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constrained iterative deconvolution routines  (Carl Zeiss)


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    Carl Zeiss constrained iterative deconvolution routines
    Constrained Iterative Deconvolution Routines, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constrained-iterative+deconvolution/200m+axiovert+microscope/pmc09220948-262-8-15
    Average 90 stars, based on 1 article reviews
    constrained iterative deconvolution routines - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Time-lapse Microscopy:

    Article Title: Protocol for induction and study of DNA double-strand breaks in mammalian cells using PALM microdissection and expansion microscopy
    Article Snippet: .. 3D reconstruction of a cell to analyze protein distribution across nuclear planes, related to materials and equipment setup Besides, to confirm that laser microirradiation did not compromise cell viability, a living cells- time-lapse microscopy assay was performed after microirradiation on HeLa-FUCCI cells using a Zeiss AxioVert 200M inverted microscope coupled with a XL-LXM 710 S1 PEACON live-cell incubation chamber. ..

    Inverted Microscopy:

    Article Title: Protocol for induction and study of DNA double-strand breaks in mammalian cells using PALM microdissection and expansion microscopy
    Article Snippet: .. 3D reconstruction of a cell to analyze protein distribution across nuclear planes, related to materials and equipment setup Besides, to confirm that laser microirradiation did not compromise cell viability, a living cells- time-lapse microscopy assay was performed after microirradiation on HeLa-FUCCI cells using a Zeiss AxioVert 200M inverted microscope coupled with a XL-LXM 710 S1 PEACON live-cell incubation chamber. ..

    Article Title: Protocol for induction and study of DNA double-strand breaks in mammalian cells using PALM microdissection and expansion microscopy
    Article Snippet: LSM 710 confocal inverted microscope , Zeiss , RRID: SCR_018063. .. AxioVert 200M inverted microscope , Zeiss , RRID: SCR_020915. .. Microscope power sensor head , Thorlabs , S175C.

    Incubation:

    Article Title: Protocol for induction and study of DNA double-strand breaks in mammalian cells using PALM microdissection and expansion microscopy
    Article Snippet: .. 3D reconstruction of a cell to analyze protein distribution across nuclear planes, related to materials and equipment setup Besides, to confirm that laser microirradiation did not compromise cell viability, a living cells- time-lapse microscopy assay was performed after microirradiation on HeLa-FUCCI cells using a Zeiss AxioVert 200M inverted microscope coupled with a XL-LXM 710 S1 PEACON live-cell incubation chamber. ..

    Article Title: Semaphorin 3F is elevated in serum of heart failure patients and inhibits cardiac angiogenesis via the VEGF/Akt/eNOS pathway
    Article Snippet: .. Spheroids were embedded in collagen gels and incubated with the indicated amounts of recombinant Sema3F and/or VEGF for 24 h. Spheroids were imaged with an Axiovert 25 microscope (Carl Zeiss GmbH, Jena Germany) using the AxioVision Rel. ..

    Article Title: Maltodextrin-modified lipoplexes for enhanced mucosal penetration and efficient mRNA delivery
    Article Snippet: .. The cells were then incubated at 37 °C for specific time intervals (15, 20, 40, 60, and 120 min) and subsequently visualized using a Zeiss Axiovert 200 M microscope coupled with a Zeiss LSM 980 airyscan 2 (Carl Zeiss Co., Ltd., Iena, Germany). ..

    Control:

    Article Title: Breaking free from PFAS: biocompatible, durable and high-performance octenyl succinic anhydride (OSA)-modified starch/chitosan coating with ZnO for textile applications.
    Article Snippet: The development of textile coatings as alternatives to perand polyfluoroalkyl substances (PFAS) is a high priority due to global regulatory efforts aiming to phase out PFAS, driven by alarming environmental contamination and significant human health concerns.. To address the urgent need for replacements, this research develops a health-friendly hydrophobic coating for two of the most common textile substrates, cotton and polyester.. The developed coating, consisting of chitosan matrix and octenyl succinic anhydride-modified starch in synergy with ZnO, achieved water contact angles up to 132.

