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Servicebio Inc diamidino 2 phenylindole dapi staining
Diamidino 2 Phenylindole Dapi Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
diamidino 2 phenylindole dapi staining - by Bioz Stars, 2026-10
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Related Articles

Staining:

Article Title: P2RY6 + tumor-associated macrophages as a therapeutic target: suppressing tumor growth and enhancing chemosensitivity in triple-negative breast cancer.
Article Snippet: Triple-negative breast cancer (TNBC) is recognized as the most aggressive breast cancer subtype, with a characteristically poor clinical prognosis.. Tumor-associated macrophages (TAMs) are known to impede both antitumor immunity and response to chemotherapy.. In the tumor microenvironment, nucleotides accumulate extracellularly and activate signaling through P2 purinergic receptors, which emerge as pivotal immunomodulatory players in cancer.

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation.
Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), 4',6-diamidino-2-phenylindole (DAPI; Servicebio®, G1012) nuclear counterstaining (10 min), autofluorescence quenching (Servicebio®, G1221) for 5 min, and mounting in anti-fade medium (Servicebio®, G1401). .. Images were captured using a fluorescence microscope (Leica, Germany, Leica STELLARIS8).

Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
Article Snippet: Polysorbate 80 (Tween 80) was obtained from Adamas-Beta Co., Ltd. (Shanghai, China). .. Citrate antigen retrieval buffer (pH 6.0), EDTA antigen retrieval buffers (pH 8.0 and pH 9.0), phosphate-buffered saline (PBS), tissue autofluorescence quencher, and 4′,6-diamidino-2-phenylindole (DAPI) staining reagent were provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. Propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China).

Article Title: An inhaled AK0705 modulates PI3K/AKT/MAPK/NF-κB signaling to alleviate LPS-NETs-induced inflammation and pyroptosis in acute lung injury.
Article Snippet: Neutrophil elastase (NE) plays a crucial role in the progression of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).. AK0705 is a potent small molecule inhaled NE inhibitor (IC50 = 0.015 nM).. Herein, we investigated the protective effect of AK0705 on lipopolysaccharide (LPS)-induced ALI and the mechanism.

End Labeling:

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Incubation:

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation.
Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), 4',6-diamidino-2-phenylindole (DAPI; Servicebio®, G1012) nuclear counterstaining (10 min), autofluorescence quenching (Servicebio®, G1221) for 5 min, and mounting in anti-fade medium (Servicebio®, G1401). .. Images were captured using a fluorescence microscope (Leica, Germany, Leica STELLARIS8).

Fluorescence:

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Microscopy:

Article Title: Calcium overload induces reactive oxygen species to enhance glioblastoma radiosensitivity
Article Snippet: The tumor tissues were fixed in 4% paraformaldehyde overnight, dehydrated in 30% sucrose, sectioned (20 μm), stained with hematoxylin (Biosharp, BL702B) for 10 minutes and eosin (Biosharp, BL703B) for 2 minutes, differentiated in 1% acidic ethanol, mounted, and examined under a fluorescence microscope. .. Terminal transferase dUTP nick end labeling staining The tumor sections were incubated with terminal transferase dUTP nick end labeling kit (Heruibio, HRX1204) for 1 hour at 37°C, washed, incubated with streptavidin-tetramethylrhodamine isothiocyanate (Heruibio, Fuzhou, China, HRX1204) for 30 minutes, counterstained with 4′,6-diamidino-2-phenylindole (Servicebio, G1012), and imaged by fluorescence microscopy. ..

Immunofluorescence:

Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation.
Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), 4',6-diamidino-2-phenylindole (DAPI; Servicebio®, G1012) nuclear counterstaining (10 min), autofluorescence quenching (Servicebio®, G1221) for 5 min, and mounting in anti-fade medium (Servicebio®, G1401). .. Images were captured using a fluorescence microscope (Leica, Germany, Leica STELLARIS8).

Blocking Assay:

Article Title: Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammation.
Article Snippet: IHC results were quantified using ImageJ software based on integrated optical density (IOD), analyzing three randomly selected fields per sample. .. Immunofluorescence (IF) staining Paraffin-embedded xenograft tumor/TNBC clinical sections underwent sequential deparaffinization, rehydration, antigen retrieval, peroxidase blocking, species-appropriate serum/BSA blocking, overnight primary antibodies (detailed in Table 1) incubation at 4°C, fluorophore-conjugated secondary antibodies incubation (50 min), 4',6-diamidino-2-phenylindole (DAPI; Servicebio®, G1012) nuclear counterstaining (10 min), autofluorescence quenching (Servicebio®, G1221) for 5 min, and mounting in anti-fade medium (Servicebio®, G1401). .. Images were captured using a fluorescence microscope (Leica, Germany, Leica STELLARIS8).

Saline:

Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
Article Snippet: Polysorbate 80 (Tween 80) was obtained from Adamas-Beta Co., Ltd. (Shanghai, China). .. Citrate antigen retrieval buffer (pH 6.0), EDTA antigen retrieval buffers (pH 8.0 and pH 9.0), phosphate-buffered saline (PBS), tissue autofluorescence quencher, and 4′,6-diamidino-2-phenylindole (DAPI) staining reagent were provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. Propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China).



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LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
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Image Search Results


LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

Journal: International Journal of Nanomedicine

Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

doi: 10.2147/IJN.S645818

Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing