 B-27 Supplement, serum free Thermo Fisher Scientific Cat#17504001 HEPES Thermo Fisher Scientific Cat#15630080 GlutaMAX Thermo Fisher Scientific Cat#35050-061 Hydrocortisone Sigma-Aldrich Cat#H0888 Epinephrine hydrochloride Sigma-Aldrich Cat#E4642 N-acetyl-L-cysteine Sigma-Aldrich Cat#A9165 Nicotinamide Sigma-Aldrich Cat#N0636 3,3′,5-Triiodo-L-thyronine sodium salt Sigma-Aldrich Cat#T6397 Penicillin/streptomycin Thermo Fisher Scientific Cat#15070-063 Primocin InvivoGen Cat#ant-pm-2 Amphotericin B Thermo Fisher Scientific Cat#15290018 Gentamicin Sigma-Aldrich Cat#G1397 Vancomycin Sigma-Aldrich Cat#SBR00001 A83-01 Tocris Cat#2939/10 Y-27632 Selleck Chemicals Cat#S1049 Rapamycin Sigma-Aldrich Cat#553210 DAPT Thermo Fisher Scientific Cat15467109 DMH1 Selleck Chemicals Cat#S7146 TTNPB Cayman Cat#16144-1 RSPO3-Fc Fusion Protein CM U-Protein Express Cat#R001 Recombinant human Heregulin-β1 PeproTech Cat#100-03 Recombinant human FGF10 PeproTech Cat#100-26 Recombinant human HGF PeproTech Cat#100-39H Recombinant human EGF PeproTech Cat#AF-100-15 Recombinant human FGF7 PeproTech Cat#100-19 Recombinant Interleukin-1β PeproTech Cat#200-01 Nirsevimab Provided by AstraZeneca N/A (Continued on next page) e1 Cell Reports Medicine 7, 102692, April 21, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagen IV Sigma-Aldrich Cat#C7521 PureCol Advanced BioMatrix Cat#5005 Cultrex Basement Membrane Extract, Type 2 Trevigen Cat#3532-010 TrypLE express enzyme Thermo Fisher Scientific Cat#12605010 CryoStor CS10 STEMCELL Technologies Cat#07930 SiR-tubulin Spirochrome AG Cat#SC002 DAPI Sigma-Aldrich Cat#D9542 ProLong Gold Thermo Fisher Scientific Cat#P36934 VX-809 Selleck Chemicals Cat#S1565 VX-661 Selleck Chemicals Cat#S7059 VX-445 MedChemExpress Cat#HY-11177 VX-770 Selleck Chemicals Cat#S1144 Calcein green (AM) Invitrogen Cat #C34852 Forskolin Sigma-Aldrich Cat#F3917 G418 (Geneticin) Invivogen Cat#GNL-40-03 ELX-02 (Exaluren) MedChemExpress Cat#HY-114231 Cultureware 96-well culture plates Greiner Bio-One Cat# 655182 24-well culture plates Greiner Bio-One Cat# 662160 12-well culture plates Greiner Bio-One Cat#665165 6-well culture plates Greiner Bio-One Cat#657160 6.5 mm Transwell with 0.4 μm Pore Polyester Membrane Insert Corning Cat#3470 Nunc dishes (35 mm) with UpCell Surface Thermo Fisher Scientific Cat#174904 Commercial kits RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA synthesis kit Bio-Rad Cat#1708891 iQ SYBR Green Supermix Bio-Rad Cat#1708880 Oligonucleotides qPCR Primers This papers, Table S4 N/A Experimental models: Primary cells and cell lines Human Nasal Epithelial Cells (HNEC) UMC Utrecht Protocol ID: 16–586, and 21-044 Human Epithelioma-2 (HEp-2) ATCC Cat#CCL-23 Experimental models: viral strains mKate-RSV-A2 Hotard et al.37 N/A RSV-A and -B clinical isolate strains Langedijk et al.38 N/A Equipment Thunder Imager 3D live Cell with Leica N/A Leica DFC9000 GCT camera Leica N/A Zeiss LSM800 confocal microscope Zeiss N/A Nanodrop spectrophotometer Thermo Fisher N/A CFX96 real-time detection machine Bio-Rad N/A BD FACSJazz BD Bioscience N/A Software and algorithms Leica LAS X Software Leica https://www.leicamicrosystems.com/ Zen Blue Software Zeiss https://www.zeiss.com/ ImageJ/FIJI NIH, Fiji developers https://imagej.net/Fiji/ GNU Image Manipulation Program https://www.gimp.org/ (Continued on next page) Cell Reports Medicine 7, 102692, April 21, 2026 e2

TUNEL Assay:

EdU Assay:

Double Knockout:

Software:

Extraction:

Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
Article Snippet: .. After confirming the involvement of MT1MMP, the above cultures were also treated with or without Marimastat (10 μM) (MMP inhibitor) or DAPT (10 μM) (γ-secretase inhibitor) or both for 0 and 5 min for membranous, cytoplasmic and nuclear preparation using NEPER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher Scientific, Waltham, MA) before Western blot of nuclear pSMAD2/3, CD44ICD, active MT1MMP and active γ-secretase, and for 0, 5, 15, 30 and 45 min for immunostaining of CD44ICD, cyclin D1 and p75NTR. .. Total RNAs were extracted using RNeasy Mini Kit and reverse- transcribed using High-Capacity Reverse Transcription Kit. cDNA was amplified by real-time RT-PCR using specific primers and PCR Master Mix in ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Quant Studio 5 Real-time PCR System (ThermoFisher Scientific, Waltham, MA).

