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distal tubule marker calbindin d28k  (Proteintech)


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    Structured Review

    Proteintech distal tubule marker calbindin d28k
    Distal Tubule Marker Calbindin D28k, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calbindin/Calbindin-D28k+Antibody/pm41916963-367-33-39
    Average 94 stars, based on 57 article reviews
    distal tubule marker calbindin d28k - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Embigin is involved in the regulation of early mouse kidney development
    Article Snippet: .. Following antibodies were used in our studies: Embigin Monoclonal Antibody, clone G7.43.1, 14-5839-81, eBioscience, Thermo Fisher Scientific (western blotting 1:1000, immunofluorescence 1:200); Monoclonal Anti-β-Tubulin I antibody produced in mouse, clone SAP.4G, T7816, Sigma-Aldrich (western blotting 1:20 000); E-Cadherin Antibody, AF748, R&D Systems (immunofluorescence 1:500); Calbindin D28K Antibody (C-20), sc-7691, Santa Cruz Biotechnology (immunofluorescence 1:400); Six2 Polyclonal antibody, 11562-1-AP, ProteinTech (immunofluorescence 1:500); Anti-Monocarboxylate Transporter 1 Antibody, AB1286-I, Sigma-Aldrich (immunofluorescence 1:1000); Anti-Fibronectin Antibody, AB2033, Sigma-Aldrich (immunofluorescence 1:80); IRDye secondary antibodies, LI-COR Biosciences (western blotting 1:15 000); and Alexa Fluor secondary antibodies, Thermo Fisher Scientific (immunofluorescence 1:400). ..

    Article Title: Embigin is involved in the regulation of early mouse kidney development.
    Article Snippet: .. AR TIC LE IN PR ES S Antibodies Following antibodies were used in our studies: Embigin Monoclonal Antibody, clone G7.43.1, 14-5839-81, eBioscience, Thermo Fisher Scientific (western blotting 1:1000, immunofluorescence 1:200); Monoclonal Anti-β-Tubulin I antibody produced in mouse, clone SAP.4G, T7816, Sigma-Aldrich (western blotting 1:20 000); E-Cadherin Antibody, AF748, R&D Systems (immunofluorescence 1:500); Calbindin D28K Antibody (C-20), sc-7691, Santa Cruz Biotechnology (immunofluorescence 1:400); Six2 Polyclonal antibody, 11562-1-AP, ProteinTech (immunofluorescence 1:500); AntiMonocarboxylate Transporter 1 Antibody, AB1286-I, Sigma-Aldrich (immunofluorescence 1:1000); Anti-Fibronectin Antibody, AB2033, Sigma-Aldrich (immunofluorescence 1:80); IRDye secondary antibodies, LI-COR Biosciences (western blotting 1:15 000); and Alexa Fluor secondary antibodies, Thermo Fisher Scientific (immunofluorescence 1:400). ..

    Immunofluorescence:

    Article Title: Embigin is involved in the regulation of early mouse kidney development
    Article Snippet: .. Following antibodies were used in our studies: Embigin Monoclonal Antibody, clone G7.43.1, 14-5839-81, eBioscience, Thermo Fisher Scientific (western blotting 1:1000, immunofluorescence 1:200); Monoclonal Anti-β-Tubulin I antibody produced in mouse, clone SAP.4G, T7816, Sigma-Aldrich (western blotting 1:20 000); E-Cadherin Antibody, AF748, R&D Systems (immunofluorescence 1:500); Calbindin D28K Antibody (C-20), sc-7691, Santa Cruz Biotechnology (immunofluorescence 1:400); Six2 Polyclonal antibody, 11562-1-AP, ProteinTech (immunofluorescence 1:500); Anti-Monocarboxylate Transporter 1 Antibody, AB1286-I, Sigma-Aldrich (immunofluorescence 1:1000); Anti-Fibronectin Antibody, AB2033, Sigma-Aldrich (immunofluorescence 1:80); IRDye secondary antibodies, LI-COR Biosciences (western blotting 1:15 000); and Alexa Fluor secondary antibodies, Thermo Fisher Scientific (immunofluorescence 1:400). ..