    Microscopy:

    Article Title: Breaking free from PFAS: biocompatible, durable and high-performance octenyl succinic anhydride (OSA)-modified starch/chitosan coating with ZnO for textile applications.
    Article Snippet: The development of textile coatings as alternatives to perand polyfluoroalkyl substances (PFAS) is a high priority due to global regulatory efforts aiming to phase out PFAS, driven by alarming environmental contamination and significant human health concerns.. To address the urgent need for replacements, this research develops a health-friendly hydrophobic coating for two of the most common textile substrates, cotton and polyester.. The developed coating, consisting of chitosan matrix and octenyl succinic anhydride-modified starch in synergy with ZnO, achieved water contact angles up to 132.

    Article Title: Semaphorin 3F is elevated in serum of heart failure patients and inhibits cardiac angiogenesis via the VEGF/Akt/eNOS pathway
    Article Snippet: .. Spheroids were embedded in collagen gels and incubated with the indicated amounts of recombinant Sema3F and/or VEGF for 24 h. Spheroids were imaged with an Axiovert 25 microscope (Carl Zeiss GmbH, Jena Germany) using the AxioVision Rel. ..

    Article Title: Maltodextrin-modified lipoplexes for enhanced mucosal penetration and efficient mRNA delivery
    Article Snippet: .. The cells were then incubated at 37 °C for specific time intervals (15, 20, 40, 60, and 120 min) and subsequently visualized using a Zeiss Axiovert 200 M microscope coupled with a Zeiss LSM 980 airyscan 2 (Carl Zeiss Co., Ltd., Iena, Germany). ..

    Article Title: Regulation of airway remodeling in asthma: The synergistic effects of nanotechnology and Hippo pathway activation
    Article Snippet: .. The stained area of each paraffin-embedded section (blue) was outlined using an Axiovert 200 M optical microscope (Carl Zeiss, Jena, Germany), and then Image-Pro Plus software (Version X; Adobe, San Jose, CA) was used to quantify the area occupied by collagen fibers (blue) as a percentage of the total examined area. .. To assess the systemic toxicity of nanoparticles, serum biochemical parameters were measured by collecting blood from mice and using an automated biochemical analyzer (Hitachi, Japan) to evaluate levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (Cr).

    Article Title: Tunable pH-responsive HMSNs-g-PDEAEMA as integrated corrosion inhibition and emulsification multi-functional nanocontainer
    Article Snippet: Responsive nanocontainers have dual functions in targeted delivery of corrosion inhibitors and emulsion development of shale oil in oil and gas fields, exhibiting potential for simultaneously achieving metal protection and efficient oil and gas development from a material perspective.. Here, we propose the preparation of a pH-responsive nanocontainer, HMSNs-g-PDEAEMA (poly [2-(N, N–diethyl amino)ethyl-methacrylate] (PDEAEMA) grafted onto hollow mesoporous spherical silica (HMSNs)), to integrate the delivery of 2-mercaptobenzothiazole (MBT)) for targeted corrosion inhibition and the emulsification of oil as Pickering emulsifiers.. Under acidic conditions (reduced pH value caused by localized corrosion or high concentration acidic gases), PDEAEMA chains are protonated and extended by electrostatic repulsion, exposing pores on HMSNs surface and allowing the controlled release of loaded MBT molecules.

    Recombinant:

    Article Title: Semaphorin 3F is elevated in serum of heart failure patients and inhibits cardiac angiogenesis via the VEGF/Akt/eNOS pathway
    Article Snippet: .. Spheroids were embedded in collagen gels and incubated with the indicated amounts of recombinant Sema3F and/or VEGF for 24 h. Spheroids were imaged with an Axiovert 25 microscope (Carl Zeiss GmbH, Jena Germany) using the AxioVision Rel. ..

    other:

    Article Title: Capacitive and dielectric behavior of Ce substituted BFO {Bi1−xCexFeO3 (0 ≤ x ≤ 0.15)} for electronics packaging applications
    Article Snippet: The substitution of Ce at the Bismuth site of the bismuth ferrite and its effect on the capacitive, resistive and dielectric behavior has been investigated.. The samples synthesized by solid state reaction technique affirms the formation of perovskite phases with presence of impurity phases Bi2Fe4O9 of Bi25FeO40.. At room temperature, the dielectric constant of Bi1− xCexFeO3 is observed to be 125, 111 and 119 for x = 0.05, 0.1 and 0.15 respectively, whereas the dielectric loss values for the above samples are 0.15, 0.20 and 0.135, respectively.