Western Blot:

Immunostaining:

Cell Isolation:

Article Title: Immortalization of human airway epithelial cells for genome editing applications
Article Snippet: Please see licensing information on first page for reuse rights. https://publications.ersnet.orgDownloaded from Glutamax 1 x 5 mL Thermo Fisher Scientific Penicilin/Streptomyc in (Pen/Strep) 1 x 5 mL Thermo Fisher Scientific Nasal cell basic medium Bronchial Epithelial Cell Medium (BEPICM) 50% ScienCell Advanced DMEM F12 44% Gibco B27 2% Thermo Fisher Scientific HEPES 1 M 10 mM Thermo Fisher Scientific Glutamax 1 x 5 mL Thermo Fisher Scientific Pen/Strep 1 x 5 mL Thermo Fisher Scientific Hydrocortisone 0,5 μg/ml Sigma-Aldrich N-acetyl-cysteine 1,25 mM Sigma-Aldrich Primocin 100 μg/ml InvivoGen A83-01 1 μM Tocris #2939/10 Epinephrine 0,5 μg/ml Sigma-Aldrich Y-27632 5 μM Selleck Chemicals #S1049 Nasal cell isolation medium Nasal cell basic medium R-spondin3 (RSPO3) 2% U-Protein Express Heregulin beta-1 50 nM PeproTech #100-03 Fibroblast Growth Factor (FGF) 10 100 ng/ml PeproTech #100-26 Hepatocyte Growth Factor (HGF) 25 ng/ml PeproTech #100-39 Amphotericin B 250 μg/ml Thermo Fisher Scientific Gentamycin 50 μg/ml Sigma-Aldrich Vancomycin 50 μg/ml Sigma-Aldrich Nasal cell expansion medium Nasal cell basic medium RSPO3 2% U-Protein Express on June 27, 2026 by guest. .. Please see licensing information on first page for reuse rights. https://publications.ersnet.orgDownloaded from Heregulin beta-1 5 nM PeproTech #100-03 DAPT 5 μM Thermo Fisher Scientific #15467109 FGF10 100 ng/ml PeproTech #100-26 HGF 25 ng/ml PeproTech #100-39 Rapamycin 50 nM Thermo Fisher Scientific Bronchial cell basic medium BEPICM 50 % ScienCell Advanced DMEM F12- 43,4 % Gibco HEPES 10 mM Thermo Fisher Scientific Glutamax 1 x Thermo Fisher Scientific Pen/Strep 1 x Thermo Fisher Scientific Primocin 100 μg/ml InvivoGen B27 2 % Thermo Fisher Scientific Hydrocortisone 0,5 μg/ml Sigma-Aldrich Triiodothyronine, T3 100 nM Sigma-Aldrich Epinephrine 0,5 μg/ml Sigma-Aldrich N-acetyl-cysteine 1,25 mM Sigma-Aldrich Nicotinamide 5 mM Sigma-Aldrich A83-01 1 μM Tocris #2939/10 DMH-1 1 μM Selleck Chemicals # S7146 Y-27632 5 μM Selleck Chemicals SB 202190 500 nM Sigma-Aldrich #S7067 Bronchial cell isolation medium Bronchial cell basic medium FGF10 100 ng/ml PeproTech #100-26 FGF7 25 ng/ml PeproTech #100-19 HGF 25 ng/ml PeproTech #100-39 Human Epidermal Growth Factor (hEGF) 5 ng/ml) PeproTech #100-15 Amphotericin B 250 μg/ml Thermo Fisher Scientific on June 27, 2026 by guest. .. Please see licensing information on first page for reuse rights. https://publications.ersnet.orgDownloaded from Gentamycin 50 μg/ml Sigma-Aldrich Vancomycin 50 μg/ml Sigma-Aldrich Bronchial cell expansion medium Bronchial cell basic medium RSPO3 2% U-Protein Express FGF10 (100 ng/ml) PeproTech #100-26 FGF7 (25 ng/ml) PeproTech #100-19 HGF (25 ng/ml) PeproTech #100-39 hEGF (5 ng/ml) PeproTech #100-15 DAPT 5 μg/ml Thermo Fisher Scientific #15467109 ALI-differentiation medium Advanced DMEM/F12 Gibco A83-01 50 nM Tocris #2939/10 hEGF 0.5 ng/ml PeproTech #100-15 Triiodothyronine, T3 100 nM Sigma-Aldrich Epinephrine 0.5 μg/ml Sigma-Aldrich TTNPB 100 nM Cayman Hydrocortisone 0.5 μg/ml Sigma-Aldrich Pen/Strep 1 % v/v Sigma-Aldrich ALI-differentiation medium supplements during air-exposure A83-01 500 nM Tocris #2939/10 DAPT 20 μM Thermo Fisher Scientific #15467109 DMH-1 5 μM Selleck Chemicals #S7146 Software and algorithms ICE analysis Ref. [10] on June 27, 2026 by guest.