    Article Title: Embigin is involved in the regulation of early mouse kidney development.
    Article Snippet: .. AR TIC LE IN PR ES S Antibodies Following antibodies were used in our studies: Embigin Monoclonal Antibody, clone G7.43.1, 14-5839-81, eBioscience, Thermo Fisher Scientific (western blotting 1:1000, immunofluorescence 1:200); Monoclonal Anti-β-Tubulin I antibody produced in mouse, clone SAP.4G, T7816, Sigma-Aldrich (western blotting 1:20 000); E-Cadherin Antibody, AF748, R&D Systems (immunofluorescence 1:500); Calbindin D28K Antibody (C-20), sc-7691, Santa Cruz Biotechnology (immunofluorescence 1:400); Six2 Polyclonal antibody, 11562-1-AP, ProteinTech (immunofluorescence 1:500); AntiMonocarboxylate Transporter 1 Antibody, AB1286-I, Sigma-Aldrich (immunofluorescence 1:1000); Anti-Fibronectin Antibody, AB2033, Sigma-Aldrich (immunofluorescence 1:80); IRDye secondary antibodies, LI-COR Biosciences (western blotting 1:15 000); and Alexa Fluor secondary antibodies, Thermo Fisher Scientific (immunofluorescence 1:400). ..

    Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction.
    Article Snippet: .. For immunofluorescence colocalization studies, kidney sections were simultaneously probed with: (1) anti-C5aR2 (1:100; sc-515734; Santa Cruz Biotechnology), (2) the proximal tubule marker AQP-1 (1:200; ab168387; Abcam; a marker for PTECs), and (3) the distal tubule marker calbindin-D28K (1:100; 14479-1-AP; Proteintech; a marker for distal renal tubular epithelial cells). .. The samples were subsequently incubated with the species-matched fluorescent secondary antibodies Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; A32723; Invitrogen) and Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:200; A21207; Invitrogen).

    Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction
    Article Snippet: .. For immunofluorescence colocalization studies, kidney sections were simultaneously probed with: (1) anti-C5aR2 (1:100; sc-515734; Santa Cruz Biotechnology), (2) the proximal tubule marker AQP-1 (1:200; ab168387; Abcam; a marker for PTECs), and (3) the distal tubule marker calbindin-D28K (1:100; 14479-1-AP; Proteintech; a marker for distal renal tubular epithelial cells). .. The samples were subsequently incubated with the species-matched fluorescent secondary antibodies Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; A32723; Invitrogen) and Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:200; A21207; Invitrogen).

    Produced:

    Article Title: Embigin is involved in the regulation of early mouse kidney development
    Article Snippet: .. Following antibodies were used in our studies: Embigin Monoclonal Antibody, clone G7.43.1, 14-5839-81, eBioscience, Thermo Fisher Scientific (western blotting 1:1000, immunofluorescence 1:200); Monoclonal Anti-β-Tubulin I antibody produced in mouse, clone SAP.4G, T7816, Sigma-Aldrich (western blotting 1:20 000); E-Cadherin Antibody, AF748, R&D Systems (immunofluorescence 1:500); Calbindin D28K Antibody (C-20), sc-7691, Santa Cruz Biotechnology (immunofluorescence 1:400); Six2 Polyclonal antibody, 11562-1-AP, ProteinTech (immunofluorescence 1:500); Anti-Monocarboxylate Transporter 1 Antibody, AB1286-I, Sigma-Aldrich (immunofluorescence 1:1000); Anti-Fibronectin Antibody, AB2033, Sigma-Aldrich (immunofluorescence 1:80); IRDye secondary antibodies, LI-COR Biosciences (western blotting 1:15 000); and Alexa Fluor secondary antibodies, Thermo Fisher Scientific (immunofluorescence 1:400). ..

