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the constrained iterative deconvolution algorithm  (Evident Corporation)

 
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    Evident Corporation the constrained iterative deconvolution algorithm
    The Constrained Iterative Deconvolution Algorithm, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constrained-iterative+deconvolution/constrained+iterative+deconvolution+algorithm/pm36869698-38-7-10
    Average 90 stars, based on 1 article reviews
    the constrained iterative deconvolution algorithm - by Bioz Stars, 2026-09
    90/100 stars

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    Marker:

    Article Title: The Defenders of the Alveolus Succumb in COVID-19 Pneumonia to SARS-CoV-2 and Necroptosis, Pyroptosis, and PANoptosis.
    Article Snippet: Monochrome images for fluorescence in situ hybridization (FISH) and immunofluorescence-stained slides were captured for each fluorophore using an Olympus DP80. .. A pseudocolor was applied using the constrained iterative deconvolution algorithm (Olympus) for each marker and fused into a composite image for viewing. .. Image processing and analysis for IHC- and ISH-stained slides were performed using the area quantification and deconvolution modules of Halo image analysis platform (Indica Labs, Inc).



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    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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    Image Search Results


    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Journal: American journal of respiratory cell and molecular biology

    Article Title: Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen Challenged Mice

    doi: 10.1165/rcmb.2004-0060OC

    Figure Lengend Snippet: Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Article Snippet: Tissues were observed on an inverted microscope by focusing on 45 consecutive 0.2 μm focal planes followed by constrained-iterative deconvolution (DeltaVision, Applied Precision, LLC, Issaquah, Washington).

    Techniques: Produced, Staining, Immunolabeling, Microscopy