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human 1 cdna microarray slides  (Agilent technologies)


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    Structured Review

    Agilent technologies human 1 cdna microarray slides
    Human 1 Cdna Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+slides/pmc02648774-120-9-14
    Average 90 stars, based on 1 article reviews
    human 1 cdna microarray slides - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: A de novo 13q31.3 microduplication encompassing the miR-17 ~ 92 cluster results in features mirroring those associated with Feingold syndrome 2.
    Article Snippet: Hemizygosity of the MIR17HG gene encoding the miR-17 ~ 92 cluster is associated with Feingold syndrome 2 characterized by intellectual disability, skeletal abnormalities, short stature, and microcephaly.. Here, we report on a female with a de novo 13q31.3 microduplication encompassing MIR17HG but excluding GPC5.. She presented developmental delay, skeletal and digital abnormalities, and features such as tall stature and macrocephaly mirroring those of Feingold syndrome 2 patients.

    DNA Extraction:

    Article Title: A de novo 13q31.3 microduplication encompassing the miR-17 ~ 92 cluster results in features mirroring those associated with Feingold syndrome 2.
    Article Snippet: Hemizygosity of the MIR17HG gene encoding the miR-17 ~ 92 cluster is associated with Feingold syndrome 2 characterized by intellectual disability, skeletal abnormalities, short stature, and microcephaly.. Here, we report on a female with a de novo 13q31.3 microduplication encompassing MIR17HG but excluding GPC5.. She presented developmental delay, skeletal and digital abnormalities, and features such as tall stature and macrocephaly mirroring those of Feingold syndrome 2 patients.

    Generated:

    Article Title: MAID : An effect size based model for microarray data integration across laboratories and platforms
    Article Snippet: These datasets were collected using two different versions of Agilent array technology. .. One dataset was generated using two-channel Agilent Human 1 cDNA array platform containing 12,814 probes. ..

    Microarray:

    Article Title: Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies
    Article Snippet: .. The second microarray [ ] used Agilent Human 1 A oligo assay (Agilent Technologies Inc, Palo Alto, CA, USA) to analyze RNA expression and was available in GEO at ascension number GSE 1818 ( http:ww.ncbi.gov.gen/query/ace.cgi?acc.GSE 1818) and included three samples of NT, three samples of GCNIS, three samples of SE, five samples of EC, four samples of YST, four samples of YST, and one sample of CC. ..

    Article Title: Advanced analysis and visualization of gene copy number and expression data
    Article Snippet: .. The CGH experiments were performed with cDNA arrays on Human 1 cDNA microarray slides (Agilent Technologies, Palo Alto, CA, USA). ..

    Article Title: CGHpower: exploring sample size calculations for chromosomal copy number experiments
    Article Snippet: .. They will be referred to as Chin et al. [ ], Douglas et al. [ ], Fridlyand et al. [ ], Myllykangas et al. [ ], Nymark et al. [ ], Postma et al. [ ], Smeets et al. [ ] and Wrage et al. [ ] A total of five different array types were used among the data sets: VUmc 30 K spotted oligo [ ] for data sets [ , , ], Agilent Human 1 cDNA Microarray for [ , ], 3 K BAC array [ ] for [ ], 2 K BAC array [ ] for [ ] and 6 K BAC array for [ ]. ..

    Article Title: Redefinition of Affymetrix probe sets by sequence overlap with cDNA microarray probes reduces cross-platform inconsistencies in cancer-associated gene expression measurements
    Article Snippet: .. In a recent study, we compared several Affymetrix platforms (for which probe sequence information was available) to the Agilent Human 1 cDNA microarray platform [ ]. .. Probe sequence information was unavailable for the Agilent platform except for a 100 base lead sequence at one end of each cDNA probe.

    Article Title: Identification of Tumor Suppressors and Oncogenes from Genomic and Epigenetic Features in Ovarian Cancer
    Article Snippet: .. Copy number variation data for primary ovarian tumors was downloaded from TCGA ( http://tcga.cancer.gov/ ) and CBS data files from the Agilent SurePrint G3 Human 1 M CGH (Comparative Genomic Hybridization) Microarray with the label mskcc.org_OV.CGH-1×1M_G4447A were analyzed. ..

    RNA Expression:

    Article Title: Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies
    Article Snippet: .. The second microarray [ ] used Agilent Human 1 A oligo assay (Agilent Technologies Inc, Palo Alto, CA, USA) to analyze RNA expression and was available in GEO at ascension number GSE 1818 ( http:ww.ncbi.gov.gen/query/ace.cgi?acc.GSE 1818) and included three samples of NT, three samples of GCNIS, three samples of SE, five samples of EC, four samples of YST, four samples of YST, and one sample of CC. ..

    Labeling:

    Article Title: Improving the statistical detection of regulated genes from microarray data using intensity-based variance estimation
    Article Snippet: .. Briefly, 20 μg of total RNA were direct-labeled with Cy-3 and Cy-5, and labeled cDNAs were hybridized overnight to Agilent Human 1 cDNA arrays (G4100a, Agilent Technologies, Palo Alto, CA) containing 16,142 features representing approximately 10,500 unique genes. .. After washing, the microarrays were scanned in an Agilent model G2505A microarray scanner.

    BAC Assay:

    Article Title: CGHpower: exploring sample size calculations for chromosomal copy number experiments
    Article Snippet: .. They will be referred to as Chin et al. [ ], Douglas et al. [ ], Fridlyand et al. [ ], Myllykangas et al. [ ], Nymark et al. [ ], Postma et al. [ ], Smeets et al. [ ] and Wrage et al. [ ] A total of five different array types were used among the data sets: VUmc 30 K spotted oligo [ ] for data sets [ , , ], Agilent Human 1 cDNA Microarray for [ , ], 3 K BAC array [ ] for [ ], 2 K BAC array [ ] for [ ] and 6 K BAC array for [ ]. ..

    Sequencing:

    Article Title: Redefinition of Affymetrix probe sets by sequence overlap with cDNA microarray probes reduces cross-platform inconsistencies in cancer-associated gene expression measurements
    Article Snippet: .. In a recent study, we compared several Affymetrix platforms (for which probe sequence information was available) to the Agilent Human 1 cDNA microarray platform [ ]. .. Probe sequence information was unavailable for the Agilent platform except for a 100 base lead sequence at one end of each cDNA probe.

    Hybridization:

    Article Title: Identification of Tumor Suppressors and Oncogenes from Genomic and Epigenetic Features in Ovarian Cancer
    Article Snippet: .. Copy number variation data for primary ovarian tumors was downloaded from TCGA ( http://tcga.cancer.gov/ ) and CBS data files from the Agilent SurePrint G3 Human 1 M CGH (Comparative Genomic Hybridization) Microarray with the label mskcc.org_OV.CGH-1×1M_G4447A were analyzed. ..



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    Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) <t>Microarray</t> profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.
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    Image Search Results


    Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Journal: Scientific Reports

    Article Title: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

    doi: 10.1038/srep20395

    Figure Lengend Snippet: Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Article Snippet: Microarray analyses were performed using commercial Mouse cDNA Microarray slides (Phalanx Biotech Group; Hsinchu, Taiwan) according to the manufacturer’s instructions.

    Techniques: Microarray, Software, Western Blot, In Situ, Fluorescence, Staining