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cdna microarray slides  (NimbleGen Systems GmbH)

 
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    NimbleGen Systems GmbH cdna microarray slides
    Cdna Microarray Slides, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+slides/custom+microarrays/pmc04526715-264-13-22
    Average 90 stars, based on 1 article reviews
    cdna microarray slides - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Vibrio cholerae CsrA Regulates ToxR Levels in Response to Amino Acids and Is Essential for Virulence
    Article Snippet: For microarray analysis, approximately 20 g RNA per sample was used to generate cDNA for hybridization to V. cholerae NimbleChip_X4 microarray slides (NimbleGen Systems, Roche Diagnostics Operations, Inc., Indianapolis, IN).

    Article Title: An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
    Article Snippet: Total RNA from early stationary phases between the two isolates were compared using custom Nimblegen Systems microarray chips.

    Article Title: Vibrio cholerae CsrA Regulates ToxR Levels in Response to Amino Acids and Is Essential for Virulence
    Article Snippet: For microarray analysis, approximately 20 μg RNA per sample was used to generate cDNA for hybridization to V. cholerae NimbleChip_X4 microarray slides (NimbleGen Systems, Roche Diagnostics Operations, Inc., Indianapolis, IN).

    Article Title: Listeria monocytogenes Differential Transcriptome Analysis Reveals Temperature-Dependent Agr Regulation and Suggests Overlaps with Other Regulons
    Article Snippet: A custom whole-genome microarray including all the 2857 annotated open reading frames (ORFs) of the genome of Listeria monocytogenes EGD-e was designed by NimbleGen Systems.

    Article Title: Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation
    Article Snippet: A customized whole-genome microarray including the 2,857 annotated open reading frames (ORFs) of the genome of L. monocytogenes EGD-e was designed by NimbleGen Systems.

    Article Title: THE MOUSE RANKL GENE LOCUS IS DEFINED BY A BROAD PATTERN OF HISTONE ACETYLATION AND REGULATED THROUGH DISTINCT DISTAL ENHANCERS
    Article Snippet: These samples were then labeled according to the manufacturer’s ChIP-chip labeling recommendation (NimbleGen Systems, Madison, WI).

    Article Title: Identification and Characterization of a Novel Diterpene Gene Cluster in Aspergillus nidulans
    Article Snippet: The quality of RNA was assessed with the standard protocol of Agilent 2100 Bioanalyzer (Agilent Technologies).

    Microarray:

    Article Title: Succinic Semialdehyde Promotes Prosurvival Capability of Agrobacterium tumefaciens
    Article Snippet: The cDNA molecules were then fragmented into 50 to 200 nucleotides by partial digestion using DNase I (Promega) and labeled with biotin for microarray hybridization. .. The DNA microarray chips were synthesized by NimbleGen Systems (Madison, WI), and each chip included 4,661 open reading frames (ORFs) of the compiled genome sequence of A. tumefaciens strain C58 (University of Washington). ..

    Synthesized:

    Article Title: Succinic Semialdehyde Promotes Prosurvival Capability of Agrobacterium tumefaciens
    Article Snippet: The cDNA molecules were then fragmented into 50 to 200 nucleotides by partial digestion using DNase I (Promega) and labeled with biotin for microarray hybridization. .. The DNA microarray chips were synthesized by NimbleGen Systems (Madison, WI), and each chip included 4,661 open reading frames (ORFs) of the compiled genome sequence of A. tumefaciens strain C58 (University of Washington). ..

    Sequencing:

    Article Title: Succinic Semialdehyde Promotes Prosurvival Capability of Agrobacterium tumefaciens
    Article Snippet: The cDNA molecules were then fragmented into 50 to 200 nucleotides by partial digestion using DNase I (Promega) and labeled with biotin for microarray hybridization. .. The DNA microarray chips were synthesized by NimbleGen Systems (Madison, WI), and each chip included 4,661 open reading frames (ORFs) of the compiled genome sequence of A. tumefaciens strain C58 (University of Washington). ..



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    Image Search Results


    Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Journal: Scientific Reports

    Article Title: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

    doi: 10.1038/srep20395

    Figure Lengend Snippet: Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Article Snippet: Microarray analyses were performed using commercial Mouse cDNA Microarray slides (Phalanx Biotech Group; Hsinchu, Taiwan) according to the manufacturer’s instructions.

    Techniques: Microarray, Software, Western Blot, In Situ, Fluorescence, Staining