    Article Title: Embigin is involved in the regulation of early mouse kidney development.
    Article Snippet: .. AR TIC LE IN PR ES S Antibodies Following antibodies were used in our studies: Embigin Monoclonal Antibody, clone G7.43.1, 14-5839-81, eBioscience, Thermo Fisher Scientific (western blotting 1:1000, immunofluorescence 1:200); Monoclonal Anti-β-Tubulin I antibody produced in mouse, clone SAP.4G, T7816, Sigma-Aldrich (western blotting 1:20 000); E-Cadherin Antibody, AF748, R&D Systems (immunofluorescence 1:500); Calbindin D28K Antibody (C-20), sc-7691, Santa Cruz Biotechnology (immunofluorescence 1:400); Six2 Polyclonal antibody, 11562-1-AP, ProteinTech (immunofluorescence 1:500); AntiMonocarboxylate Transporter 1 Antibody, AB1286-I, Sigma-Aldrich (immunofluorescence 1:1000); Anti-Fibronectin Antibody, AB2033, Sigma-Aldrich (immunofluorescence 1:80); IRDye secondary antibodies, LI-COR Biosciences (western blotting 1:15 000); and Alexa Fluor secondary antibodies, Thermo Fisher Scientific (immunofluorescence 1:400). ..

    Incubation:

    Article Title: A decrease in Atp8a2 expression in Purkinje cells mediated acrylamide-induced cerebellar pathology in rats.
    Article Snippet: Atp8a2 is a type of phospholipid flippases, highly expressed in the cerebellum, functioning to maintain the stability and normal function of the cytomembrane by transporting phosphatidylserine into the cytoplasmic membrane.. Atp8a2 mutations and knockout can cause neuronal PS externalization and cerebellar ataxia.. The cerebellar damage caused by acrylamide (ACR) exposure has similar pathological features to the symptoms caused by Atp8a2 mutations and knockout.

    Article Title: Taurine attenuates arsenic-induced neurotoxicity through modulation of gut microbiota structure.
    Article Snippet: 1 College of Pharmaceutical Sciences, College of Chinese Medicine, Southwest University, Chongqing 400715, PR China 2 The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014010, PR China 3 Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014010, PR China Abstract Rationale Memory decline (MD) and anxiety/depression-like behavior (ADB) are multisystem diseases involving gastrointestinal and microbiota dysbiosis.. Long-term exposure to arsenic (As) has been shown to induce MD and ADB; however, the underlying mechanisms remain unknown.. Research findings suggest that taurine exhibits protective effects by regulating gut microbiota composition.

    Article Title: Bidirectional regulation of social memory by two distinct types of GABAergic neurons in the lateral septum.
    Article Snippet: Brains were dissected and post-fixed in 4% PFA for 6 h at 4 °C, then immersed in 30% sucrose for 2 days before sectioning (30 μm) on a cryostat (Leica CM1050; Leica Biosystems, Germany). .. For IHC, slices were pre-incubated in blocking solution (10% normal goat serum with 0.3% Triton X-100 in PBS) for 1 h at room temperature (RT) and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CB (1:500, #14479-1-AP; Proteintech, China), mouse anti-NeuN (1:1 000, #MAB377; Merck, Germany), guinea pig anti-c-fos (1:1 000, #226004; SYSY, Germany), rabbit anti-RGS14 (1:1 000, #16258-1-AP; Proteintech, China), rat antimCherry (1:1 000, #M11217; Thermo Fisher Scientific, USA), and rabbit anti-GFP (1:1 000, #A-11122; Thermo Fisher Scientific). .. The slices were washed in PBS (10 min, three times) and incubated with secondary antibodies (1:1 000, goat anti-rabbit Alexa Fluor 488, goat anti-rat Alexa Fluor 546, goat antiguinea pig Alexa Fluor 633, and goat anti-mouse Alexa Fluor 647; Invitrogen, USA) for 2 h at RT.

