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double-barcode pcr method  (Illumina Inc)


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    Structured Review

    Illumina Inc double-barcode pcr method
    Double Barcode Pcr Method, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/barcoding+methods/barcode/pm39482578-103-12-16
    Average 90 stars, based on 1 article reviews
    double-barcode pcr method - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Life stage and vaccination shape the gut microbiome of hatchery-reared Atlantic salmon (Salmo salar) intended for river stocking
    Article Snippet: .. Each sample was amplified with two pairs of barcodes to generate two technical replicates in Illumina sequencing. ..

    Sequencing:

    Article Title: Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth - A preliminary study.
    Article Snippet: As ruminants are frequently affected by periodontal diseases, understanding their microbial communities is crucial.. In this pilot study, we analyzed subgingival biofilm samples of young cattle across different states: clinically healthy (n = 5), gingivitis (n = 5), and periodontitis (n = 5) using 16S rRNA gene sequencing and cooccurrence network analysis.. The findings revealed that Proteobacteria was the predominant phylum across all conditions, with Fusobacteriota constituting 27.6 % of the microbiota in periodontitis-affected sites.

    Article Title: African ancestry neurodegeneration risk variant disrupts an intronic branchpoint in GBA1
    Article Snippet: .. As an additional quality control, we checked barcode sequence diversity in each of our cell types by calculating the ratio of barcodes in our data divided by total Illumina barcodes. ..

    Polymerase Chain Reaction:

    Article Title: Planting density effect on poplar growth traits and soil nutrient availability, and response of microbial community, assembly and function.
    Article Snippet: .. The V5-V7 region of bacterial 16 S rDNA was sequenced using a double-barcode PCR method via Illumina sequencing, enabling the identification and taxonomic annotation of cultivable bacteria. ..

    Article Title: Genome-wide quantification of polycistronic transcription in Leishmania major .
    Article Snippet: Leishmania major is a human-pathogenic, obligate parasite and the etiological agent of the most prevalent, cutaneous form of leishmaniasis, which is an important neglected, tropical disease with ~1.2 million new infections per year.. Leishmania, and the whole order Trypanosomatida, are early eukaryotes with highly diverged gene expression and regulation pathways, setting them apart from their mammalian hosts and from most other eukaryotes.. Using precision run-on sequence analysis, we performed a genome-wide mapping and density analysis of RNA polymera ses in isolated nuclei of the protozoan parasite Leishmania major.

    Bacteria:

    Article Title: Planting density effect on poplar growth traits and soil nutrient availability, and response of microbial community, assembly and function.
    Article Snippet: .. The V5-V7 region of bacterial 16 S rDNA was sequenced using a double-barcode PCR method via Illumina sequencing, enabling the identification and taxonomic annotation of cultivable bacteria. ..

    other:

    Article Title: In vitro HIV DNA integration in STAT3 drives T cell persistence-A model of HIV-associated T cell lymphoma.
    Article Snippet: Following a method like that previously described [44,55], a linker was ligated to the DNA fragments, which were amplified and barcoded for Illumina sequencing.

    Control:

    Article Title: African ancestry neurodegeneration risk variant disrupts an intronic branchpoint in GBA1
    Article Snippet: .. As an additional quality control, we checked barcode sequence diversity in each of our cell types by calculating the ratio of barcodes in our data divided by total Illumina barcodes. ..



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    Image Search Results


    Journal: bioRxiv

    Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes

    doi: 10.1101/2025.06.16.659891

    Figure Lengend Snippet:

    Article Snippet: The 16S Barcoding Kit library preparation method was also able to provide consistent microbiome profiles in which Wolbachia was the dominant species which is consistent with previous profiles using 16S rRNA Illumina amplicon sequencing .

    Techniques: Sequencing

    All bar graphs depict sequencing runs using two different barcoding kits: the Rapid PCR Barcoding Kit 24 V14 (runs 2-6) and the 16S Barcoding Kit 24 V14 (runs 7,8,9,11,12). Each run is represented by a vertical column. A ) Total Data generated (GB). B ) Percentage of passed vs failed bases called for each run. C ) Total number of reads obtained in each sequencing run. D ) Percentage of unclassified passed reads and unclassified passed bases for each run.

    Journal: bioRxiv

    Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes

    doi: 10.1101/2025.06.16.659891

    Figure Lengend Snippet: All bar graphs depict sequencing runs using two different barcoding kits: the Rapid PCR Barcoding Kit 24 V14 (runs 2-6) and the 16S Barcoding Kit 24 V14 (runs 7,8,9,11,12). Each run is represented by a vertical column. A ) Total Data generated (GB). B ) Percentage of passed vs failed bases called for each run. C ) Total number of reads obtained in each sequencing run. D ) Percentage of unclassified passed reads and unclassified passed bases for each run.

    Article Snippet: The 16S Barcoding Kit library preparation method was also able to provide consistent microbiome profiles in which Wolbachia was the dominant species which is consistent with previous profiles using 16S rRNA Illumina amplicon sequencing .

    Techniques: Sequencing, Generated

    Libraries prepared and sequenced using either the Rapid PCR Barcoding Kit 24 V14 (Rapid) or 16S Barcoding Kit 24 V14 (16S).

    Journal: bioRxiv

    Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes

    doi: 10.1101/2025.06.16.659891

    Figure Lengend Snippet: Libraries prepared and sequenced using either the Rapid PCR Barcoding Kit 24 V14 (Rapid) or 16S Barcoding Kit 24 V14 (16S).

    Article Snippet: The 16S Barcoding Kit library preparation method was also able to provide consistent microbiome profiles in which Wolbachia was the dominant species which is consistent with previous profiles using 16S rRNA Illumina amplicon sequencing .

    Techniques: