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Servicebio Inc diamidino 2 phenylindole dapi staining
Diamidino 2 Phenylindole Dapi Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining/2+4+6+diamidino+phenylindole/pmc13049910-67-22-29
Average 86 stars, based on 1 article reviews
diamidino 2 phenylindole dapi staining - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Staining:

Article Title: P2RY6 + tumor-associated macrophages as a therapeutic target: suppressing tumor growth and enhancing chemosensitivity in triple-negative breast cancer.
Article Snippet: Triple-negative breast cancer (TNBC) is recognized as the most aggressive breast cancer subtype, with a characteristically poor clinical prognosis.. Tumor-associated macrophages (TAMs) are known to impede both antitumor immunity and response to chemotherapy.. In the tumor microenvironment, nucleotides accumulate extracellularly and activate signaling through P2 purinergic receptors, which emerge as pivotal immunomodulatory players in cancer.

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation.
Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), 4',6-diamidino-2-phenylindole (DAPI; Servicebio®, G1012) nuclear counterstaining (10 min), autofluorescence quenching (Servicebio®, G1221) for 5 min, and mounting in anti-fade medium (Servicebio®, G1401). .. Images were captured using a fluorescence microscope (Leica, Germany, Leica STELLARIS8).

Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
Article Snippet: Polysorbate 80 (Tween 80) was obtained from Adamas-Beta Co., Ltd. (Shanghai, China). .. Citrate antigen retrieval buffer (pH 6.0), EDTA antigen retrieval buffers (pH 8.0 and pH 9.0), phosphate-buffered saline (PBS), tissue autofluorescence quencher, and 4′,6-diamidino-2-phenylindole (DAPI) staining reagent were provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. Propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China).

Article Title: An inhaled AK0705 modulates PI3K/AKT/MAPK/NF-κB signaling to alleviate LPS-NETs-induced inflammation and pyroptosis in acute lung injury.
Article Snippet: Neutrophil elastase (NE) plays a crucial role in the progression of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).. AK0705 is a potent small molecule inhaled NE inhibitor (IC50 = 0.015 nM).. Herein, we investigated the protective effect of AK0705 on lipopolysaccharide (LPS)-induced ALI and the mechanism.

End Labeling:

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Incubation:

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation.
Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), 4',6-diamidino-2-phenylindole (DAPI; Servicebio®, G1012) nuclear counterstaining (10 min), autofluorescence quenching (Servicebio®, G1221) for 5 min, and mounting in anti-fade medium (Servicebio®, G1401). .. Images were captured using a fluorescence microscope (Leica, Germany, Leica STELLARIS8).

Fluorescence:

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Microscopy:

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Immunofluorescence:

Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation.
Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), 4',6-diamidino-2-phenylindole (DAPI; Servicebio®, G1012) nuclear counterstaining (10 min), autofluorescence quenching (Servicebio®, G1221) for 5 min, and mounting in anti-fade medium (Servicebio®, G1401). .. Images were captured using a fluorescence microscope (Leica, Germany, Leica STELLARIS8).

Blocking Assay:

Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation.
Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), 4',6-diamidino-2-phenylindole (DAPI; Servicebio®, G1012) nuclear counterstaining (10 min), autofluorescence quenching (Servicebio®, G1221) for 5 min, and mounting in anti-fade medium (Servicebio®, G1401). .. Images were captured using a fluorescence microscope (Leica, Germany, Leica STELLARIS8).

Saline:

Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
Article Snippet: Polysorbate 80 (Tween 80) was obtained from Adamas-Beta Co., Ltd. (Shanghai, China). .. Citrate antigen retrieval buffer (pH 6.0), EDTA antigen retrieval buffers (pH 8.0 and pH 9.0), phosphate-buffered saline (PBS), tissue autofluorescence quencher, and 4′,6-diamidino-2-phenylindole (DAPI) staining reagent were provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. Propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China).



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Image Search Results


Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Journal: Biomaterials and Biosystems

Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

doi: 10.1016/j.bbiosy.2026.100137

Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

Techniques: Staining, Cell Culture