    Immunohistochemistry:

    Article Title: Bidirectional regulation of social memory by two distinct types of GABAergic neurons in the lateral septum.
    Article Snippet: Brains were dissected and post-fixed in 4% PFA for 6 h at 4 °C, then immersed in 30% sucrose for 2 days before sectioning (30 μm) on a cryostat (Leica CM1050; Leica Biosystems, Germany). .. For IHC, slices were pre-incubated in blocking solution (10% normal goat serum with 0.3% Triton X-100 in PBS) for 1 h at room temperature (RT) and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CB (1:500, #14479-1-AP; Proteintech, China), mouse anti-NeuN (1:1 000, #MAB377; Merck, Germany), guinea pig anti-c-fos (1:1 000, #226004; SYSY, Germany), rabbit anti-RGS14 (1:1 000, #16258-1-AP; Proteintech, China), rat antimCherry (1:1 000, #M11217; Thermo Fisher Scientific, USA), and rabbit anti-GFP (1:1 000, #A-11122; Thermo Fisher Scientific). .. The slices were washed in PBS (10 min, three times) and incubated with secondary antibodies (1:1 000, goat anti-rabbit Alexa Fluor 488, goat anti-rat Alexa Fluor 546, goat antiguinea pig Alexa Fluor 633, and goat anti-mouse Alexa Fluor 647; Invitrogen, USA) for 2 h at RT.

    Blocking Assay:

    Article Title: Bidirectional regulation of social memory by two distinct types of GABAergic neurons in the lateral septum.
    Article Snippet: Brains were dissected and post-fixed in 4% PFA for 6 h at 4 °C, then immersed in 30% sucrose for 2 days before sectioning (30 μm) on a cryostat (Leica CM1050; Leica Biosystems, Germany). .. For IHC, slices were pre-incubated in blocking solution (10% normal goat serum with 0.3% Triton X-100 in PBS) for 1 h at room temperature (RT) and incubated overnight at 4 °C with primary antibodies, including rabbit anti-CB (1:500, #14479-1-AP; Proteintech, China), mouse anti-NeuN (1:1 000, #MAB377; Merck, Germany), guinea pig anti-c-fos (1:1 000, #226004; SYSY, Germany), rabbit anti-RGS14 (1:1 000, #16258-1-AP; Proteintech, China), rat antimCherry (1:1 000, #M11217; Thermo Fisher Scientific, USA), and rabbit anti-GFP (1:1 000, #A-11122; Thermo Fisher Scientific). .. The slices were washed in PBS (10 min, three times) and incubated with secondary antibodies (1:1 000, goat anti-rabbit Alexa Fluor 488, goat anti-rat Alexa Fluor 546, goat antiguinea pig Alexa Fluor 633, and goat anti-mouse Alexa Fluor 647; Invitrogen, USA) for 2 h at RT.

    Marker:

    Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction.
    Article Snippet: .. For immunofluorescence colocalization studies, kidney sections were simultaneously probed with: (1) anti-C5aR2 (1:100; sc-515734; Santa Cruz Biotechnology), (2) the proximal tubule marker AQP-1 (1:200; ab168387; Abcam; a marker for PTECs), and (3) the distal tubule marker calbindin-D28K (1:100; 14479-1-AP; Proteintech; a marker for distal renal tubular epithelial cells). .. The samples were subsequently incubated with the species-matched fluorescent secondary antibodies Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; A32723; Invitrogen) and Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:200; A21207; Invitrogen).

    Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction
    Article Snippet: .. For immunofluorescence colocalization studies, kidney sections were simultaneously probed with: (1) anti-C5aR2 (1:100; sc-515734; Santa Cruz Biotechnology), (2) the proximal tubule marker AQP-1 (1:200; ab168387; Abcam; a marker for PTECs), and (3) the distal tubule marker calbindin-D28K (1:100; 14479-1-AP; Proteintech; a marker for distal renal tubular epithelial cells). .. The samples were subsequently incubated with the species-matched fluorescent secondary antibodies Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; A32723; Invitrogen) and Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:200; A21207; Invitrogen).



