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ZSGB Biotech 40,6-diamidino-2phenylindole (dapi)
40,6 Diamidino 2phenylindole (Dapi), supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining/vectorshield+antifade+media+with+4++6+diamidino+2+phenylindole++dapi++for+staining+cell+nucleus+/pm38572102-74-64-67
Average 90 stars, based on 1 article reviews
40,6-diamidino-2phenylindole (dapi) - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: Proteomic analysis of mouse testis reveals perfluorooctanoic acid-induced reproductive dysfunction via direct disturbance of testicular steroidogenic machinery.
Article Snippet: Perfluorooctanoic acid (PFOA) is a ubiquitous environmental pollutant suspected of being an endocrine disruptor; however, mechanisms of male reproductive disorders induced by PFOA are poorly understood.. In this study, male mice were exposed to 0, 0.31, 1.25, 5, and 20 mg PFOA/kg/day by oral gavage for 28 days.. PFOA significantly damaged the seminiferous tubules and reduced testosterone and progesterone levels in the testis in a dose-dependent manner.

Article Title: The Expression of Interferon Regulatory Factor 8 in Human Periapical Lesions.
Article Snippet: The expression of IRF8 is observed and might possibly be involved in macrophages in the development of periapical lesions.. Our findings provide new clues to the mechanisms of periapical diseases and the treatment of thediseaseby targeting IRF8.. Introduction: Interferon regulatory factor 8 (IRF8) is a critical transcription factor in innate immune responses that regulates the development and function of myeloid cells.

Microscopy:

Article Title: Proteomic analysis of mouse testis reveals perfluorooctanoic acid-induced reproductive dysfunction via direct disturbance of testicular steroidogenic machinery.
Article Snippet: Perfluorooctanoic acid (PFOA) is a ubiquitous environmental pollutant suspected of being an endocrine disruptor; however, mechanisms of male reproductive disorders induced by PFOA are poorly understood.. In this study, male mice were exposed to 0, 0.31, 1.25, 5, and 20 mg PFOA/kg/day by oral gavage for 28 days.. PFOA significantly damaged the seminiferous tubules and reduced testosterone and progesterone levels in the testis in a dose-dependent manner.

Incubation:

Article Title: FGF4 protects the liver from immune-mediated injury by activating CaMKK β -PINK1 signal pathway to inhibit hepatocellular apoptosis.
Article Snippet: .. Following antigen retrieval, the de-paraffinized or frozen liver sections were rinsed with PBS twice, blocked with 5% BSA for 1 h at 25 C, and then incubated with primary antibodies against antigens (Supporting Information Table S4) overnight at 4 C. The sections were then washed thrice with PBS and incubated with fluorescence-conjugated secondary antibodies (Table S4) for 1 h. The nuclei were counterstained with 40,6-diamidino-2phenylindole (DAPI) (ZLI-9018; ZSGB-BIO). .. Fluorescent images were captured under a Nikon C2si confocal microscope and analysed using the ImageJ software.

Article Title: The Expression of Interferon Regulatory Factor 8 in Human Periapical Lesions.
Article Snippet: The expression of IRF8 is observed and might possibly be involved in macrophages in the development of periapical lesions.. Our findings provide new clues to the mechanisms of periapical diseases and the treatment of thediseaseby targeting IRF8.. Introduction: Interferon regulatory factor 8 (IRF8) is a critical transcription factor in innate immune responses that regulates the development and function of myeloid cells.

Fluorescence:

Article Title: FGF4 protects the liver from immune-mediated injury by activating CaMKK β -PINK1 signal pathway to inhibit hepatocellular apoptosis.
Article Snippet: .. Following antigen retrieval, the de-paraffinized or frozen liver sections were rinsed with PBS twice, blocked with 5% BSA for 1 h at 25 C, and then incubated with primary antibodies against antigens (Supporting Information Table S4) overnight at 4 C. The sections were then washed thrice with PBS and incubated with fluorescence-conjugated secondary antibodies (Table S4) for 1 h. The nuclei were counterstained with 40,6-diamidino-2phenylindole (DAPI) (ZLI-9018; ZSGB-BIO). .. Fluorescent images were captured under a Nikon C2si confocal microscope and analysed using the ImageJ software.

other:

Article Title: CREB3L1 deficiency impairs odontoblastic differentiation and molar dentin deposition partially through the TMEM30B
Article Snippet: For single immuno uorescence staining, samples were counterstained with 4’,6-diamidino-2phenylindole (DAPI; ZSGB-BIO, Beijing, China) for visualization of the nuclei.

Article Title: The role of von Willebrand factor in the pathogenesis of C3 glomerulopathy
Article Snippet: Nuclei were stained with 4′,6-diamidino-2phenylindole (ZSGB-Bio).



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Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
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Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
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Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
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Image Search Results


Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Journal: Biomaterials and Biosystems

Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

doi: 10.1016/j.bbiosy.2026.100137

Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

Techniques: Staining, Cell Culture