    Staining:

    Article Title: Regulation of airway remodeling in asthma: The synergistic effects of nanotechnology and Hippo pathway activation
    Article Snippet: .. The stained area of each paraffin-embedded section (blue) was outlined using an Axiovert 200 M optical microscope (Carl Zeiss, Jena, Germany), and then Image-Pro Plus software (Version X; Adobe, San Jose, CA) was used to quantify the area occupied by collagen fibers (blue) as a percentage of the total examined area. .. To assess the systemic toxicity of nanoparticles, serum biochemical parameters were measured by collecting blood from mice and using an automated biochemical analyzer (Hitachi, Japan) to evaluate levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (Cr).

    Software:

    Article Title: Regulation of airway remodeling in asthma: The synergistic effects of nanotechnology and Hippo pathway activation
    Article Snippet: .. The stained area of each paraffin-embedded section (blue) was outlined using an Axiovert 200 M optical microscope (Carl Zeiss, Jena, Germany), and then Image-Pro Plus software (Version X; Adobe, San Jose, CA) was used to quantify the area occupied by collagen fibers (blue) as a percentage of the total examined area. .. To assess the systemic toxicity of nanoparticles, serum biochemical parameters were measured by collecting blood from mice and using an automated biochemical analyzer (Hitachi, Japan) to evaluate levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (Cr).

    Electron Microscopy:

    Article Title: Tunable pH-responsive HMSNs-g-PDEAEMA as integrated corrosion inhibition and emulsification multi-functional nanocontainer
    Article Snippet: Responsive nanocontainers have dual functions in targeted delivery of corrosion inhibitors and emulsion development of shale oil in oil and gas fields, exhibiting potential for simultaneously achieving metal protection and efficient oil and gas development from a material perspective.. Here, we propose the preparation of a pH-responsive nanocontainer, HMSNs-g-PDEAEMA (poly [2-(N, N–diethyl amino)ethyl-methacrylate] (PDEAEMA) grafted onto hollow mesoporous spherical silica (HMSNs)), to integrate the delivery of 2-mercaptobenzothiazole (MBT)) for targeted corrosion inhibition and the emulsification of oil as Pickering emulsifiers.. Under acidic conditions (reduced pH value caused by localized corrosion or high concentration acidic gases), PDEAEMA chains are protonated and extended by electrostatic repulsion, exposing pores on HMSNs surface and allowing the controlled release of loaded MBT molecules.

    Spectroscopy:

    Article Title: Tunable pH-responsive HMSNs-g-PDEAEMA as integrated corrosion inhibition and emulsification multi-functional nanocontainer
    Article Snippet: Responsive nanocontainers have dual functions in targeted delivery of corrosion inhibitors and emulsion development of shale oil in oil and gas fields, exhibiting potential for simultaneously achieving metal protection and efficient oil and gas development from a material perspective.. Here, we propose the preparation of a pH-responsive nanocontainer, HMSNs-g-PDEAEMA (poly [2-(N, N–diethyl amino)ethyl-methacrylate] (PDEAEMA) grafted onto hollow mesoporous spherical silica (HMSNs)), to integrate the delivery of 2-mercaptobenzothiazole (MBT)) for targeted corrosion inhibition and the emulsification of oil as Pickering emulsifiers.. Under acidic conditions (reduced pH value caused by localized corrosion or high concentration acidic gases), PDEAEMA chains are protonated and extended by electrostatic repulsion, exposing pores on HMSNs surface and allowing the controlled release of loaded MBT molecules.



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    Image Search Results


    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Journal: American journal of respiratory cell and molecular biology

    Article Title: Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen Challenged Mice

    doi: 10.1165/rcmb.2004-0060OC

    Figure Lengend Snippet: Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Article Snippet: Tissues were observed on an inverted microscope by focusing on 45 consecutive 0.2 μm focal planes followed by constrained-iterative deconvolution (DeltaVision, Applied Precision, LLC, Issaquah, Washington).

    Techniques: Produced, Staining, Immunolabeling, Microscopy