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    Image Search Results


    (A) Calbindin-labeled inferior olive somata with little intervening space (arrowheads). (B) PDX1-Cre × Ai9 dataset and soma-center map; orange points indicate somata with close neighbors. (C) Top, soma-equivalent diameters overlap the distribution of nearest-neighbor center distances, indicating that many somata lie close enough to contact. Bottom, close-neighbor prevalence was compared with packing-aware shuffled null datasets for each slice (S1–S8). Colored points show the prevalence measured from the real soma positions, and matching colored curves show the cumulative distributions obtained after shuffling soma positions within each slice footprint. The rightward displacement of real values relative to the shuffled distributions indicates that close somatic neighbors occur more often than expected from packing constraints alone. Scale bars: A, 50 µ m; B, 200 µ m.

    Journal: bioRxiv

    Article Title: Somatic Cx36 gap junctions expand the anatomical substrate for electrical coupling in the adult mouse inferior olive

    doi: 10.64898/2026.09.09.750283

    Figure Lengend Snippet: (A) Calbindin-labeled inferior olive somata with little intervening space (arrowheads). (B) PDX1-Cre × Ai9 dataset and soma-center map; orange points indicate somata with close neighbors. (C) Top, soma-equivalent diameters overlap the distribution of nearest-neighbor center distances, indicating that many somata lie close enough to contact. Bottom, close-neighbor prevalence was compared with packing-aware shuffled null datasets for each slice (S1–S8). Colored points show the prevalence measured from the real soma positions, and matching colored curves show the cumulative distributions obtained after shuffling soma positions within each slice footprint. The rightward displacement of real values relative to the shuffled distributions indicates that close somatic neighbors occur more often than expected from packing constraints alone. Scale bars: A, 50 µ m; B, 200 µ m.

    Article Snippet: Sections were incubated at 4°C for 48 h in antibody buffer (2% BSA and 0.4% Triton X-100 in PBS) containing mouse anti-Cx36 primary antibody (1:200 dilution, 2.5 μ g/mL, #37-4600, Thermo Fisher Invitrogen) and guinea pig anti-calbindin antibody (1 μ g/mL, #214 005, Synaptic Systems), with shaking at 80 rpm.

    Techniques: Labeling

    Confocal images of Calbindin-labeled olivary somata and Cx36 immunolabeling from multiple examples of closely apposed somata. Images are maximum-intensity projections of optical sections acquired at 0.1 µ m z-steps, with each projected substack spanning less than 2 µ m in z . For each example, single-channel Calbindin and Cx36 panels show the cellular context and punctate Cx36 signal, and the merged panels show Cx36 puncta concentrated at or immediately adjacent to the soma-soma interface. The repeated occurrence of puncta at close somatic contacts supports the presence of Cx36-containing junctional specializations beyond dendritic spines. Scale bars: 5 µ m.

    Journal: bioRxiv

    Article Title: Somatic Cx36 gap junctions expand the anatomical substrate for electrical coupling in the adult mouse inferior olive

    doi: 10.64898/2026.09.09.750283

    Figure Lengend Snippet: Confocal images of Calbindin-labeled olivary somata and Cx36 immunolabeling from multiple examples of closely apposed somata. Images are maximum-intensity projections of optical sections acquired at 0.1 µ m z-steps, with each projected substack spanning less than 2 µ m in z . For each example, single-channel Calbindin and Cx36 panels show the cellular context and punctate Cx36 signal, and the merged panels show Cx36 puncta concentrated at or immediately adjacent to the soma-soma interface. The repeated occurrence of puncta at close somatic contacts supports the presence of Cx36-containing junctional specializations beyond dendritic spines. Scale bars: 5 µ m.

    Article Snippet: Sections were incubated at 4°C for 48 h in antibody buffer (2% BSA and 0.4% Triton X-100 in PBS) containing mouse anti-Cx36 primary antibody (1:200 dilution, 2.5 μ g/mL, #37-4600, Thermo Fisher Invitrogen) and guinea pig anti-calbindin antibody (1 μ g/mL, #214 005, Synaptic Systems), with shaking at 80 rpm.

    Techniques: Confocal, Labeling, Immunolabeling