molecular identifier umi feature barcoding method (10X Genomics)
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10X Genomics
molecular identifier umi feature barcoding method
Molecular Identifier Umi Feature Barcoding Method, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding+methods/barcoding+feature/pm40579590-42-16-1
Average 86 stars, based on 1 article reviews
Molecular Identifier Umi Feature Barcoding Method, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding+methods/barcoding+feature/pm40579590-42-16-1
Average 86 stars, based on 1 article reviews
molecular identifier umi feature barcoding method - by Bioz Stars,
2026-09
86/100 stars
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Sequencing:Article Title: Comparative Analysis of Single-Nucleus and Single-Cell RNA Sequencing in Human Bone Marrow Mononuclear Cells: Methodological Insights and Trade-offs Article Snippet: .. This feature enables the application of methods like Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq), which utilises 10x Genomics’ Article Title: Integrated time-series analysis and high-content CRISPR screening delineate the dynamics of macrophage immune regulation. Article Snippet: .. CROP-seq screening: Single-cell sequencing Excess supernatant was reduced using the VR NxT instrument (Menarini Silicon Biosystems) and processed using the Chromium Next GEM Single Cell 5’ v2 Dual Indexing Kit with Single Cell:Article Title: BACH2 shapes tissue resident memory T cell programs to promote HIV-1 persistence Article Snippet: Raw fastq sequences from Chromium Single Cell Multiome ATAC + Gene Expression sequencing (ATAC & RNA) were demultiplexed with CellRanger-Arc v2 (10x Genomics) mkfastq and sequences were aligned to the hg38 reference genome using CellRanger-Arc count. .. Raw fastq sequences from Single Cell Gene Expression with Article Title: Tissue-adapted NK cells shape pathogenic cDC1 niches in early arthritis Article Snippet: .. Innate lymphoid cell libraries were generated using the Chromium Next GEM Single Cell 5’ v2 kit with Article Title: Neutrophils drive vascular occlusion, tumour necrosis and metastasis Article Snippet: Tumours were digested as previously stated, sorted for viability and stained with TotalSeq-B Mouse Universal Cocktail, V1.0 (199902, BioLegend), TotalSeq-B0109 anti-mouse CD16/32 antibody (101345, BioLegend) and TotalSeq-B0301-5 anti-mouse Hashtag 3 (BioLegend), all according to the manufacturer’s instructions. .. Samples were then used as input into the Chromium 3′ single-cell kit for Article Title: Human LFA-1 governs T cell immune surveillance of the skin Article Snippet: Cells were washed three times with PBS supplemented with 0.2% BSA and subjected to single-cell capture on the 10X Genomics Chromium platform. .. Libraries were generated with the Chromium Next GEM Automated Single-Cell 5′ Kit v2 with Article Title: Integrated time-series analysis and high-content CRISPR screening delineate the dynamics of macrophage immune regulation. Article Snippet: .. CROP-seq screening: Single-cell sequencing Excess supernatant was reduced using the VR NxT instrument (Menarini Silicon Biosystems) and processed using the Chromium Next GEM Single Cell 5’ v2 Dual Indexing Kit with Article Title: Spontaneous network activity links misrouted interneuron migration to cortical maturation abnormalities Article Snippet: A papain dissociation system (Worthington, LK003150) was used as described ( ) on the gentleMACS Octo Dissociator (Miltenyi Biotec) to generate a cell suspension. tdT + cells were sorted on BD FACSAria III (BD FACSDiva Software, v.8.0.2) with a 100 μm nozzle and subjected to downstream processing on the 10x Genomics Chromium platform. .. After sorting in PBS (Lonza, 17-516) with 0.02% BSA (B9000, NEB), ∼12’000 individual cells per sample were loaded onto a 10X Genomics Chromium platform for gel beads-in-emulsion and complementary DNA generation, carrying cell- and transcript-specific barcodes using the Chromium Single Cell 3’ Reagent Kit v.3.1 with Gene Expression:Article Title: BACH2 shapes tissue resident memory T cell programs to promote HIV-1 persistence Article Snippet: Raw fastq sequences from Chromium Single Cell Multiome ATAC + Gene Expression sequencing (ATAC & RNA) were demultiplexed with CellRanger-Arc v2 (10x Genomics) mkfastq and sequences were aligned to the hg38 reference genome using CellRanger-Arc count. .. Raw fastq sequences from Single Cell Gene Expression with Generated:Article Title: Tissue-adapted NK cells shape pathogenic cDC1 niches in early arthritis Article Snippet: .. Innate lymphoid cell libraries were generated using the Chromium Next GEM Single Cell 5’ v2 kit with Article Title: Human LFA-1 governs T cell immune surveillance of the skin Article Snippet: Cells were washed three times with PBS supplemented with 0.2% BSA and subjected to single-cell capture on the 10X Genomics Chromium platform. .. Libraries were generated with the Chromium Next GEM Automated Single-Cell 5′ Kit v2 with |
