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Softworx Inc constrained, iterative deconvolution
Constrained, Iterative Deconvolution, supplied by Softworx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/constrained-iterative+deconvolution/constrained+iterative+deconvolution/pmc09631952-105-0-10
Average 90 stars, based on 1 article reviews
constrained, iterative deconvolution - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: A bacterial genotoxin reveals a p53-proteasome-LC3 regulatory axis that drives suppression of autophagy in cells experiencing sublethal DNA damage
Article Snippet: Deconvolution was performed using SoftworX constrained iterative 884 deconvolution tool (ratio mode) and analyzed using Imaris (Version 7.4.2, Bitplane, 885 Switzerland).

Article Title: Vps39 Interacts with Tom40 to Establish One of Two Functionally Distinct Vacuole-Mitochondria Contact Sites.
Article Snippet: Cells were washed twice with water and imaged in water.We used z-stacks with 250 nm spacing for constrained-iterative deconvolution (SoftWoRx), and the images shown are deconvolved images of one z-plane.

Article Title: Trogocytosis Results in Sustained Intracellular Signaling in CD4 + T Cells
Article Snippet: Image analysis Constrained, iterative deconvolution was performed using the API SoftWorx software package.

Article Title: A systematic approach to identify recycling endocytic cargo depending on the GARP complex
Article Snippet: We used constrained-iterative deconvolution (SoftWoRx).

Article Title: The receptor subunit Tom20 is dynamically associated with the TOM complex in mitochondria of human cells
Article Snippet: We used z-stacks with 250 nm spacing for constrained-iterative deconvolution (SoftWoRx).

Article Title: Rab7 regulates primary cilia disassembly through cilia excision
Article Snippet: In our original manuscript, in order to show cilia clearly, the representative images in Fig. 3A were processed by iterative constrained deconvolution (SoftWoRx, Applied Precision Instruments).

Article Title: A bacterial genotoxin reveals a p53-proteasome-LC3 regulatory axis that drives the suppression of autophagy in cells experiencing sublethal DNA damage
Article Snippet: Deconvolution was performed using SoftworX constrained iterative deconvolution tool (ratio mode) and analyzed using Imaris (Version 7.4.2, Bitplane, Switzerland).

Microscopy:

Article Title: Ablation of SUN2-containing LINC complexes drives cardiac hypertrophy without interstitial fibrosis
Article Snippet: Cardiac sections processed for immunofluorescence were imaged on a Leica SP5 confocal microscope or a widefield deconvolution microscope (DeltaVision; Applied Precision/GE Healthcare) with a charge-coupled device (CCD) camera (CoolSNAP K4; Photometrics) and SoftWoRx software. .. All images acquired on the DeltaVision microscope were deconvolved using the Deconvolve tool (constrained iterative deconvolution) in SoftWoRx software. .. The DeltaVision microscope was equipped with an oil Plan Apochromat N 60×/1.42 NA objective (Olympus).



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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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https://www.bioz.com/product/constrained-iterative+deconvolution/constrained+iterative+deconvolution/pmc09631952-105-0-10
Average 90 stars, based on 1 article reviews
constrained, iterative deconvolution - by Bioz Stars, 2026-09
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

Journal: American journal of respiratory cell and molecular biology

Article Title: Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen Challenged Mice

doi: 10.1165/rcmb.2004-0060OC

Figure Lengend Snippet: Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

Article Snippet: Tissues were observed on an inverted microscope by focusing on 45 consecutive 0.2 μm focal planes followed by constrained-iterative deconvolution (DeltaVision, Applied Precision, LLC, Issaquah, Washington).

Techniques: Produced, Staining, Immunolabeling, Microscopy