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nuclear stain dapi  (Promega)

 
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    Structured Review

    Promega nuclear stain dapi
    Nuclear Stain Dapi, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/dapi+staining/pm18728402-228-1-7
    Average 90 stars, based on 1 article reviews
    nuclear stain dapi - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Nm23-H1 modulates the activity of the guanine exchange factor Dbl-1.
    Article Snippet: Rabbit anti-Dbl antibody was purchased from Santa Cruz (Santa Cruz, CA), anti-Nm23 antibody was obtained from SEIKAGAKU Corp. (Tokyo, Japan), anti-Myc antibody 9E10 and anti-HA antibody 12CA5, were culture products of hybridoma cell lines. .. The nuclear stain DAPI was purchased from Promega (Madison, WI). ..

    Article Title: Use of oligosaccharide compounds for the prevention and treatment of pathological scars
    Article Snippet: .. The immunolabeling is carried out using DAPI staining (which gives a blue color) for the cell nuclei, a rabbit anti-α-SMA antibody and an anti-rabbit secondary antibody coupled to Cys3 (sold by Promega). ..

    Article Title: Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells.
    Article Snippet: Article Chronology: Received 27 March 2007 Revised version received 17 August 2007 Accepted 23 August 2007 Available online 5 September 2007 Id-1 (inhibitor of differentiation or DNA binding-1) has been positively associated with cell proliferation, cell cycle progression, and invasiveness during tumorigenesis.. In addition, Id1 has been shown tomodulate cellular sensitivity to TGF-β1 (transforming growth factor β1).. Here we demonstrate a novel role of Id-1 in promoting TGF-β1-induced cell motility in a non-malignant prostate epithelial cell line, NPTX.

    Article Title: The suppressor of metastasis Nm23-H1 interacts with the Cdc42 Rho family member and the pleckstrin homology domain of oncoprotein Dbl-1 to suppress cell migration.
    Article Snippet: Rabbit anti-Dbl antibody was purchased from Santa Cruz (Santa Cruz, CA), anti-Nm23-H1 antibody was obtained from Seikagaku Corp (Tokyo, Japan), anti-Myc antibody 9E10 and anti-HA antibody 12CA5, were obtained from hybridoma cell culture. .. The nuclear stain DAPI was purchased from Promega Inc.(Madison, WI). ..

    Immunolabeling:

    Article Title: Use of oligosaccharide compounds for the prevention and treatment of pathological scars
    Article Snippet: .. The immunolabeling is carried out using DAPI staining (which gives a blue color) for the cell nuclei, a rabbit anti-α-SMA antibody and an anti-rabbit secondary antibody coupled to Cys3 (sold by Promega). ..

    other:

    Article Title: Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging
    Article Snippet: The results were evaluated by DAPI (Promega, Madison, WI, USA) staining and analyzed with a flow cytometer (MACSQuant, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Control:

    Article Title: Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells.
    Article Snippet: Article Chronology: Received 27 March 2007 Revised version received 17 August 2007 Accepted 23 August 2007 Available online 5 September 2007 Id-1 (inhibitor of differentiation or DNA binding-1) has been positively associated with cell proliferation, cell cycle progression, and invasiveness during tumorigenesis.. In addition, Id1 has been shown tomodulate cellular sensitivity to TGF-β1 (transforming growth factor β1).. Here we demonstrate a novel role of Id-1 in promoting TGF-β1-induced cell motility in a non-malignant prostate epithelial cell line, NPTX.

    Western Blot:

    Article Title: Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells.
    Article Snippet: Article Chronology: Received 27 March 2007 Revised version received 17 August 2007 Accepted 23 August 2007 Available online 5 September 2007 Id-1 (inhibitor of differentiation or DNA binding-1) has been positively associated with cell proliferation, cell cycle progression, and invasiveness during tumorigenesis.. In addition, Id1 has been shown tomodulate cellular sensitivity to TGF-β1 (transforming growth factor β1).. Here we demonstrate a novel role of Id-1 in promoting TGF-β1-induced cell motility in a non-malignant prostate epithelial cell line, NPTX.

    Transfection:

    Article Title: Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells.
    Article Snippet: Article Chronology: Received 27 March 2007 Revised version received 17 August 2007 Accepted 23 August 2007 Available online 5 September 2007 Id-1 (inhibitor of differentiation or DNA binding-1) has been positively associated with cell proliferation, cell cycle progression, and invasiveness during tumorigenesis.. In addition, Id1 has been shown tomodulate cellular sensitivity to TGF-β1 (transforming growth factor β1).. Here we demonstrate a novel role of Id-1 in promoting TGF-β1-induced cell motility in a non-malignant prostate epithelial cell line, NPTX.



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    Image Search Results


    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Journal: Biomaterials and Biosystems

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

    doi: 10.1016/j.bbiosy.2026.100137

    Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

    Techniques: Staining, Cell Culture