diamidino 2 phenylindole dapi staining (Servicebio Inc)
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Diamidino 2 Phenylindole Dapi Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining/2+4+6+diamidino+phenylindole/pmc13049910-67-22-29
Average 86 stars, based on 1 article reviews
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Staining:Article Title: P2RY6 + tumor-associated macrophages as a therapeutic target: suppressing tumor growth and enhancing chemosensitivity in triple-negative breast cancer. Article Snippet: Triple-negative breast cancer (TNBC) is recognized as the most aggressive breast cancer subtype, with a characteristically poor clinical prognosis.. Tumor-associated macrophages (TAMs) are known to impede both antitumor immunity and response to chemotherapy.. In the tumor microenvironment, nucleotides accumulate extracellularly and activate signaling through P2 purinergic receptors, which emerge as pivotal immunomodulatory players in cancer. Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation. Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota Article Snippet: Polysorbate 80 (Tween 80) was obtained from Adamas-Beta Co., Ltd. (Shanghai, China). .. Citrate antigen retrieval buffer (pH 6.0), EDTA antigen retrieval buffers (pH 8.0 and pH 9.0), phosphate-buffered saline (PBS), tissue autofluorescence quencher, and Article Title: An inhaled AK0705 modulates PI3K/AKT/MAPK/NF-κB signaling to alleviate LPS-NETs-induced inflammation and pyroptosis in acute lung injury. Article Snippet: Neutrophil elastase (NE) plays a crucial role in the progression of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).. AK0705 is a potent small molecule inhaled NE inhibitor (IC50 = 0.015 nM).. Herein, we investigated the protective effect of AK0705 on lipopolysaccharide (LPS)-induced ALI and the mechanism. End Labeling:Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with Incubation:Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation. Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), Fluorescence:Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with Microscopy:Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with Immunofluorescence:Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation. Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), Blocking Assay:Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation. Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), Saline:Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota Article Snippet: Polysorbate 80 (Tween 80) was obtained from Adamas-Beta Co., Ltd. (Shanghai, China). .. Citrate antigen retrieval buffer (pH 6.0), EDTA antigen retrieval buffers (pH 8.0 and pH 9.0), phosphate-buffered saline (PBS), tissue autofluorescence quencher, and |
