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constrained iterative deconvolution algorithm  (Carl Zeiss)


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    Structured Review

    Carl Zeiss constrained iterative deconvolution algorithm
    Constrained Iterative Deconvolution Algorithm, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constrained-iterative+deconvolution/deconvolution+filter+iterative/pm38747543-67-11-14
    Average 90 stars, based on 1 article reviews
    constrained iterative deconvolution algorithm - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Software:

    Article Title: Quiescent cell re-entry is limited by macroautophagy-induced lysosomal damage.
    Article Snippet: Three dimensional images were subjected to Airyscan Filtering strength 1.0 and subsequently deconvolved using Zeiss’ ‘‘Fast Iterative’’ deconvolution algorithm as part of its Zen 3.1 software. .. Three dimensional images were subjected to Airyscan Filtering strength 1.0 and subsequently deconvolved using Zeiss’ ‘‘Fast Iterative’’ deconvolution algorithm as part of its Zen 3.1 software. ..

    Article Title: Nanoscale dendritic shaft constrictions shape synaptic integration in fine caliber principal neuron dendrites
    Article Snippet: .. The images underwent further processing using the Airyscan processing and joint deconvolution with 10 iterations, as provided by Zeiss ZEN blue 3.6 software. ..

    Article Title: Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies.
    Article Snippet: Article Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies

    Super-Resolution Microscopy:

    Article Title: Microtubule Association of EML4–ALK V3 Is Key for the Elongated Cell Morphology and Enhanced Migration Observed in V3 Cells
    Article Snippet: Nuclei were stained with Hoechst 33258 (0.5 μg/mL, H3569, Invitrogen). .. For super-resolution microscopy, single optical sections were captured in SR mode on a Zeiss LSM980 Airyscan 2 microscope (Zeiss, Oberkochen, Germany) fitted with a PlanApochromat 63× oil objective (NA = 1.4) and further processed using the Zeiss Airyscan joint deconvolution module using 4 iterations. .. Lower-resolution confocal images were captured on a VisiTech Infinity 3 confocal microscope fitted with a Hamamatsu C1144022CU Flash 4.0 V2 sCMOS camera, and a Plan Apo 20× objective (NA = 0.75) was used.

    Microscopy:

    Article Title: Microtubule Association of EML4–ALK V3 Is Key for the Elongated Cell Morphology and Enhanced Migration Observed in V3 Cells
    Article Snippet: Nuclei were stained with Hoechst 33258 (0.5 μg/mL, H3569, Invitrogen). .. For super-resolution microscopy, single optical sections were captured in SR mode on a Zeiss LSM980 Airyscan 2 microscope (Zeiss, Oberkochen, Germany) fitted with a PlanApochromat 63× oil objective (NA = 1.4) and further processed using the Zeiss Airyscan joint deconvolution module using 4 iterations. .. Lower-resolution confocal images were captured on a VisiTech Infinity 3 confocal microscope fitted with a Hamamatsu C1144022CU Flash 4.0 V2 sCMOS camera, and a Plan Apo 20× objective (NA = 0.75) was used.

    other:

    Article Title: Archaeal type six secretion system mediates contact-dependent antagonism
    Article Snippet: Collected confocal stacks were deconvolved with the Zeiss LSM Plus processing function, and the Airyscan images were processed using Zeiss deconvolution (jDCV, 10 iterations) in ZenBlue (v.3.5).

    Article Title: Balanced synapse-to-synapse short-term plasticity ensures constant transmitter release.
    Article Snippet: Airyscan processing was conducted using Zeiss Joint Deconvolution (jDCV) software.



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    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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    Carl Zeiss constrained iterative deconvolution routines
    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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    Image Search Results


    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Journal: American journal of respiratory cell and molecular biology

    Article Title: Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen Challenged Mice

    doi: 10.1165/rcmb.2004-0060OC

    Figure Lengend Snippet: Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Article Snippet: Tissues were observed on an inverted microscope by focusing on 45 consecutive 0.2 μm focal planes followed by constrained-iterative deconvolution (DeltaVision, Applied Precision, LLC, Issaquah, Washington).

    Techniques: Produced, Staining, Immunolabeling, Microscopy