other:

Article Title: A human neuron-microglia tri-culture platform to study the influence of microglia on developing neuronal networks in vitro
Article Snippet: On day 2, a full media change was performed with CompGN+D with DAPT (Thermo Scientific Chemicals, J65864.MA).

Concentration Assay:

Article Title: Differentiation method
Article Snippet: .. Improved differentiation medium (DM+) Concentration Component Gibco Advanced DMEM/F12 10 mM HEPES 1 x B27 wo VitA 1 x N2 1.25 mM n-Acetylcysteine 50 ng/ml hEGF 10 nM Gastrin 25 ng/ml HGF 0.5 uM A83.01 25 ng/ml BMP7 100 ng/ml FGF19 10 uM DAPT 3 uM Dexamethasone 3 uM IWP-2 3 uM Chir 50 uM iCRT3 100 uM Carbachol Old differentiation medium (DM) Concentration Component Gibco Advanced DMEM/F12 10 mM HEPES 1 x B27 wo VitA 1 x N2 1.25 mM n-Acetylcysteine 50 ng/ml hEGF 10 nM Gastrin 25 ng/ml HGF 0.5 uM A83.01 25 ng/ml BMP7 100 ng/ml FGF19 10 uM DAPT 3 uM Dexamethasone Expansion medium (EM) Concentration Component Gibco Advanced DMEM/F12 10 mM HEPES 1 x B27 wo VitA 1 x N2 1.25 mM n-Acetylcysteine 50 ng/ml hEGF 10 nM Gastrin 25 ng/ml HGF 10 % Rspondin conditioned medium 0.01 M Nicotinamide 100 ng/ml FGF10 5 uM A83.01 10 uM FSK ..

Cell Culture:

Article Title: Retinoic acid regulates fetoplacental vascularization via notch signaling and a SEMA3E/F-PLEXIND1 axis
Article Snippet: .. Explants were cultured with DMSO for the control, or with retinoic acid (RA) (1 μM) (CAS #302-79-4; Sigma-Aldrich, St. Louis, MO), or DAPT (10 μM) (Cat #AAJ65864MA; Thermo Fisher Scientific, Waltham, MA) for 48 h, then treated with Click-iT® EdU Imaging Kit (Cat# C10337 ; Thermo Fisher Scientific, Waltham, MA) to assess proliferation and fixed by 4% PFA in PBS for 20 min. Allantoides were permeabilized and blocked for 30 min using 0.1% TritonTM X-100 (Cat# T8787; Sigma-Aldrich, St. Louis, MO) with 10% Normal Donkey Serum (NDS) (Cat# 0030-01; Southern Biotech, Birmingham, AL) in PBS. ..

Article Title: An Innervated and Vascularized HNSCC-on-a-Chip Model Built on Defined and Tunable Engineered Extracellular Matrices
Article Snippet: After 14 days of differentiation, the resulting trigeminal precursors were detached using 0.5 mM EDTA and resuspended in a hydrogel containing hyaluronic acid, polyethylene glycol (PEG), and human laminin 521 (Cat. #LN521-05, BioLamina) (see section “Hydrogels composition and fabrication” for details on hydrogels composition). .. Trigeminal precursors were then cultured within the 3D eECM in Neurobasal (Cat. #21103049, ThermoFisher Scientific) supplemented with 2 % B27 (Cat. #17504044, ThermoFisher Scientific), NGF 50 ng/mL (Cat. #450-01-100UG, ThermoFisher Scientific), BDNF 50 ng/mL (Cat. #AF-450-02-10UG, ThermoFisher Scientific; Cat. #78005, STEMCELL Technologies), GDNF 50 ng/mL (Cat. #450-10-10UG, ThermoFisher Scientific; Cat. #78058, STEMCELL Technologies), DAPT 10 μM (Cat. #J65864.MA, ThermoFisher Scientific), and non-essential aminoacids (Cat. #11140050, ThermoFisher Scientific), for up to 40 days to generate human bioengineered trigeminal ganglia (hbTG). .. For calcium imaging, hbTG were infected with AAV9-CAG-jGCaMP8f-WPRE (2.6 * E 11 GC/hbTG; stock titer: 2.6 * E 13 GC/ml, Addgene) on day 34 of differentiation in eECM.

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